{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T19:06:44Z","timestamp":1777576004509,"version":"3.51.4"},"reference-count":42,"publisher":"Wiley","issue":"S3","license":[{"start":{"date-parts":[[2024,10,13]],"date-time":"2024-10-13T00:00:00Z","timestamp":1728777600000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007801","name":"Fundaci\u00f3n S\u00e9neca","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007801","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Nanotechnology and its applications have advanced significantly in recent decades, contributing to various fields, including reproduction. This study introduces a novel method to label porcine oocytes with nanoparticles (NPs) bound to oviductin (OVGP1, Ov) for use in Assisted Reproductive Technologies (ARTs). Despite promising developments, concerns about NP toxicity in gametes necessitate thorough investigation. This research aims to assess the impact of functionalized NPs (NPOv) on sperm functionality. Boar sperm were co\u2010incubated with NPOv for 0, 0.5 and 1\u2009h in two media: BTS (semen dilution and conservation) and TALP (sperm capacitation and in\u00a0vitro fertilization\u2010IVF). Sperm quality parameters (viability, motility and kinematics) showed no significant differences in TALP medium (<jats:italic>p<\/jats:italic>\u2009&gt;\u2009.05). In BTS, although\u00a0some kinetic parameters were altered, motility, progressive motility and viability remained unaffected (<jats:italic>p<\/jats:italic>\u2009&gt;\u2009.05). Additionally, NPs presence on the zona pellucida (ZP) of oocytes did not affect sperm attachment (<jats:italic>p<\/jats:italic>\u2009&gt;\u2009.05). In conclusion, in\u00a0vitro exposure of boar sperm to OVGP1\u2010functionalized NPs in IVF medium or attached to the ZP surface of matured oocytes does not impair sperm functionality, including their binding ability to the ZP.<\/jats:p>","DOI":"10.1111\/rda.14654","type":"journal-article","created":{"date-parts":[[2024,10,14]],"date-time":"2024-10-14T01:23:18Z","timestamp":1728868998000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["In vitro exposure of porcine sperm to functionalized superparamagnetic nanoparticles"],"prefix":"10.1111","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7557-3872","authenticated-orcid":false,"given":"Gabriela","family":"Garrappa","sequence":"first","affiliation":[{"name":"Departamento de Fisiolog\u00eda, Facultad de Veterinaria, Campus de Excelencia Mare Nostrum Universidad de Murcia  Murcia Spain"},{"name":"Instituto Nacional de Tecnolog\u00eda Agropecuaria (INTA)  Rafaela Santa Fe Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristina","family":"Mart\u00ednez\u2010L\u00f3pez","sequence":"additional","affiliation":[{"name":"Departamento de Fisiolog\u00eda, Facultad de Veterinaria, Campus de Excelencia Mare Nostrum Universidad de Murcia  Murcia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda","family":"Jim\u00e9nez\u2010Movilla","sequence":"additional","affiliation":[{"name":"Instituto Murciano de Investigaci\u00f3n Biosanitaria (IMIB\u2010Arrixaca)  Murcia Spain"},{"name":"Departamento de Biolog\u00eda Celular e Histolog\u00eda, Facultad de Medicina y Enfermer\u00eda, Campus de Excelencia Mare Nostrum Universidad de Murcia  Murcia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7665-3858","authenticated-orcid":false,"given":"Francisco A.","family":"Garc\u00eda\u2010V\u00e1zquez","sequence":"additional","affiliation":[{"name":"Departamento de Fisiolog\u00eda, Facultad de Veterinaria, Campus de Excelencia Mare Nostrum Universidad de Murcia  Murcia Spain"},{"name":"Instituto Murciano de Investigaci\u00f3n Biosanitaria (IMIB\u2010Arrixaca)  Murcia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,10,13]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep32556"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.2147\/NSA.S99986"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2017.02.022"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nano.2014.01.001"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfi256"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1080\/02656736.2017.1341646"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.23670"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.201303947"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.livsci.2017.09.024"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40104-018-0307-4"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijvsm.2018.09.001"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.22831"},{"issue":"2","key":"e_1_2_9_14_1","article-title":"Lectin\u2010functionalized magnetic iron oxide nanoparticles for reproductive improvement","volume":"3","author":"Feugang J. 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