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The aim of this study was to evaluate the effect of in\u00a0vitro fertilization medium supplementation with 5% or 10% bovine follicular fluid (BFF) on the production of in\u00a0vitro bovine embryos. FF was collected from ovarian follicles with a diameter of 8\u201310\u2009mm, and cumulus\u2013oocyte complexes (COCs) were co\u2010incubated with sperm for 24\u2009h in the commercial medium BotuFIV\u00ae (BotuPharma\u00a9), being distributed among the experimental groups: oocytes fertilized in a control medium; oocytes fertilized in a medium supplemented with 5% BFF; and oocytes fertilized in a medium supplemented with 10% BFF. After fertilization, the zygotes were cultured in\u00a0vitro for 8\u2009days. Embryo development was assessed through cleavage rates (day 2) and blastocyst formation rates (day 8). The relative expression of the genes OCT4, IFNT2, BAX, HSP70 and SOD2 was measured using the real\u2010time polymerase chain reaction method. There was no difference (<jats:italic>p<\/jats:italic>\u2009&gt;\u2009.05) among the different experimental groups in terms of cleavage rates and blastocyst formation rates. Regarding the gene expression results, only the blastocysts from oocytes fertilized with 10% BFF showed significantly lower expression of IFNT2 (<jats:italic>p<\/jats:italic>\u2009=\u2009.003) and SOD2 (<jats:italic>p<\/jats:italic>\u2009=\u2009.01) genes compared to blastocysts from oocytes fertilized in control medium alone, while there was no difference between blastocyst from oocytes fertilized in control medium and the ones from oocytes fertilized with 5% BFF. In addition to this, the blastocysts from oocytes fertilized with 5% BFF showed significantly reduced levels of expression of the heat shock protein HSP70 (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.001) and the pro\u2010apoptotic protein BAX (<jats:italic>p<\/jats:italic>\u2009=\u2009.015) compared to blastocysts from oocytes fertilized with control medium. This may indicate that lower supplementation of BFF to the IVF medium creates a more suitable environment for fertilization and is less stressful for the zygote.<\/jats:p>","DOI":"10.1111\/rda.14548","type":"journal-article","created":{"date-parts":[[2024,3,9]],"date-time":"2024-03-09T10:45:53Z","timestamp":1709981153000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Fertilization with follicular fluid reduces HSP70 and BAX expression on bovine in\u00a0vitro embryos"],"prefix":"10.1111","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5179-8812","authenticated-orcid":false,"given":"Bruno Porpino","family":"Homobono","sequence":"first","affiliation":[{"name":"UFPA \u2010 Universidade Federal do Par\u00e1 \u2010 R. Augusto Corr\u00eaa  Bel\u00e9m \u2013 PA Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcela Oliveira","family":"das Merc\u00eas","sequence":"additional","affiliation":[{"name":"UFRA \u2010 Universidade Federal Rural da Amaz\u00f4nia \u2013 Estr  Bel\u00e9m \u2013 PA Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7655-821X","authenticated-orcid":false,"given":"Leonardo Henrique da Silva","family":"Nogueira","sequence":"additional","affiliation":[{"name":"UFPA \u2010 Universidade Federal do Par\u00e1 \u2010 R. Augusto Corr\u00eaa  Bel\u00e9m \u2013 PA Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8777-2059","authenticated-orcid":false,"given":"Eduardo Baia","family":"de Souza","sequence":"additional","affiliation":[{"name":"UFPA \u2010 Universidade Federal do Par\u00e1 \u2010 R. 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