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As a result of the pregnancy examination, the cows were divided into two main groups according to whether they were pregnant. The results showed that leucyl\/cystinyl aminopeptidase (LNPEP) concentration was significantly decreased B\u2010AI and Secreted frizzled\u2010related proteins (SFRP\u20103), Vascular Endothelial Growth Factor (VEGF) and LNPEP levels were significantly decreased on day of AI, while PRL level was increased, and these data have prognostic significance as early indicator of the risk of potentially failed pregnancy. Additionally, a significant decrease in LNPEP, SFRP3, and VEGF levels, along with an increase in PRL levels was also observed in A\u2010AI. These results suggest that these biomarkers can be used as a screening test to monitor the course of pregnancy. There were no significant differences in serum levels of Insulin\u2010Like Growth Factor 2 (IGF\u20102), Tissue inhibitors of metalloproteinases (TIMP\u20101), angiopoietin (ANG), Endoglin (ENG), Fibroblast growth factor (FGF), Inhibine\u2010A (INH\u2010A) and Transforming growth factors\u2010\u03b21 (TGF\u2010\u03b21) between the evaluated periods neither unsuccessful nor the successful pregnancy groups. This is the first study reporting that the maternal serum levels of LNPEP, SFRP3, VEGF, and PRL have important roles in pregnancy success and may indicate whether insemination outcome will be successful B\u2010AI and predict the risk of unsuccessful pregnancy after AI in dairy cattle. The increase in such studies will allow the development of more specific, practical, and applicable markers.<\/jats:p>","DOI":"10.1111\/rda.14587","type":"journal-article","created":{"date-parts":[[2024,5,30]],"date-time":"2024-05-30T06:59:04Z","timestamp":1717052344000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>Early prognostic markers<\/scp> to <scp>predict unsuccessful pregnancy<\/scp> in <scp>dairy cattle<\/scp>"],"prefix":"10.1111","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4585-3712","authenticated-orcid":false,"given":"Beran","family":"Yokus","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Faculty of Veterinary Medicine University of Dicle  Diyarbakir 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