{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T07:09:52Z","timestamp":1787728192720,"version":"build-2784847793"},"reference-count":26,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,12,6]],"date-time":"2023-12-06T00:00:00Z","timestamp":1701820800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The aim of this study was to evaluate alpaca pregnancy outcomes and birth rates of females inseminated with frozen semen using two commercial extenders. A total of 18 ejaculates from 8 adult alpaca males were obtained with artificial vagina, and macroscopic and microscopic semen characteristics were assessed. Afterwards, samples were divided into two aliquots, diluted with Biladyl\u00ae B or AndroMed\u00ae, and cooled for 2\u2009h at 5\u00b0C. At that moment, sperm motility was evaluated, and samples were frozen through a gradual descent of temperature using a liquid nitrogen tank. To analyse frozen sperm quality, samples were thawed at 38\u00b0C for 30\u2009s. Even though a significant decrease in sperm motility and viability was detected when thawed (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05), no superiority was found between the two commercial extenders (Biladyl\u00ae B vs. AndroMed\u00ae). A total of 36 alpaca females were artificially inseminated (AI) between 30 and 34\u2009h post\u2010injection of a GnRH analogue, administered when a growing dominant follicle was detected through transrectal palpation and ultrasonography. Obtained pregnancy rates were similar between Biladyl\u00ae B (33.3%, 6\/18) and AndroMed\u00ae (22.2%, 4\/18). No significant differences were detected in birth rates between the two tested extenders, obtaining 4 and 3 births for Biladyl\u00ae and AndroMed\u00ae, respectively. In conclusion, alpaca pregnancies and alive offspring can be obtained through AI with frozen semen at similar efficiency rates using commercial diluents, Biladyl\u00ae B or AndroMed\u00ae.<\/jats:p>","DOI":"10.1111\/rda.14514","type":"journal-article","created":{"date-parts":[[2023,12,6]],"date-time":"2023-12-06T17:54:58Z","timestamp":1701885298000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Pregnancy and birth rate outcomes in alpacas (<i>Vicugna pacos<\/i>) inseminated with frozen semen using two commercial extenders"],"prefix":"10.1111","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8717-9335","authenticated-orcid":false,"given":"M. Contreras","family":"Huamani","sequence":"first","affiliation":[{"name":"Laboratorio de Biotecnolog\u00eda Reproductiva, Estaci\u00f3n Experimental Agraria Cana\u00e1n Instituto Nacional de Innovaci\u00f3n Agraria  Ayacucho Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0488-757X","authenticated-orcid":false,"given":"C. A.","family":"Olaguivel\u00a0Flores","sequence":"additional","affiliation":[{"name":"Laboratorio de Fisiolog\u00eda Veterinaria, Medicina Veterinaria Universidad Nacional de San Crist\u00f3bal de Huamanga  Ayacucho Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6177-5844","authenticated-orcid":false,"given":"C. Y. Guillen","family":"Palomino","sequence":"additional","affiliation":[{"name":"Laboratorio de Biotecnolog\u00eda Reproductiva, Estaci\u00f3n Experimental Agraria Cana\u00e1n Instituto Nacional de Innovaci\u00f3n Agraria  Ayacucho Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4997-0580","authenticated-orcid":false,"given":"M. I.","family":"Carretero","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n y Tecnolog\u00eda en Reproducci\u00f3n Animal, Facultad de Ciencias Veterinarias Universidad de Buenos Aires, Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas  Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4561-9005","authenticated-orcid":false,"given":"F. G.","family":"Fumuso","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine University of Minnesota  Saint Paul Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I. M. Laines","family":"Arcce","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Fisiologia e Controle da Reprodu\u00e7\u00e3o, Faculdade de Veterin\u00e1ria Universidade Estadual do Cear\u00e1 (UECE)  Fortaleza Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,12,6]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2021.611301"},{"key":"e_1_2_10_3_1","first-page":"15","article-title":"Influencia de la criopreservaci\u00f3n sobre la motilidad, viabilidad y fertilidad de espermatozoides de llama (Lama glama)","volume":"52","author":"Aller J. F.","year":"2003","journal-title":"Archivos de Zootecnia"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2011.02.020"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2022.107073"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378\u20104320(00)00158\u20105"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.12319"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439\u20100272.2011.01186.x"},{"key":"e_1_2_10_9_1","unstructured":"Deza Calsin H. &Pacheco Curie J.(2019).Efecto del almacenamiento de espermatozoides congelados de llama por un largo per\u00edodo de tiempo sobre la motilidad vitalidad y capacidad fecundante al descongelado.13\u00b0 Simposio Internacional de Reproducci\u00f3n Animal(p. 365). Agosto 28\u201330. 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