{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T00:08:11Z","timestamp":1769731691451,"version":"3.49.0"},"reference-count":42,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,5,7]],"date-time":"2024-05-07T00:00:00Z","timestamp":1715040000000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100010719","name":"Pakistan Agricultural Research Council","doi-asserted-by":"publisher","award":["AS\u2010035"],"award-info":[{"award-number":["AS\u2010035"]}],"id":[{"id":"10.13039\/501100010719","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study evaluates factors influencing pregnancy rates per artificial insemination (P\/AI) and pregnancy loss in Lohi ewes undergoing laparoscopic AI with frozen\u2013thawed semen under sub\u2010tropical conditions. Data from three experiments comprising ewes (<jats:italic>n<\/jats:italic>\u2009=\u2009358) of mixed parity (nulliparous; NP and parous; P), various body condition score (BCS) and assigned to long\u2010term (LTP, 11\u2009days) and short\u2010term (STP, 5\u2009days) oestrus synchronization regimen across high breeding season (HBS) and low breeding season (LBS) were analysed. Laparoscopic insemination was conducted 54\u2009h post\u2010sponge removal. Pregnancy diagnosis and loss were evaluated on days 35 and 90 post\u2010insemination via ultrasonography. Results showed parity significantly influenced P\/AI, with nulliparous ewes achieving higher pregnancy ratios than parous ewes (<jats:italic>p\u2009=<\/jats:italic>\u2009.001). BCS significantly influenced P\/AI (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05), with a quadratic relationship observed between BCS and season (BCS*BCS*Season; <jats:italic>p<\/jats:italic>\u2009=\u2009.07). Progestin treatment did not significantly influence the ratio of pregnant ewes (<jats:italic>p<\/jats:italic>\u2009=\u2009.07). Pregnancy losses were significantly higher during LBS than HBS (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05), irrespective of progestin treatment. In conclusion, parity and BCS significantly influenced P\/AI, with BCS demonstrating a quadratic association with season. Ewes bred during LBS experienced higher pregnancy losses than HBS, irrespective of progestin treatment.<\/jats:p>","DOI":"10.1111\/rda.14579","type":"journal-article","created":{"date-parts":[[2024,5,8]],"date-time":"2024-05-08T06:45:50Z","timestamp":1715150750000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Factors influencing pregnancy rate and loss after laparoscopic artificial insemination with frozen\u2013thawed semen in Lohi sheep under sub\u2010tropical conditions"],"prefix":"10.1111","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4624-1531","authenticated-orcid":false,"given":"Muhammad Tayyab","family":"Khan","sequence":"first","affiliation":[{"name":"Department of Theriogenology, Faculty of Veterinary Science University of Veterinary and Animal Sciences  Lahore Pakistan"},{"name":"Department of Clinical Sciences, Faculty of Veterinary Science Bahauddin Zakariya University  Multan Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1992-7941","authenticated-orcid":false,"given":"Ejaz","family":"Ahmad","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, Faculty of Veterinary Science Bahauddin Zakariya University  Multan Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ali","family":"Husnain","sequence":"additional","affiliation":[{"name":"Department of Theriogenology, Faculty of Veterinary Science University of Veterinary and Animal Sciences  Lahore Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad Rizwan","family":"Yousaf","sequence":"additional","affiliation":[{"name":"Department of Theriogenology, Faculty of Veterinary Science University of Veterinary and Animal Sciences  Lahore Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad","family":"Oneeb","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Veterinary Science University of Veterinary and Animal Sciences  Lahore Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tanveer","family":"Ahmad","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, Faculty of Veterinary Science Bahauddin Zakariya University  Multan Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2432-5753","authenticated-orcid":false,"given":"Muhammad Irfan\u2010ur\u2010Rehman","family":"Khan","sequence":"additional","affiliation":[{"name":"Department of Theriogenology, Faculty of Veterinary Science University of Veterinary and Animal Sciences  Lahore 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