{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T21:52:31Z","timestamp":1772315551931,"version":"3.50.1"},"reference-count":39,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,11,2]],"date-time":"2023-11-02T00:00:00Z","timestamp":1698883200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The aim of this study was to investigate the relationship between the levels of insulin\u2010like growth factor\u20101 (IGF\u20101) in serum and seminal plasma and the characteristics of semen in Beetal bucks (<jats:italic>Capra hircus<\/jats:italic>). A total of 12 adult Beetal bucks were involved in the study, with each buck providing six ejaculates collected using a standard artificial vagina (<jats:italic>n<\/jats:italic>\u2009=\u200972 total). Only qualified semen samples (volume of 0.7\u2009mL, a mass motility rating of 3+ or higher on a 0\u2013+ scale, and individual progressive motility of 80% or more) divided into three fractions were processed for estimation of IGF\u20101 and other seminal parameters like motility, viability, acrosome integrity, sperm abnormality and superoxide dismutase (SOD) activity. The first and second fraction were diluted and extended with Optixcell extender (1:15 ratio). The first ejaculate fraction was processed for studying fresh semen parameters and the second fraction was cryopreserved for evaluating frozen semen parameters. French mini straws (0.25\u2009mL) were used for semen filling, and polyvinyl alcohol powder of different colours was used for sealing the extended semen. The third fraction of each ejaculate was centrifuged at room temperature (1100\u2009\u00d7\u2009g for 7\u2009min) to separate the seminal plasma. Additionally, blood samples were taken from each buck on the same day as semen collection, resulting in a total of 36 blood samples. The results revealed a significant positive correlation (<jats:italic>r<\/jats:italic>\u2009=\u2009.4243; <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05) between the concentration of IGF\u20101 in both serum and seminal plasma of the Beetal bucks. Furthermore, the concentration of IGF\u20101 in serum showed significant positive correlations with sperm viability (<jats:italic>r<\/jats:italic>\u2009=\u2009.554; <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05), acrosome integrity (<jats:italic>r<\/jats:italic>\u2009=\u2009.527; <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05), post\u2010thaw sperm motility (<jats:italic>r<\/jats:italic>\u2009=\u2009.407; <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.01), post\u2010thaw sperm viability (<jats:italic>r<\/jats:italic>\u2009=\u2009.426; <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.01) and post\u2010thaw acrosome integrity (<jats:italic>r<\/jats:italic>\u2009=\u2009.333; <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). However, it had a significant negative correlation with SOD activity in fresh semen (<jats:italic>r<\/jats:italic>\u2009=\u2009\u22120.458; <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.01). Moreover, the concentration of IGF\u20101 in seminal plasma demonstrated significant positive correlations with individual progressive motility (<jats:italic>r<\/jats:italic>\u2009=\u2009.341; <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05), sperm viability (<jats:italic>r<\/jats:italic>\u2009=\u2009.527; <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05), acrosome integrity (<jats:italic>r<\/jats:italic>\u2009=\u2009.539; <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05), sperm plasma membrane integrity (<jats:italic>r<\/jats:italic>\u2009=\u2009.464; <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05), post\u2010thaw sperm motility (<jats:italic>r<\/jats:italic>\u2009=\u2009.644; <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.01), post\u2010thaw sperm viability (<jats:italic>r<\/jats:italic>\u2009=\u2009.643; <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.01), post\u2010thaw acrosome integrity (<jats:italic>r<\/jats:italic>\u2009=\u2009.487; <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.01) and post\u2010thaw sperm plasma membrane integrity (<jats:italic>r<\/jats:italic>\u2009=\u2009.521; <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.01). Additionally, it showed a significant negative correlation with SOD activity in both fresh semen (<jats:italic>r<\/jats:italic>\u2009=\u2009\u22120.714; <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.01) and frozen semen (<jats:italic>r<\/jats:italic>\u2009=\u2009\u22120.558; <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.01) of Beetal bucks. Based on these findings, IGF\u20101 in seminal plasma can be considered as a potential biomarker for the selection of bucks for breeding purposes.<\/jats:p>","DOI":"10.1111\/rda.14499","type":"journal-article","created":{"date-parts":[[2023,11,2]],"date-time":"2023-11-02T16:54:36Z","timestamp":1698944076000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Association between levels of insulin\u2010like growth factor\u20101 in serum and seminal plasma with fresh and frozen\u2010thawed semen characteristics in Beetal bucks"],"prefix":"10.1111","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-4042-8113","authenticated-orcid":false,"given":"Keshav","family":"Kumar","sequence":"first","affiliation":[{"name":"Department of Animal Reproduction, Gynaecology and Obstetrics College of Veterinary Science, Assam Agricultural University  Guwahati 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