{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,8]],"date-time":"2025-12-08T22:44:08Z","timestamp":1765233848220,"version":"3.41.2"},"reference-count":31,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,7,31]],"date-time":"2024-07-31T00:00:00Z","timestamp":1722384000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100012547","name":"Natural Science Foundation of Guangxi Zhuang Autonomous Region","doi-asserted-by":"publisher","award":["2021GXNSFAA196021","2020GXNSFAA159002"],"award-info":[{"award-number":["2021GXNSFAA196021","2020GXNSFAA159002"]}],"id":[{"id":"10.13039\/100012547","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study used the brilliant cresyl blue (BCB) staining method to group buffalo oocytes (BCB+ and BCB\u2212) and perform in\u00a0vitro maturation, in\u00a0vitro fertilization and embryo culture. At the same time, molecular biology techniques were used to detect gap junction protein expression and oxidative stress\u2010related indicators to explore the molecular mechanism of BCB staining to predict oocyte developmental potential. The techniques of buffalo oocytes to analyse their developmental potential and used immunofluorescence staining to detect the expression level of CX43 protein, DCFH\u2010DA probe staining to detect ROS levels and qPCR to detect the expression levels of the antioxidant\u2010related genes SOD2 and GPX1. Our results showed that the in\u00a0vitro maturation rate, embryo cleavage rate and blastocyst rate of buffalo oocytes in the BCB+ group were significantly higher than those in the BCB\u2212 group and the control group (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). The expression level of CX43 protein in the BCB+ group was higher than that in the BCB\u2212 group both before and after maturation (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). The intensity of ROS in the BCB+ group was significantly lower than that in the BCB\u2212 group (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05), and the expression levels of the antioxidant\u2010related genes SOD2 and GPX1 in the BCB+ group were significantly higher than those in the BCB\u2212 group (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). Brilliant cresyl blue staining could effectively predict the developmental potential of buffalo oocytes. The results of BCB staining were positively correlated with the expression of gap junction protein and antioxidant\u2010related genes and negatively correlated with the reactive oxygen species level, suggesting that the mechanism of BCB staining in predicting the developmental potential of buffalo oocytes might be closely related to antioxidant activity.<\/jats:p>","DOI":"10.1111\/rda.14673","type":"journal-article","created":{"date-parts":[[2024,8,1]],"date-time":"2024-08-01T04:46:04Z","timestamp":1722487564000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["CX43 and oxidative stress are the targets of BCB staining to predict the developmental potential of buffalo oocytes"],"prefix":"10.1111","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-8886-7492","authenticated-orcid":false,"given":"MengQi","family":"Li","sequence":"first","affiliation":[{"name":"Guangxi Key Laboratory of Buffalo Genetics, 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