{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T16:07:24Z","timestamp":1785514044123,"version":"3.56.0"},"reference-count":32,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2024,9,10]],"date-time":"2024-09-10T00:00:00Z","timestamp":1725926400000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>This study aimed to evaluate the localised effects of intrauterine ozone therapy on endometrial recovery in mares with endometritis. Our investigation assessed changes in gene expression profiles of anti\u2010inflammatory (<jats:italic>IL\u20101RA<\/jats:italic> and <jats:italic>IL\u201010<\/jats:italic>), proinflammatory (<jats:italic>IL\u2010R1B3i<\/jats:italic> and <jats:italic>TNF\u03b1<\/jats:italic>) and pleiotropic (<jats:italic>IL\u20106<\/jats:italic>) cytokines, along with detailed histological measurements of epithelial and endometrial thickness and the glandular area ratio. Twenty mares were assigned to a 2 \u00d7\u20092 factorial design based on endometritis diagnosis and treatment (control or 42\u2009\u03bcg\/mL ozone insufflation), resulting in four groups: NC (negative for endometritis\/control), NO (negative\/ozone), PC (positive\/control) and PO (positive\/ozone). Oestrus was induced with 2\u2009mg of oestradiol benzoate on Days \u22121, 1 and 3, plus 1\u2009mg on Day 5. Day 0 marked the initial uterine treatment, followed by insufflations on Days 1 and 2 with O<jats:sub>3<\/jats:sub> (ozone) or O<jats:sub>2<\/jats:sub> (control). Uterine biopsies were taken before treatment on Day 0 and Day 6 for histological analysis and gene expression assessment. Data were analysed using a statistical model that included endometritis status, treatment type, biopsy times (D0 and D6) and their interactions, analysed with Proc Glimmix. Regardless of treatment or endometritis status, significant biopsy effects (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01) indicated increased epithelial height and endometrial thickness in Day 6 samples. Analysis of <jats:italic>IL\u20101<\/jats:italic> and <jats:italic>TNF\u03b1<\/jats:italic> revealed a significant interaction (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) among endometritis, treatment and biopsy, with higher <jats:italic>IL\u20101B3i<\/jats:italic> expression on Day 6 in the PC group. The treatment effect (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.04) showed a higher frequency (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01) of animals with positive modulation in the PC group (66.7%) versus the PO group (0.0%). An interaction effect (<jats:italic>p<\/jats:italic>\u2009=\u20090.08) between endometritis and treatment resulted from higher <jats:italic>IL\u20101RA<\/jats:italic> expression on Day 6 in the PC group compared to the PO group. Biopsy effect was significant for <jats:italic>IL\u201010<\/jats:italic> (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01), indicating higher values in the second sample associated with tissue repair. In the short\u2010term evaluation, ozone therapy did not influence endometrial morphology and may modulate cytokine expression, specifically the reduction in <jats:italic>IL\u20101<\/jats:italic> and <jats:italic>TNF\u03b1<\/jats:italic> levels. Therefore, this therapy appears to be a safe and potentially effective treatment for modulating the inflammatory response in mares with endometritis.<\/jats:p>","DOI":"10.1111\/rda.14718","type":"journal-article","created":{"date-parts":[[2024,9,10]],"date-time":"2024-09-10T09:15:47Z","timestamp":1725959747000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Does the Uterine Ozone Therapy Alter the Transcript Profile of Anti\u2010 and Proinflammatory Genes in Mares With Endometritis?"],"prefix":"10.1111","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7378-5644","authenticated-orcid":false,"given":"Jorge\u00a0Henrique\u00a0Villela","family":"Botelho","sequence":"first","affiliation":[{"name":"Universidade Professor Edson Ant\u00f4nio Velano  Alfenas Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7648-8810","authenticated-orcid":false,"given":"Gabriela\u00a0Oliveira","family":"Pessoa","sequence":"additional","affiliation":[{"name":"Federal University of Lavras\u2014UFLA  Lavras Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7780-8436","authenticated-orcid":false,"given":"Ester\u00a0Siqueira","family":"Caixeta","sequence":"additional","affiliation":[{"name":"Federal University of Alfenas\u2014UNIFAL  Alfenas Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-2912-4867","authenticated-orcid":false,"given":"Giuliana","family":"Sales","sequence":"additional","affiliation":[{"name":"Federal University of Lavras\u2014UFLA  Lavras Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-0457-7848","authenticated-orcid":false,"given":"Karine\u00a0Rabelo","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Federal University of Lavras\u2014UFLA  Lavras Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3185-0513","authenticated-orcid":false,"given":"Jos\u00e9\u00a0da\u00a0P\u00e1scoa","family":"Nascimento\u00a0Neto","sequence":"additional","affiliation":[{"name":"Federal University of Lavras\u2014UFLA  Lavras Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1629-4077","authenticated-orcid":false,"given":"Roger\u00a0Dinali","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Federal University of Lavras\u2014UFLA  Lavras Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6670-4946","authenticated-orcid":false,"given":"Miller\u00a0Pereira","family":"Palh\u00e3o","sequence":"additional","affiliation":[{"name":"Universidade Professor Edson Ant\u00f4nio Velano  Alfenas Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,9,10]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"63","volume-title":"Cellular and Molecular Immunology","author":"Abbas A. 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