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Eighteen mature (4\u20136\u2009years old) rams (52.8\u2009\u00b1\u20092.6\u2009kg) were divided into three groups. The control (C) group was fed oat hay ad\u00a0libitum with 700\u2009g of concentrate and the other two groups were fed the same diet supplemented with AES at 1 and 2\u2009mg\/kg body weight (AES1 and AES2, respectively). Ram sperm was collected with an artificial vagina (2\u2009\u00d7\u20092\u2009days\/week) to evaluate sperm production and quality, antioxidant activity, the adenosine triphosphate (ATP) and calcium concentrations. Sexual behaviour and plasma testosterone concentrations were also investigated. The administration of AES improved sexual behaviour (the duration of contact and the number of lateral approaches). The addition of AES also improved individual spermatozoa motility (C: 71.7%\u2009\u00b1\u20096.3%; AES1: 78.3%\u2009\u00b1\u20094.9%; AES2: 83.8%\u2009\u00b1\u20094.4%), the sperm concentration (C: 5.6\u2009\u00b1\u20090.36; AES1: 6.4\u2009\u00b1\u20090.81; AES2: 6.7\u2009\u00b1\u20090.52 \u00d7\u200910<jats:sup>9<\/jats:sup> spermatozoa\/mL), the ATP ratio (C: 1\u2009\u00b1\u20090.08; AES1: 2.1\u2009\u00b1\u20090.08; AES2: 3.3\u2009\u00b1\u20090.08) and the calcium concentration (C: 5.6\u2009\u00b1\u20090.24; AES1: 7.7\u2009\u00b1\u20090.21; AES2: 8.1\u2009\u00b1\u20090.24\u2009mmol\/L). AES treatment decreased the percentage of abnormal sperm (C: 18.5%\u2009\u00b1\u20091.2%; AES1: 16.2%\u2009\u00b1\u20091.1%; AES2: 14.8%\u2009\u00b1\u20090.94%) and DNA damage (C: 62%; AES1: 27%; AES2: 33%) and was associated with elevated seminal fluid antioxidant activity (C: 22\u2009\u00b1\u20090.27; AES1: 27.1\u2009\u00b1\u20091.08 and AES2: 27.5\u2009\u00b1\u20090.36\u2009mmol Trolox equivalents\/L) and plasma testosterone (C: 8.3\u2009\u00b1\u20090.7; AES1: 11.7\u2009\u00b1\u20090.4; AES2: 15\u2009\u00b1\u20090.7\u2009ng\/L). In conclusion, our study suggests that <jats:italic>S. atropurpurea<\/jats:italic> may be potentially useful to enhance libido and sperm production and quality in ram.<\/jats:p>","DOI":"10.1111\/rda.14569","type":"journal-article","created":{"date-parts":[[2024,5,8]],"date-time":"2024-05-08T06:29:12Z","timestamp":1715149752000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Oral supplementation with <i>Scabiosa artropurperea<\/i> L. aqueous extract improves the in\u00a0vivo sexual behaviour, sperm quality and androgen level in rams"],"prefix":"10.1111","volume":"59","author":[{"given":"Wijden","family":"Niama","sequence":"first","affiliation":[{"name":"Universit\u00e9 de Jendouba, Ecole Sup\u00e9rieure d'Agriculture du Kef, Laboratoire d'Appui \u00e0 la Durabilit\u00e9 des Syst\u00e8mes de Production Agricole dans la R\u00e9gion du Nord\u2010Ouest ESAK  Kef Tunisia"},{"name":"CNRS, IFCE, INRAE, Universit\u00e9 de 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