{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T17:30:14Z","timestamp":1787506214073,"version":"build-2736575974"},"reference-count":41,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,12,7]],"date-time":"2023-12-07T00:00:00Z","timestamp":1701907200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Testicular development and spermatogenesis are complex phenomena controlled by various genetic factors, including miRNA\u2010based post\u2010transcriptional gene expression regulation. Exploring the miRNA expression patterns during testicular development in Dezhou donkeys would enhance our understanding of equine fertility and spermatogenesis. In this investigation, we examined the testicular miRNA profiles at various stages of development. The experimental animals were divided into three groups based on their developmental stages: 2\u2009months old (juvenile:\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20093), 12\u2009months old (adolescent;\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20093) and 24\u2009months old (adult;\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20093) donkeys. Total RNA was extracted from dissected testicles for miRNA sequencing and analysis. In total, 586 miRNAs, including 451 known miRNAs and 135 novel miRNAs, were identified. Among identified miRNAs, 315 displayed age\u2010dependent expression differences. The levels of miRNA expression in the juvenile group were significantly higher than in the adolescent or adult groups. The MiR\u2010483 exhibited the maximum fold change between juvenile and adolescent groups. Several screened genes, including\n                    <jats:italic>SLC45A4<\/jats:italic>\n                    and\n                    <jats:italic>TFCP2L1<\/jats:italic>\n                    , have been linked to male reproductive pathways in donkeys. In addition, miR\u2010744 was predicted to regulate\n                    <jats:italic>SPIN2B<\/jats:italic>\n                    , a gene implicated in spermatocyte cell cycle progression and genomic integrity of spermatozoa. These results contribute to our comprehension of microRNA regulation during testicular development and spermatogenesis in Dezhou donkeys. The identified microRNAs and their target genes have the potential to serve as biomarkers for evaluating the reproductive capacity of stud donkeys.\n                  <\/jats:p>","DOI":"10.1111\/rda.14502","type":"journal-article","created":{"date-parts":[[2023,12,7]],"date-time":"2023-12-07T21:20:01Z","timestamp":1701984001000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Exploring testicular miRNA profiles during developmental stages in Dezhou donkeys: A preliminary insight"],"prefix":"10.1111","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-2610-4539","authenticated-orcid":false,"given":"Zulfiqar","family":"Ahmed","sequence":"first","affiliation":[{"name":"Network and Information Center Northwest A&amp;F University  Yangling China"},{"name":"Faculty of Veterinary and Animal Sciences University of Poonch  Rawalakot Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Liu","sequence":"additional","affiliation":[{"name":"Network and Information Center Northwest A&amp;F University  Yangling China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Yu","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Gelatin\u2010based Traditional Chinese Medicine Dong\u2010E\u2010E\u2010Jiao Co. 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Dong\u2010E Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Dong","sequence":"additional","affiliation":[{"name":"Institute of Animal Science Xinjiang Academy of Animal Sciences  Urumqi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6335-9633","authenticated-orcid":false,"given":"Zahid","family":"Naseer","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary and Animal Sciences Pir Mehr Ali Shah Arid Agriculture University  Rawalpindi Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Imtiaz","family":"Ahmad","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary and Animal Sciences University of Poonch  Rawalakot Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Imran","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary and Animal Sciences University of Poonch  Rawalakot 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