{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:45:15Z","timestamp":1753879515841,"version":"3.41.2"},"reference-count":31,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,7,21]],"date-time":"2024-07-21T00:00:00Z","timestamp":1721520000000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Animal reproduction biotechniques are important tools for the technological advancement of livestock, as they allow the selection of the reproductive potential of superior quality females and males; however, infectious diseases that have a predilection for the reproductive system can be a hindrance for the use of these technologies. Therefore, the present study aimed to detect <jats:italic>Brucella<\/jats:italic> spp. in the ovarian follicular fluid of brucellosis\u2010positive bovine cows. A total of 47 bovine ovarian follicular fluid aspirates from cows, positive in tests for brucellosis and from <jats:italic>Brucella<\/jats:italic>\u2010positive herd, were submitted to PCR. The primers used in the PCR were specific to the genus <jats:italic>Brucella<\/jats:italic> (<jats:italic>bcsp31<\/jats:italic> gene). All 47 bovine aspirates were negative for <jats:italic>Brucella<\/jats:italic> spp. 0.00% (95% CI: 0.00\u20134.00%). Our results demonstrated that <jats:italic>Brucella<\/jats:italic> spp. was absent in the ovarian follicular fluid from seropositive cows, which indicates that <jats:italic>Brucella<\/jats:italic> spp.\u2010infected cows could be used for reproductive biotechnologies carried out with follicular aspirates. Future studies are needed to more precisely evaluate the feasibility and safety of using these oocytes from brucellosis\u2010seropositive cows to transfer embryos to heifers\/cows not infected by <jats:italic>Brucella<\/jats:italic>, aiming to produce calves free of the infection.<\/jats:p>","DOI":"10.1111\/rda.14672","type":"journal-article","created":{"date-parts":[[2024,7,22]],"date-time":"2024-07-22T05:55:00Z","timestamp":1721627700000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Is it safe to use ovarian follicular fluid from cows seropositive for brucellosis in the production of in\u00a0vitro embryos?"],"prefix":"10.1111","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0195-7926","authenticated-orcid":false,"given":"Rafaella Silva","family":"Andrade","sequence":"first","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria, Faculdade de Zootecnia e Medicina Veterin\u00e1ria Universidade Federal de Lavras  Lavras Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8279-7169","authenticated-orcid":false,"given":"Dirc\u00e9ia Aparecida da Costa","family":"Cust\u00f3dio","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria, Faculdade de Zootecnia e Medicina Veterin\u00e1ria Universidade Federal de Lavras  Lavras Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-0234-3097","authenticated-orcid":false,"given":"Leonardo","family":"Silva\u00a0Fernandes\u00a0do\u00a0Vale","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria, Faculdade de Zootecnia e Medicina Veterin\u00e1ria Universidade Federal de Lavras  Lavras Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1918-4658","authenticated-orcid":false,"given":"\u00c9rika Aparecida","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria, Faculdade de Zootecnia e Medicina Veterin\u00e1ria Universidade Federal de Lavras  Lavras Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9325-5728","authenticated-orcid":false,"given":"Andrey Pereira","family":"Lage","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria Preventiva, Escola de Veterin\u00e1ria Universidade Federal de Minas Gerais  Belo Horizonte Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2753-1296","authenticated-orcid":false,"given":"Elaine Maria Seles","family":"Dorneles","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria, Faculdade de Zootecnia e Medicina Veterin\u00e1ria Universidade Federal de Lavras  Lavras Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,7,21]]},"reference":[{"key":"e_1_2_10_2_1","first-page":"1022","article-title":"Detection of Brucella abortus during in\u00a0vitro bovine embryo production","volume":"105","author":"Akter A.","year":"2020","journal-title":"Bangladesh Journal of Livestock Research"},{"key":"e_1_2_10_3_1","first-page":"271","article-title":"Detection of Brucella melitensis and Brucella abortus by DNA amplification","volume":"95","author":"Baily G. 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