{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T05:27:57Z","timestamp":1772602077512,"version":"3.50.1"},"reference-count":13,"publisher":"Wiley","issue":"S3","license":[{"start":{"date-parts":[[2024,10,13]],"date-time":"2024-10-13T00:00:00Z","timestamp":1728777600000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\u03b2\u2010nerve growth factor (\u03b2NGF) plays a crucial role in reproductive physiology and sperm quality. Enzymatic activity of seminal plasma and vaginal fluids reduces available \u03b2NGF and it has been demonstrated that chitosan microspheres could protect rr\u03b2NGF from degradation. This study examined the effects of microencapsulated rrbNGF with chitosan on rabbit sperm viability, motility and capacitation status. Results showed that 0.5 and 1\u2009\u03bcg\/mL of microencapsulated rr\u03b2NGF, as well as free rr\u03b2NGF or empty microspheres, did not adversely affect sperm viability or total motility after 2\u2009h of incubation. However, the highest progressivity kinetic parameters were observed with 1\u2009\u03bcg\/mL free rr\u03b2NGF, while the highest curvilinear velocity (VCL) occurred with 0.5\u2009\u03bcg\/mL microencapsulated rr\u03b2NGF. Empty chitosan microspheres did not induce acrosome reaction (AR), but both concentrations of free and rr\u03b2NGFch favoured AR during in\u00a0vitro incubation. The study suggests that using chitosan spheres did not show any adverse effects on sperm traits, unlike free r\u03b2NGF and rr\u03b2NGFch promoted capacitation and AR. Further research is needed to explore the potential of rr\u03b2NGFch in modifying in\u00a0vitro capacitation and inducing ovulation during artificial insemination.<\/jats:p>","DOI":"10.1111\/rda.14636","type":"journal-article","created":{"date-parts":[[2024,10,14]],"date-time":"2024-10-14T01:26:29Z","timestamp":1728869189000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Influence of free and microencapsulated recombinant rabbit nerve growth factor with chitosan on rabbit sperm quality parameters"],"prefix":"10.1111","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0455-3409","authenticated-orcid":false,"given":"Silvia","family":"Gimeno\u2010Martos","sequence":"first","affiliation":[{"name":"Dpto. Fisiolog\u00eda, Facultad de Veterinaria Universidad Complutense de Madrid  Madrid Spain"},{"name":"Facultad de Veterinaria Universidad de Zaragoza  Zaragoza Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luigia","family":"Bosa","sequence":"additional","affiliation":[{"name":"Dpto Agricultura, Medio Ambiente y Alimentaria Universidad de Perugia  Perugia Italy"},{"name":"Dpto Producci\u00f3n Agraria E.T.S.I. A.A.B., Universidad Polit\u00e9cnica de Madrid  Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pedro Luis","family":"Lorenzo","sequence":"additional","affiliation":[{"name":"Dpto. Fisiolog\u00eda, Facultad de Veterinaria Universidad Complutense de Madrid  Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda","family":"Arias\u2010Alvarez","sequence":"additional","affiliation":[{"name":"Dpto. Producci\u00f3n Animal, Facultad de Veterinaria Universidad Complutense de Madrid  Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cesare","family":"Castellini","sequence":"additional","affiliation":[{"name":"Dpto Agricultura, Medio Ambiente y Alimentaria Universidad de Perugia  Perugia Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4418-0157","authenticated-orcid":false,"given":"Pilar","family":"Garc\u00eda\u2010Rebollar","sequence":"additional","affiliation":[{"name":"Dpto Producci\u00f3n Agraria E.T.S.I. A.A.B., Universidad Polit\u00e9cnica de Madrid  Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3393-8906","authenticated-orcid":false,"given":"Rosa Mar\u00eda","family":"Garc\u00eda\u2010Garc\u00eda","sequence":"additional","affiliation":[{"name":"Dpto. Fisiolog\u00eda, Facultad de Veterinaria Universidad Complutense de Madrid  Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,10,13]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.2174\/187231309788166415"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD17525"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2020.04.042"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12958\u2010019\u20100533\u20104"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2013.05.002"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod63.6.1617"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1530\/rep\u201017\u20100177"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.09\u2010219N"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1061"},{"key":"e_1_2_9_11_1","doi-asserted-by":"crossref","unstructured":"Quiroga A. C. Gimeno\u2010Martos S. Lorenzo P. L. Arias\u2010\u00c1lvarez M. Rebollar P. G. &Garc\u00eda\u2010Garc\u00eda R. M.(2023).Microencapsulated rrbNGF as an Alternative Ovulation Induction Method in Rabbits[Preprint]. In Review.https:\/\/doi.org\/10.21203\/rs.3.rs\u20103125067\/v1","DOI":"10.21203\/rs.3.rs-3125067\/v1"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0223091"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2020.08.003"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2014.02.003"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.14636","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,17]],"date-time":"2025-04-17T05:38:24Z","timestamp":1744868304000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.14636"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10]]},"references-count":13,"journal-issue":{"issue":"S3","published-print":{"date-parts":[[2024,10]]}},"alternative-id":["10.1111\/rda.14636"],"URL":"https:\/\/doi.org\/10.1111\/rda.14636","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10]]},"assertion":[{"value":"2024-03-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-05-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-10-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e14636"}}