{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:45:06Z","timestamp":1753879506041,"version":"3.41.2"},"reference-count":9,"publisher":"Wiley","issue":"S3","license":[{"start":{"date-parts":[[2024,10,13]],"date-time":"2024-10-13T00:00:00Z","timestamp":1728777600000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100014440","name":"Ministerio de Ciencia, Innovaci\u00f3n y Universidades","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002809","name":"Generalitat de Catalunya","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Goat production is affected by reproductive seasonality. In\u00a0vitro embryo production (IVEP) could overcome this effect. This study aimed to evaluate the impact of the season of semen collection\/freezing on IVEP of prepubertal goat oocytes and on sperm quality and functionality concerning capacitation. Semen from six fertile bucks was collected, pooled and cryopreserved in spring and autumn and used for IVEP of oocytes recovered during the breeding season. Oocytes were IVM in TCM\u2010199 with hormones, EGF and cysteamine; fertilized and cultured in BO\u2010IVF and BO\u2010IVC media (IVF Bioscience, UK). Semen samples were assessed at 0 and 3\u2009h after culture in capacitating (BO\u2010IVF, CAP) and non\u2010capacitating conditions for sperm plasma membrane and acrosome integrity, mitochondrial membrane potential (MMP), intracellular calcium and plasma membrane lipid disorder. Blastocyst production was higher with spring sperm compared to autumn (12.0% vs. 2.1%, respectively; <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). After CAP, acrosome reaction and intracellular calcium were higher (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05) in spring than autumn sperm. No differences were found in other sperm parameters. In conclusion, seasonal variations in the IVEP of prepubertal goats could be linked to differences in sperm ability to undergo in\u00a0vitro capacitation.<\/jats:p>","DOI":"10.1111\/rda.14594","type":"journal-article","created":{"date-parts":[[2024,10,14]],"date-time":"2024-10-14T01:31:43Z","timestamp":1728869503000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Impact of semen cryopreservation season on in\u00a0vitro embryo production of prepubertal goat oocytes"],"prefix":"10.1111","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3069-435X","authenticated-orcid":false,"given":"M\u00f2nica","family":"Ferrer\u2010Roda","sequence":"first","affiliation":[{"name":"Department of Animal and Food Science, Veterinary Faculty Autonomous University of Barcelona  Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0878-7819","authenticated-orcid":false,"given":"Jaime","family":"Catal\u00e1n","sequence":"additional","affiliation":[{"name":"Biotechnology of Animal and Human Reproduction (TechnoSperm), Institute of Food and Agricultural Technology University of Girona  Girona Spain"},{"name":"Unit of Cell Biology, Department of Biology, Faculty of Sciences University of Girona  Girona Spain"},{"name":"Department of Animal Medicine and Surgery, Faculty of Veterinary Sciences Autonomous University of Barcelona  Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1954-2868","authenticated-orcid":false,"given":"Dolors","family":"Izquierdo","sequence":"additional","affiliation":[{"name":"Department of Animal and Food Science, Veterinary Faculty Autonomous University of Barcelona  Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7730-290X","authenticated-orcid":false,"given":"Maria Emilia Franco","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Department of Animal and Food Science, Veterinary Faculty Autonomous University of Barcelona  Barcelona Spain"},{"name":"School of Agricultural and Veterinarian Sciences S\u00e3o Paulo State University  Sao Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3653-654X","authenticated-orcid":false,"given":"Maria\u2010Teresa","family":"Paramio","sequence":"additional","affiliation":[{"name":"Department of Animal and Food Science, Veterinary Faculty Autonomous University of Barcelona  Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,10,13]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smallrumres.2018.10.003"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox13030322"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.12274"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smallrumres.2018.11.026"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani11123452"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2013.08.005"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2020.01.046"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0221663"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD20206"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.14594","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,17]],"date-time":"2025-04-17T05:38:15Z","timestamp":1744868295000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.14594"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10]]},"references-count":9,"journal-issue":{"issue":"S3","published-print":{"date-parts":[[2024,10]]}},"alternative-id":["10.1111\/rda.14594"],"URL":"https:\/\/doi.org\/10.1111\/rda.14594","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"type":"print","value":"0936-6768"},{"type":"electronic","value":"1439-0531"}],"subject":[],"published":{"date-parts":[[2024,10]]},"assertion":[{"value":"2024-03-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-10-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e14594"}}