{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T09:24:08Z","timestamp":1792488248943,"version":"4.5.0"},"reference-count":55,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T00:00:00Z","timestamp":1718582400000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003787","name":"Natural Science Foundation of Hebei Province","doi-asserted-by":"publisher","award":["H2013406115"],"award-info":[{"award-number":["H2013406115"]}],"id":[{"id":"10.13039\/501100003787","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Our previous research has shown that melatonin (MLT) can reduce cryopreserved ovarian damage in mice. Yet, the molecular mechanism of MLT protection is still unclear. Some studies have shown that melatonin receptor 1 (MT1) is very important for animal reproductive system. To evaluate whether MLT exerts its protective effect on cryopreserved mice ovarian tissue via MT1, we added antagonist of MT1\/MT2 (Luzindor) or antagonist of MT2 (4P\u2010PDOT) to the freezing solution, followed by cryopreservation and thawing of ovarian tissue. The levels of total superoxide dismutase (T\u2010SOD), catalase (CAT), nitric oxide (NO) and malondialdehyde (MDA) were detected. Besides, by using RT\u2010PCR and Western blotting, the expression of Bcl\u20102, Bax and Nrf2\/HO\u20101 signalling pathway\u2010related proteins was detected. These findings demonstrated that compared with the melatonin group, the addition of Luzindor increased apoptosis, NO and MDA activities, decreased CAT and T\u2010SOD activities and inhibited Nrf2\/HO\u20101 signalling pathway. In conclusion, melatonin can play a protective role in cryopreserved ovarian tissue of mice through MT1 receptor.<\/jats:p>","DOI":"10.1111\/rda.14598","type":"journal-article","created":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T04:49:30Z","timestamp":1718599770000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Melatonin activates Nrf2\/\n                    <scp>HO<\/scp>\n                    \u20101 signalling pathway to antagonizes oxidative stress\u2010induced injury via melatonin receptor 1 (\n                    <scp>MT1<\/scp>\n                    ) in cryopreserved mice ovarian tissue"],"prefix":"10.1111","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-1258-1575","authenticated-orcid":false,"given":"Chen Yang","family":"Wei","sequence":"first","affiliation":[{"name":"Faculty of Graduate Studies Chengde Medical University  Chengde Hebei China"},{"name":"Department of Human 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