{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T04:53:35Z","timestamp":1790744015184,"version":"4.1.0"},"reference-count":81,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,12,26]],"date-time":"2023-12-26T00:00:00Z","timestamp":1703548800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002383","name":"King Saud University","doi-asserted-by":"publisher","award":["RSPD2023R971"],"award-info":[{"award-number":["RSPD2023R971"]}],"id":[{"id":"10.13039\/501100002383","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    This study aimed to determine the effects of Coenzyme Q10 (CoQ10) in the freezing medium on functional and oxidative stress parameters and in\u00a0vitro fertilization (IVF) rate of buffalo sperm. Collected samples were relocated to the laboratory for initial evaluation, gentle dilution in extenders, cooling (4\u00b0C, 2\u2009h), equilibration (4\u00b0C, 4\u2009h), packaging (straws, 0.5\u2009mL), programmable freezing, and thawing (37\u00b0C, 30\u2009s). Statistical analysis depicted that adding CoQ10 (100\u2009\u03bcM) in a freezing medium caused a significant augmentation in total motility (%), average path, and straight\u2010line velocities (\u03bcm\/sec) of buffalo sperm than control. Adding CoQ10 (100\u2009\u03bcM) improved sperm progressive motility, rapid velocity, and functional parameters (%) compared to the control and 10\u2009\u03bcM of CoQ10. Moreover, CoQ10 in a freezing medium caused a significant augmentation in seminal plasma catalase (U\/mL) and glutathione reductase (GSH; nmol\/10\n                    <jats:sup>9<\/jats:sup>\n                    ) at 100\u2009\u03bcM than control and other treatments. CoQ10 inclusion (100\u2009\u03bcM) ameliorates seminal plasma superoxide dismutase (U\/mL), glutathione\u2010S\u2010transferase (GST; nmol\/mL\/min) fructose (\u03bcg\/mL), and ATP (nmol\/million) than control. Furthermore, CoQ10 at 100\u2009\u03bcM improved seminal plasma glutathione peroxidase (\u03bcM) levels than control, 10\u2009\u03bcM, and 20\u2009\u03bcM. Lastly, hydrogen peroxide (H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2;<\/jats:sub>\n                    nM) production was significantly lower at 100\u2009\u03bcM than at control and 10\u2009\u03bcM. CoQ10 (100\u2009\u03bcM) caused a significant augmentation in the un\u2010capacitated pattern followed by a reduction in the capacitated pattern, and apoptosis\u2010like changes (%) than control, and other treatments, whereas viability was increased than control and other treatments. CoQ10 (100\u2009\u03bcM) significantly improved the IVF rate in comparison with control, CoQ10 at 10\u2009\u03bcM, and 20\u2009\u03bcM groups. In conclusion, the addition of CoQ10 (100\u2009\u03bcM) in the freezing medium can improve the quality and in\u00a0vitro fertility of post\u2010thawed buffalo semen via its antioxidative effect. Further studies are needed to evaluate the effect of CoQ10 on the in\u00a0vivo fertility of buffalo bull semen.\n                  <\/jats:p>","DOI":"10.1111\/rda.14515","type":"journal-article","created":{"date-parts":[[2023,12,26]],"date-time":"2023-12-26T03:44:40Z","timestamp":1703562280000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Coenzyme Q10 improves the quality and in\u00a0vitro fertility of post\u2010thawed buffalo (\n                    <i>Bubalus bubalis<\/i>\n                    ) semen via its antioxidative effect"],"prefix":"10.1111","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3180-8208","authenticated-orcid":false,"given":"Hussain","family":"Ahmed","sequence":"first","affiliation":[{"name":"Department of Zoology University of Buner  Khyber Pakhtunkhwa KP Pakistan"},{"name":"Department of Animal Sciences, Reproductive Physiology Laboratory Quaid\u2010i\u2010Azam University, Campus  Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Umar","family":"Ijaz","sequence":"additional","affiliation":[{"name":"Department of Zoology, Wildlife and Fisheries University of Agriculture  Faisalabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarwat","family":"Jahan","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, Reproductive Physiology Laboratory Quaid\u2010i\u2010Azam University, Campus  Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehreen","family":"Riaz","sequence":"additional","affiliation":[{"name":"Department of Zoology Women University Swabi  Swabi KP Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haney","family":"Samir","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine Tokyo University of Agriculture and Technology  Fuchu Japan"},{"name":"Department of Theriogenology, Faculty of Veterinary Medicine Cairo University  Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3247-5898","authenticated-orcid":false,"given":"Ayman A.","family":"Swelum","sequence":"additional","affiliation":[{"name":"Department of Animal Production, College of Food and Agriculture Science King Saud University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,12,26]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1126903"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11626-016-0061-x"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.106.9.199"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2016.05.010"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2022.05.004"},{"issue":"2","key":"e_1_2_10_7_1","first-page":"106","article-title":"Effect of different concentrations of fructose and glycerol in tris citric acid extender on post thaw quality and fertility of buffalo bull spermatozoa","volume":"41","author":"Ahmed H.","year":"2020","journal-title":"CryoLetters"},{"issue":"3","key":"e_1_2_10_8_1","first-page":"173","article-title":"Effect of cryopreservation on CASA characteristics, mitochondrial transmembrane potential, plasma and acrosome integrities, morphology and in\u00a0vivo fertility of buffalo bull spermatozoa","volume":"40","author":"Ahmed H.","year":"2019","journal-title":"CryoLetters"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.2164\/jandrol.112.016535"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.5534\/wjmh.190145"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.1992.tb00306.x"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2008.01240.x"},{"issue":"6","key":"e_1_2_10_13_1","first-page":"493","article-title":"Role of sperm apoptosis and oxidative stress in male infertility: A narrative review","volume":"19","author":"Asadi A.","year":"2021","journal-title":"International Journal of Reproductive BioMedicine"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2008.06.014"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF03346521"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.4061\/2011\/686137"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.2000.tb03420.x"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2012.06.007"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2009.07.988"},{"key":"e_1_2_10_20_1","first-page":"112","volume-title":"Glutathione in red blood cell metabolism. 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