{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:34:35Z","timestamp":1753878875342,"version":"3.41.2"},"reference-count":16,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2024,10,11]],"date-time":"2024-10-11T00:00:00Z","timestamp":1728604800000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>This study evaluated the effect of prostaglandin F2\u03b1 (PGF2\u03b1) associated with gonadotropin\u2010releasing hormone (GnRH) for ovulation induction in precocious indicus heifers submitted to a fixed\u2010time superovulation (SOV) programme. Precocious Nellore heifers (<jats:italic>n<\/jats:italic>\u2009=\u200935), aged 13\u2009months, were subjected to the SOV protocol. On day 0 (D0), all animals received intravaginal insertion of a progesterone (P4) device along with intramuscular administration of 2\u2009mg of oestradiol benzoate, plus 200\u2009IU of follicle\u2010stimulating hormone in decreasing doses, with 12\u2010h intervals between D4 and D7, in addition to 150\u2009\u03bcg of D\u2010cloprostenol on D6 and device removal on D7. On D8, the donors received 10.5\u2009\u03bcg of buserelin acetate and the treatment group received 300\u2009\u03bcg of D\u2010cloprostenol\/PGF<jats:sub>2\u03b1<\/jats:sub>. Artificial insemination was performed 12\u2009h and 24\u2009h after GnRH administration using frozen semen. On D15 of the protocol (i.e., D7 after insemination), the embryos were collected and evaluated. All animals passed through the control and treatment groups. Results were evaluated by analysis of variance using an adjusted mixed\u2010effects model (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). There was no difference in the total number of embryos between the control and treatment groups (10.40\u2009\u00b1\u20091.52 vs. 9.60\u2009\u00b1\u20091.36; <jats:italic>p<\/jats:italic>\u2009=\u20090.63) or viable embryos (6.30\u2009\u00b1\u20091.22 vs. 4.30\u2009\u00b1\u20090.71). For precocious indicus heifers, treatment with PGF<jats:sub>2\u03b1<\/jats:sub> in association with GnRH did not affect embryo production in the fixed\u2010time SOV protocol.<\/jats:p>","DOI":"10.1111\/rda.14734","type":"journal-article","created":{"date-parts":[[2024,10,11]],"date-time":"2024-10-11T11:05:29Z","timestamp":1728644729000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effect of the Application of <scp>PGF<sub>2\u03b1<\/sub><\/scp> Associated With Ovulation Induction in a Fixed\u2010Time Superovulation Programme for Precocious Nellore Heifers"],"prefix":"10.1111","volume":"59","author":[{"given":"Tamires\u00a0Korchovei","family":"Sanches","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Reprodu\u00e7\u00e3o Animal DCV\u2010CCA\u2010UEL  Londrina Parana Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pamela\u00a0Mara\u00a0Celestino","family":"Soares","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Reprodu\u00e7\u00e3o Animal DCV\u2010CCA\u2010UEL  Londrina Parana Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcio\u00a0de\u00a0Oliveira","family":"Marques","sequence":"additional","affiliation":[{"name":"Geraembryo  Corn\u00e9lio Proc\u00f3pio Parana Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rubens\u00a0Cesar\u00a0Pinto","family":"da Silva","sequence":"additional","affiliation":[{"name":"Geraembryo  Corn\u00e9lio Proc\u00f3pio Parana Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luiz\u00a0Francisco\u00a0Machado","family":"Pfeifer","sequence":"additional","affiliation":[{"name":"Embrapa, Brazilian Agricultural Research Corporation  Porto Velho Rond\u00f4nia Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amanda\u00a0Fonseca","family":"Zangirolamo","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Reprodu\u00e7\u00e3o Animal DCV\u2010CCA\u2010UEL  Londrina Parana Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4476-6449","authenticated-orcid":false,"given":"F\u00e1bio","family":"Morotti","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Reprodu\u00e7\u00e3o Animal DCV\u2010CCA\u2010UEL  Londrina Parana Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5097-5119","authenticated-orcid":false,"given":"Marcelo\u00a0Marcondes","family":"Seneda","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Reprodu\u00e7\u00e3o Animal DCV\u2010CCA\u2010UEL  Londrina Parana Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,10,11]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0620283"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2012-5368"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.21451\/1984-3143-AR2019-0050"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.21451\/1984-3143-AR2018-0091"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2019.00467"},{"key":"e_1_2_9_7_1","first-page":"81","volume-title":"Reproduction in Mammals. Book 7. Mechanisms of Hormone Action","author":"Challis J. R. G.","year":"1979"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2021-20281"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1590\/0103-8478cr20210629"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2012.06.030"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2020.04.038"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2022-22651"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2013.11.016"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-204X2016000600005"},{"key":"e_1_2_9_15_1","unstructured":"Rizzo A. P.D'Amico R.Minoia G.Aiudi andR. L.Sciorsci.2003. \u201cSomministrazione di PGF2a al Momento Della F. A. in Bovine Repeat Breeder. Atti Convegno Della Societa' Italiana di Riproduzione Animale (SIRA).\u201d 6\u20139."},{"key":"e_1_2_9_16_1","first-page":"112","volume-title":"Manual of the International Embryo Transfer Society","author":"Stringfellow D. 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