{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:45:22Z","timestamp":1753879522257,"version":"3.41.2"},"reference-count":37,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2024,9,30]],"date-time":"2024-09-30T00:00:00Z","timestamp":1727654400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100010712","name":"Viet Nam National University Ho Chi Minh City","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100010712","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Assisted reproductive technologies (ART) play a crucial role in conserving threatened wildlife species such as <jats:italic>Bos gaurus<\/jats:italic>. ART requires a large number of mature oocytes, and small antral follicles (SAFs) in the ovary are often used to obtain abundant sources of bovine oocytes. However, oocytes from SAFs often experience difficulty completing maturation and obtaining high quality and quantity of blastocyst formation compared to fully grown oocytes. This study aimed to increase the number of high\u2010quality mature oocytes and improve their potential for ART applications in cloned and interspecies intracytoplasmic sperm injection (ICSI) embryos by utilising L\u2010ascorbic acid (LAA) in pre in\u00a0vitro maturation (pre\u2010IVM) culture. First, oocytes isolated from SAFs were cultured with the duration of pre\u2010IVM 0, 6, 8, 10\u2009h and different concentrations of LAA to determine good conditions for oocyte maturation. Then, mature oocytes were assessed for their developmental competence through parthenogenesis, cloned and interspecies ICSI embryos. The results showed that 8\u2010h pre\u2010IVM with 50\u2009\u03bcg\/mL LAA improved the maturation rate and developmental competence of parthenogenetic and clone embryos, especially, improving the high blastocyst quality by increasing cell number and expression of histone acetylation at lysine 9 (H3K9ac). In addition, the culture process improved the nuclear reprogramming of somatic cells after nuclear transfer into mature oocytes, resulting in an increased hatching rate of cloned embryos. It also enhanced the activation and the pronuclear formation rate of Gaurus\u2010Taurus zygotes. Overall, the established pre\u2010IVM culture method enhanced the meiotic and developmental competence of embryos. This procedure opened hope for the preservation of endangered species and other applications.<\/jats:p>","DOI":"10.1111\/rda.14726","type":"journal-article","created":{"date-parts":[[2024,9,30]],"date-time":"2024-09-30T07:53:27Z","timestamp":1727682807000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Developmental Competence of Somatic Cell Nuclear Transfer Embryos and Interspecies <scp>ICSI<\/scp> Zygotes From Bovine Small Antral Follicles"],"prefix":"10.1111","volume":"59","author":[{"given":"Pham\u2010Truong","family":"Duy","sequence":"first","affiliation":[{"name":"Cellular Reprogramming Laboratory, School of Biotechnology International University  Ho Chi Minh City Vietnam"},{"name":"Vietnam National University  Ho Chi Minh City Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bui\u00a0Le\u00a0Quynh","family":"Nhu","sequence":"additional","affiliation":[{"name":"Cellular Reprogramming Laboratory, School of Biotechnology International University  Ho Chi Minh City Vietnam"},{"name":"Vietnam National University  Ho Chi Minh City Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pham\u00a0Quoc","family":"Dinh","sequence":"additional","affiliation":[{"name":"Cellular Reprogramming Laboratory, School of Biotechnology International University  Ho Chi Minh City Vietnam"},{"name":"Vietnam National University  Ho Chi Minh City Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cao\u00a0Hoang","family":"Nam","sequence":"additional","affiliation":[{"name":"Cellular Reprogramming Laboratory, School of Biotechnology International University  Ho Chi Minh City Vietnam"},{"name":"Vietnam National University  Ho Chi Minh City Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lam\u00a0Do\u00a0Truc","family":"Phuong","sequence":"additional","affiliation":[{"name":"Cellular Reprogramming Laboratory, School of Biotechnology International University  Ho Chi Minh City Vietnam"},{"name":"Vietnam National University  Ho Chi Minh City Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dao\u00a0Quang","family":"Tri","sequence":"additional","affiliation":[{"name":"Cellular Reprogramming Laboratory, School of Biotechnology International University  Ho Chi Minh City Vietnam"},{"name":"Vietnam National University  Ho Chi Minh City Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pham\u00a0Minh","family":"Chien","sequence":"additional","affiliation":[{"name":"Cellular Reprogramming Laboratory, School of Biotechnology International University  Ho Chi Minh City Vietnam"},{"name":"Vietnam National University  Ho Chi Minh City Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nhat\u2010Thinh","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Cellular Reprogramming Laboratory, School of Biotechnology International University  Ho Chi Minh City Vietnam"},{"name":"Vietnam National University  Ho Chi Minh City Vietnam"},{"name":"School of Medicine\u2010VNU  Ho Chi Minh City Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nguyen","family":"Van\u00a0Thuan","sequence":"additional","affiliation":[{"name":"Cellular Reprogramming Laboratory, School of Biotechnology International University  Ho Chi Minh City Vietnam"},{"name":"Vietnam National University  Ho Chi Minh City Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7170-3254","authenticated-orcid":false,"given":"Hong\u2010Thuy","family":"Bui","sequence":"additional","affiliation":[{"name":"Cellular Reprogramming Laboratory, School of Biotechnology International University  Ho Chi Minh City Vietnam"},{"name":"Vietnam National University  Ho Chi Minh City Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,9,30]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.20229"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.2018\u2010004"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP\u201017\u20100270"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD18109"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.20173"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP\u201006\u20100099"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.023747"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP\u201010\u20100109"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.103.026070"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.784244"},{"key":"e_1_2_9_12_1","unstructured":"Duckworth J. 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