{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T00:57:22Z","timestamp":1772931442377,"version":"3.50.1"},"reference-count":20,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,12,27]],"date-time":"2023-12-27T00:00:00Z","timestamp":1703635200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Sterilization of the culture medium using ultraviolet (UV)\u2010C reduces the potential adverse effects of microorganisms and allows for long\u2010term use. In the present study, we investigated the effects of a medium directly irradiated with UV\u2010C prior to in\u00a0vitro culture on the development and quality of porcine in\u00a0vitro\u2010fertilized embryos and the free amino acid composition of the culture media. The culture media (porcine zygote medium [PZM\u20105] and porcine blastocyst medium [PBM]) were irradiated with UV\u2010C at 228 and 260\u2009nm for 1 and 3\u2009days, respectively. Next, the culture media were irradiated with UV\u2010C at 228\u2009nm for 3, 7, or 14\u2009days. After in\u00a0vitro fertilization, the embryos were cultured in the UV\u2010C\u2010irradiated media for 7\u2009days. Free amino acid levels in culture media irradiated with 228 and 260\u2009nm UV\u2010C for 3\u2009days were analysed. The blastocyst formation rate of embryos cultured in media irradiated with 260\u2009nm UV\u2010C for 3\u2009days was significantly lower than that of embryos cultured in non\u2010irradiated control media. However, 228\u2009nm UV\u2010C irradiation for up to 14\u2009days did not affect blastocyst formation rates and quality in the resulting blastocysts. Moreover, 260\u2009nm UV\u2010C irradiation significantly increased the taurine concentration in both culture media and decreased methionine concentration in the PBM. In conclusion, UV\u2010C irradiation at 228\u2009nm before in\u00a0vitro culture had no detrimental effects on embryonic development. However, 260\u2009nm UV\u2010C irradiation decreased embryo development and altered the composition of free amino acids in the medium.<\/jats:p>","DOI":"10.1111\/rda.14520","type":"journal-article","created":{"date-parts":[[2023,12,27]],"date-time":"2023-12-27T11:38:48Z","timestamp":1703677128000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Development of porcine embryos cultured in media irradiated with <scp>ultraviolet\u2010C<\/scp>"],"prefix":"10.1111","volume":"59","author":[{"given":"Nanaka","family":"Torigoe","sequence":"first","affiliation":[{"name":"Bio\u2010Innovation Research Center Tokushima University  Tokushima Japan"},{"name":"Faculty of Bioscience and Bioindustry Tokushima University  Tokushima 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