{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T14:26:13Z","timestamp":1770474373524,"version":"3.49.0"},"reference-count":42,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,7,29]],"date-time":"2024-07-29T00:00:00Z","timestamp":1722211200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The reproductive efficiency of dromedary camels is hindered by challenges such as early embryonic mortality, which may be attributed to a lack of synchronization between conceptus signalling and uterine receptivity. Understanding the intricate biological processes involved in feto\u2010maternal interactions during implantation is crucial to address these limitations. Osteopontin (OPN) is a protein involved in cell signalling and adhesion, playing a crucial role in embryonic implantation. Previous studies have shown the presence of OPN in the uterine endometrium of various mammalian species including dromedary camels. However, the expression pattern of OPN in dromedary conceptuses remains unexplored. Thus, the current study aimed, for the first time, to investigate the temporospatial expression of OPN in dromedary conceptuses during the peri\u2010implantation period at Days 8, 10, and 12 of pregnancy. Twelve conceptuses were recovered non\u2010surgically from pregnant females on Days 8, 10, 12 of pregnancy. Quantitative real\u2010time PCR (qrt\u2010PCR), immunohistochemistry (IHC), and immunofluorescence (IF) were employed for analysis of the expression of OPN mRNA and protein. The results revealed significant increases in both OPN mRNA and protein expression started on Day 10 and peaked at Day 12 of pregnancy. Immuno\u2010localization confirmed the presence of OPN protein in the trophectoderm and endoderm of dromedary conceptuses. In conclusion, the expression and localization of OPN during the peri\u2010implantation period in dromedary conceptuses imply its involvement as a crucial reproductive factor and its upregulation during this period, with a pronounced increase close to attachment time (Day 12 of pregnancy) further supports its role in embryo adhesion, implantation, and placentation.<\/jats:p>","DOI":"10.1111\/rda.14694","type":"journal-article","created":{"date-parts":[[2024,7,30]],"date-time":"2024-07-30T06:34:31Z","timestamp":1722321271000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Osteopontin expression in dromedary camel's conceptuses during the peri\u2010implantation period"],"prefix":"10.1111","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7058-3649","authenticated-orcid":false,"given":"Mohammed Salem","family":"Moqbel","sequence":"first","affiliation":[{"name":"Department of Anatomy, College of Veterinary Medicine King Faisal University  Al\u2010Ahsa Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdulraman Khalid","family":"Alhaider","sequence":"additional","affiliation":[{"name":"Department of Clinical Studies, College of Veterinary Medicine King Faisal University  Al\u2010Ahsa Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Faisal","family":"Almathen","sequence":"additional","affiliation":[{"name":"Department of Veterinary Public Health, College of Veterinary Medicine King Faisal University  Al\u2010Ahsa Saudi Arabia"},{"name":"Camel Research Center King Faisal University  Al\u2010Ahsa Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nidhal Neji Ben","family":"Amor","sequence":"additional","affiliation":[{"name":"Department of Veterinary Public Health, College of Veterinary Medicine King Faisal University  Al\u2010Ahsa Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saeed Yaseen","family":"Al\u2010Ramadan","sequence":"additional","affiliation":[{"name":"Department of Anatomy, College of Veterinary Medicine King Faisal University  Al\u2010Ahsa Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,7,29]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2016.11.033"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.101.001347"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells8050432"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.12.13.2073"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.141"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP\u201008\u20100304"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1159\/000066969"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.18548\/aspe\/0005.13"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/8.9.871"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1472\u20106483(10)60146\u20100"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.137.5.8612515"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.109.078600"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP\u201017\u20100043"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod66.3.718"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.285.5430.1028"},{"key":"e_1_2_10_17_1","unstructured":"Hermens A.(2012).Expression and localization of osteopontin in the equine endometrium and conceptus during the cycle and peri\u2010implantation period. 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