{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T13:15:08Z","timestamp":1773666908116,"version":"3.50.1"},"reference-count":47,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,7,29]],"date-time":"2024-07-29T00:00:00Z","timestamp":1722211200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The objective of the present study was to investigate the association among the largest follicle (LF), preovulatory estradiol (E2), and predominant vaginal epithelial cell at the completion of hormonal ovarian stimulation for fixed\u2010time artificial insemination (FTAI) in goats. Thirty\u2010seven crossbred Boer does received gonadotropin\u2010releasing hormone (GnRH) and intravaginal progesterone (P4)\u2010releasing devices (day 0). On day 5, P4 devices were removed and does received prostaglandin F<jats:sub>2\u03b1<\/jats:sub> and equine chorionic gonadotrophin. On day 7, does received GnRH, and FTAI was undertaken. On day 7, does were divided into three groups, i.e. small\u2010sized (3\u20133.9\u2009mm; <jats:italic>n<\/jats:italic>\u2009=\u20095), medium\u2010sized (4\u20134.9\u2009mm; <jats:italic>n<\/jats:italic>\u2009=\u20098), and large\u2010sized (\u22655\u2009mm; <jats:italic>n<\/jats:italic>\u2009=\u200924) according to the diameter of the ovarian LF; follicular characteristics (number and diameter) were identified, and blood samples and vaginal smears were collected. The average diameters of total antral follicles and LF and the percentage of superficial cell were greatest in large\u2010sized LF does (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.01). The average diameters of total antral follicle (<jats:italic>r<\/jats:italic>\u2009=\u2009.68) and LF (<jats:italic>r<\/jats:italic>\u2009=\u2009.71), number of preovulatory follicle (<jats:italic>r<\/jats:italic>\u2009=\u2009.58), and plasma E2 concentrations (<jats:italic>r<\/jats:italic>\u2009=\u2009.61) were positively correlated with the percentage of superficial cells (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.01). The likelihood of a pregnancy outcome after the FTAI increased by 13.71 times in does with a greater average diameter of antral follicle, 14.18 times with emergence of a large preovulatory follicle, and 36.83 times with a higher percentage of vaginal superficial cells (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.01). It was concluded that there is a relationship between the cell types of the vaginal epithelium, the diameters of the largest ovarian follicles, and the concentration of E2 in goats subjected to FTAI protocols.<\/jats:p>","DOI":"10.1111\/rda.14698","type":"journal-article","created":{"date-parts":[[2024,7,29]],"date-time":"2024-07-29T07:30:24Z","timestamp":1722238224000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Associations among the largest follicle, preovulatory estradiol concentrations, and predominant vaginal epithelial cells at the completion of hormonal ovarian stimulation for fixed\u2010time artificial insemination in goats"],"prefix":"10.1111","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-6128-7198","authenticated-orcid":false,"given":"Nalinthip","family":"Promsao","sequence":"first","affiliation":[{"name":"Department of Animal and Aquatic Sciences, Faculty of Agriculture Chiang Mai University  Chiang Mai Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7517-7834","authenticated-orcid":false,"given":"Punnawut","family":"Yama","sequence":"additional","affiliation":[{"name":"Department of Animal and Aquatic Sciences, Faculty of Agriculture Chiang Mai University  Chiang Mai Thailand"},{"name":"Science and Technology Park Chiang Mai University  Chiang Mai Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-8275-9222","authenticated-orcid":false,"given":"Assawadet","family":"Suriard","sequence":"additional","affiliation":[{"name":"Department of Animal and Aquatic Sciences, Faculty of Agriculture Chiang Mai University  Chiang Mai 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