{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T04:41:09Z","timestamp":1785386469836,"version":"3.55.0"},"reference-count":53,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2024,9,24]],"date-time":"2024-09-24T00:00:00Z","timestamp":1727136000000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100010719","name":"Pakistan Agricultural Research Council","doi-asserted-by":"publisher","award":["AS\u2010035"],"award-info":[{"award-number":["AS\u2010035"]}],"id":[{"id":"10.13039\/501100010719","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The objective of this study was to determine the optimal timing, sperm concentration, and body condition score (BCS) for laparoscopic artificial insemination (LAI) in the subtropical Lohi sheep breed. In Experiment 1, Lohi ewes (<jats:italic>n<\/jats:italic>\u2009= 80) were synchronised through progestin\u2010sponges (day 0\u2013day 11), administering PGF2<jats:sub>\u03b1<\/jats:sub> (<jats:sc>d<\/jats:sc>\u2010cloprostenol 75\u2009\u03bcg\/mL; i.m. @ 75\u2009\u03bcg \/ewe) on day 09 and eCG (i.m. @ 300\u2009IU\/ewe) on day 11. Ewes were divided equally into four groups (<jats:italic>n<\/jats:italic>\u2009=\u200920 each) and then LAI was performed at 48 (T48), 60 (T60), 72 (T72) and 96 (T96) hours post\u2010sponge removal using 200 million sperm\/insemination. In Experiment 2, ewes (<jats:italic>n<\/jats:italic>\u2009=\u200981) were synchronised as in Experiment I. Following synchronisation ewes were divided into four groups, each subjected to LAI employing varying sperm concentrations: 10 (S10; <jats:italic>n<\/jats:italic>\u2009=\u200921), 20 (S20; <jats:italic>n<\/jats:italic>\u2009=\u200920), 50 (S50; <jats:italic>n<\/jats:italic>\u2009=\u200920), or 100 (S100; <jats:italic>n<\/jats:italic>\u2009=\u200920) million per insemination. Inseminations were performed within a time window of 48\u201360\u2009h post\u2010sponge removal, based on the findings from Experiment 1. In both experiments ewes were categorised according to BCS, that is, medium 3\u2009and high &gt;\u20093. Results of Experiment 1 revealed a quadratic response that ewes inseminated at 60\u2010h post\u2010sponge removal exhibited significantly higher pregnancy and twinning rates (<jats:italic>p<\/jats:italic>\u2009=\u20090.02). The results of Experiment 2 revealed that sperm concentration had a quadratic effect, 50 million sperm per insemination resulted in maximal pregnancy rates (<jats:italic>p<\/jats:italic>\u2009=\u20090.01). Additionally, ewes with medium BCS (\u2264\u20093) had higher pregnancy rates than high BCS (&gt;\u20093) ewes. In conclusion, ewes with medium BCS (\u2264\u20093) are ideal candidate for LAI and can be inseminated at 60\u2009h with minimal sperm 50\u2009\u00d7\u200910<jats:sup>6<\/jats:sup>\/dose post\u2010sponge removal to achieve a maximum pregnancy rate.<\/jats:p>","DOI":"10.1111\/rda.14725","type":"journal-article","created":{"date-parts":[[2024,9,24]],"date-time":"2024-09-24T09:14:04Z","timestamp":1727169244000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Optimising Laparoscopic Artificial Insemination in Lohi Sheep: Effects of Timing, Sperm Concentration and Body Condition on Fertility Outcome in Subtropical Climates"],"prefix":"10.1111","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4624-1531","authenticated-orcid":false,"given":"Muhammad\u00a0Tayyab","family":"Khan","sequence":"first","affiliation":[{"name":"Department of Theriogenology, Faculty of Veterinary Science University of Veterinary and Animal Sciences  Lahore Pakistan"},{"name":"Department of Clinical Sciences, Faculty of Veterinary Science Bahauddin Zakariya University  Multan Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1992-7941","authenticated-orcid":false,"given":"Ejaz","family":"Ahmad","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, Faculty of Veterinary Science Bahauddin Zakariya University  Multan Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad\u00a0Rizwan","family":"Yousaf","sequence":"additional","affiliation":[{"name":"Department of Theriogenology, Faculty of Veterinary Science University of Veterinary and Animal Sciences  Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Oneeb","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Veterinary Science University of Veterinary and Animal Sciences  Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4532-483X","authenticated-orcid":false,"given":"Muhammad\u00a0Saleem","family":"Akhtar","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, Faculty of Veterinary Science Bahauddin Zakariya University  Multan Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2432-5753","authenticated-orcid":false,"given":"Muhammad\u00a0Irfan\u2010ur\u2010Rehman","family":"Khan","sequence":"additional","affiliation":[{"name":"Department of Theriogenology, Faculty of Veterinary Science University of Veterinary and Animal Sciences  Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,9,24]]},"reference":[{"issue":"1","key":"e_1_2_11_2_1","first-page":"37","article-title":"Body Condition Scoring of Local Ossimi Ewes at Mating and Its Impact 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