{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T05:26:43Z","timestamp":1768541203247,"version":"3.49.0"},"reference-count":36,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,5,17]],"date-time":"2024-05-17T00:00:00Z","timestamp":1715904000000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The current study aimed to explore the molecular mechanism by which the cholecystokinin (CCK)\u2010mediated CCKAR and CCKBR, as well as the molecular mechanisms of CCK\u2010mediated insulin signalling pathway, regulate oestrogen in the granulosa cells. Also, the expression of CCK in ovaries, uterus, hypothalamus and pituitary gland was investigated in <jats:italic>Camelus bactrianus<\/jats:italic>. Ovaries, uterus, hypothalamus and pituitary gland were collected from six, three before ovulation (control) and three after ovulation, slaughtered <jats:italic>Camelus bactrianus<\/jats:italic>. Ovulation was induced by IM injection of seminal plasma before slaughtering in the ovulated group. The results showed that there were differences in the transcription and protein levels of CCK in various tissues before and after ovulation (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05, <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.01). After transfection with p\u2010IRES2\u2010EGFP\u2010CCK, the mRNA and protein levels of CCK, CCKAR, CCKBR and ER in follicular granulosa cells were significantly upregulated (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05, <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.01), and the content of E<jats:sub>2<\/jats:sub> was significantly upregulated (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.01); On the contrary, after transfection with si\u2010CCK, the mRNA and protein levels of CCK, CCKAR, CCKBR and ER in follicular granulosa cells were significantly downregulated (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05, <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.01), and the content of E<jats:sub>2<\/jats:sub> was significantly downregulated (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.01). Regulating CCK can affect the mRNA levels of <jats:italic>INS<\/jats:italic>, <jats:italic>INSR<\/jats:italic>, <jats:italic>IGF<\/jats:italic> and <jats:italic>IGF\u2010R<\/jats:italic>. In summary, regulating the expression level of CCK can activate insulin\u2010related signalling pathways by CCKR, thereby regulating the steroidogenic activity of granulosa cells.<\/jats:p>","DOI":"10.1111\/rda.14586","type":"journal-article","created":{"date-parts":[[2024,5,17]],"date-time":"2024-05-17T09:04:17Z","timestamp":1715936657000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Cholecystokinin\u2010mediated effect of insulin pathway on the steroidogenic activity of follicular granulosa cells in <i>Camelus bactrianus<\/i>: In\u00a0vitro study"],"prefix":"10.1111","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-1773-3357","authenticated-orcid":false,"given":"Lusha","family":"Guo","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine Gansu Agricultural University  Lanzhou China"},{"name":"Gansu Key Laboratory of Animal Reproductive Physiology and Reproductive  Lanzhou 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