{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T16:02:08Z","timestamp":1785600128284,"version":"3.56.0"},"reference-count":44,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,4,2]],"date-time":"2024-04-02T00:00:00Z","timestamp":1712016000000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We aimed to evaluate the effects of rumen\u2010protected lysine (RPL) supplementation during the close\u2010up period on uterine involution and the resumption of ovarian function in dairy cows. Fifty\u2010two multiparous Holstein cows were categorized based on parity and expected calving date and randomly assigned to the RPL or control (CON) groups. The RPL group received 80\u2009g of RPL daily from day 21 before the expected calving date until parturition. Blood samples were obtained twice weekly from pre\u2010supplementation to 6\u2009weeks postpartum. The onset of luteal activity postpartum was determined via ultrasonography twice weekly for up to 6\u2009weeks postpartum. Uterine involution was tracked at 3 and 5\u2009weeks postpartum through the vaginal discharge score, percentage of polymorphonuclear cells (PMN) in endometrial cytology samples, presence of intrauterine fluid, and gravid horn diameter via ultrasonography. Before supplementation, the RPL group showed amino acid imbalance, which was improved by RPL supplementation. There were no significant differences in the onset of luteal activity, percentage of PMN, intrauterine fluid, or the diameter of the uterine horn between the two groups. The vaginal discharge score in the RPL group decreased from 3 to 5\u2009weeks postpartum, whereas that in the CON groups did not decrease. The number of cows with clinical endometritis was lower in the RPL group. Overall, RPL supplementation during the close\u2010up period enhanced vaginal discharge clearance, potentially averting clinical endometritis, but did not affect the first ovulation in dairy cows.<\/jats:p>","DOI":"10.1111\/rda.14558","type":"journal-article","created":{"date-parts":[[2024,4,3]],"date-time":"2024-04-03T05:05:27Z","timestamp":1712120727000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Supplementation of rumen\u2010protected lysine during the close\u2010up period improves vaginal discharge clearance in Holstein dairy cows"],"prefix":"10.1111","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4629-2013","authenticated-orcid":false,"given":"Chiho","family":"Kawashima","sequence":"first","affiliation":[{"name":"Field Center of Animal Science and Agriculture Obihiro University of Agriculture and Veterinary Medicine  Obihiro Hokkaido Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hina","family":"Hayakawa","sequence":"additional","affiliation":[{"name":"Field Center of Animal Science and Agriculture Obihiro University of Agriculture and Veterinary Medicine  Obihiro Hokkaido Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aki","family":"Taniguchi","sequence":"additional","affiliation":[{"name":"Field Center of Animal Science and Agriculture Obihiro University of Agriculture and Veterinary Medicine  Obihiro Hokkaido Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yusuke","family":"Sugimoto","sequence":"additional","affiliation":[{"name":"Ajinomoto Co., Inc.  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