{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T10:31:08Z","timestamp":1782988268454,"version":"3.54.5"},"reference-count":95,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,8,27]],"date-time":"2024-08-27T00:00:00Z","timestamp":1724716800000},"content-version":"vor","delay-in-days":26,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Heat stress (HS) disrupts testicular homeostasis because of oxidative stress. N\u2010acetylcysteine (NAC) is a thiol compound with antioxidants, anti\u2010inflammatory and anti\u2010apoptotic properties. As a sequel, this research aimed to assess the ameliorative effects of NAC supplementation on the reproductive performance of goat bucks kept under environmental HS. Primarily, Doppler examination as well as semen collection and evaluation were conducted on 12 mature bucks for 2\u2009weeks (W) as pre\u2010heat stress control (W1 and W2) during winter (February 2023). The temperature\u2013humidity index (THI) was 63.4\u201364.3 (winter season). Then during summer HS conditions (from the beginning of July till the end of August 2023) bucks were assessed before NAC supplementation (W0), afterwards they were arbitrarily assigned into two groups. The control group (CON; <jats:italic>n<\/jats:italic>\u2009=\u20096) received the basal diet while the NAC group (<jats:italic>n<\/jats:italic>\u2009=\u20096) received the basal diet in addition to oral NAC daily for 7\u2009weeks (W1\u2013W7). The THI was 78.1\u201381.6 (summer season). Testicular blood flow parameters, serum concentration of nitric oxide (NO) and testosterone were measured. Additionally, total antioxidant capacity (TAC) and malondialdehyde (MDA) content in seminal plasma and semen quality parameters were evaluated. There were marked reductions (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) in the resistive index (RI; W1, W4 and W5), pulsatility index (PI; W2 and W4\u2013W7), and systolic\/diastolic ratio (S\/D; W4\u2013W7) in the NAC group compared to the CON group. Furthermore, testosterone and NO levels were higher (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01 and <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05, respectively) in the NAC group (W2, W3, W5 and W3\u2013W5, respectively). Seminal plasma TAC increased (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) and MDA decreased (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) in the NAC group (W2, W4 and W5) compared to the CON group. Moreover, there were marked improvements (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) in semen quality parameters (mass motility, total motility, viability and normal morphology) in the NAC group. In conclusion, oral NAC supplementation could be used to enhance the reproductive performance of goat bucks during HS conditions which is supported by remarkable enhancement in testicular haemodynamics, NO, testosterone levels and semen quality parameters.<\/jats:p>","DOI":"10.1111\/rda.14709","type":"journal-article","created":{"date-parts":[[2024,8,27]],"date-time":"2024-08-27T10:19:54Z","timestamp":1724753994000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["N\u2010Acetylcysteine Supplementation Improves Testicular Haemodynamics, Testosterone Levels, Seminal Antioxidant Capacity and Semen Quality in Heat\u2010Stressed Goat Bucks"],"prefix":"10.1111","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9685-016X","authenticated-orcid":false,"given":"Ola","family":"Adel","sequence":"first","affiliation":[{"name":"Theriogenology Department, Faculty of Veterinary Medicine Cairo University  Giza Square Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0069-9926","authenticated-orcid":false,"given":"Hossam\u00a0R.","family":"El\u2010Sherbiny","sequence":"additional","affiliation":[{"name":"Theriogenology Department, Faculty of Veterinary Medicine Cairo University  Giza Square Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5519-0743","authenticated-orcid":false,"given":"Abdallah","family":"M.\u00a0Shahat","sequence":"additional","affiliation":[{"name":"Theriogenology Department, Faculty of Veterinary Medicine Cairo University  Giza Square Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7617-490X","authenticated-orcid":false,"given":"Sayed\u00a0Taha","family":"Ismail","sequence":"additional","affiliation":[{"name":"Theriogenology Department, Faculty of Veterinary Medicine Cairo University  Giza Square Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,8,27]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jat.1465"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.21608\/ejap.2022.147551.1043"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1515\/hmbci-2022-0050"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpedsurg.2019.09.028"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/1423-0127-17-S1-S35"},{"issue":"2","key":"e_1_2_9_7_1","first-page":"88","article-title":"Effects of N\u2010Acetyl Cysteine, Vitamin E and Vitamin C on Liver Glutathione Levels Following Amiodarone Treatment in Rats","volume":"16","author":"Akbay E.","year":"2019","journal-title":"Journal of Thoracic and Cardiovascular Surgery"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00383-009-2538-0"},{"key":"e_1_2_9_9_1","first-page":"1209","article-title":"Effect of Adding N\u2010Acetylcystiene and Avena Sativa Extract to Tris Extender on Post\u2010Cryopreservative Semen Characteristics of Holstein Bulls","volume":"20","author":"Ali M. 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