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The research evaluated the impact of three different in\u00a0vitro culture media on blastocyst formation. Oocytes and spermatozoa were collected and processed, followed by in\u00a0vitro fertilization and culture. Results returned a similar blastocyst rate (ANOVA, <jats:italic>p<\/jats:italic>\u2009&gt;\u2009.05), over 16% across all groups. While demonstrating the potential of these techniques, further investigations are warranted to evaluate embryo quality to refine optimal protocols and their applicability in felid conservation efforts.<\/jats:p>","DOI":"10.1111\/rda.14645","type":"journal-article","created":{"date-parts":[[2024,10,14]],"date-time":"2024-10-14T01:24:31Z","timestamp":1728869071000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Enhancing felid conservation: Exploring the impact of in\u00a0vitro culture media on domestic cat blastocyst production"],"prefix":"10.1111","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6140-9238","authenticated-orcid":false,"given":"Ana","family":"Munoz\u2010Maceda","sequence":"first","affiliation":[{"name":"Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine Complutense University of Madrid  Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-9537-6055","authenticated-orcid":false,"given":"Andrea","family":"Priego\u2010Gonzalez","sequence":"additional","affiliation":[{"name":"Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine Complutense University of Madrid  Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carolina","family":"N\u00fa\u00f1ez\u2010Puente","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction National Institute for Agricultural and Food Research and Technology (INIA\u2010CSIC)  Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6813-3940","authenticated-orcid":false,"given":"Dimitrios","family":"Rizos","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction National Institute for Agricultural and Food Research and Technology (INIA\u2010CSIC)  Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8710-6454","authenticated-orcid":false,"given":"Joaqu\u00edn","family":"Cerdeira\u2010Lozano","sequence":"additional","affiliation":[{"name":"Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine Complutense University of Madrid  Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3724-9470","authenticated-orcid":false,"given":"Ana","family":"Sanchez\u2010Rodriguez","sequence":"additional","affiliation":[{"name":"Department of Biodiversity and Evolutionary Biology National Museum of Natural Sciences (CSIC)  Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7545-4248","authenticated-orcid":false,"given":"Eduardo R. 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