{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T18:23:33Z","timestamp":1785522213639,"version":"3.56.0"},"reference-count":25,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2024,11,4]],"date-time":"2024-11-04T00:00:00Z","timestamp":1730678400000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>As the hybrid between cattle and yak, cattleyak is a typical male sterile mammal, and the underlying mechanism for its spermatogenic arrest is still unclear. In this study, the coding region of cattleyak <jats:italic>TAF4B<\/jats:italic> gene was cloned by RT\u2010PCR and analysed by bioinformatics. To investigate the effects of <jats:italic>TAF4B<\/jats:italic> on cellular proliferation and differentiation, an expression vector was generated and introduced into undifferentiated spermatogonia (UDSPG) of cattleyak. The results showed that the protein encoded by <jats:italic>TAF4B<\/jats:italic> did not contain the signal peptide sequence. The expression level of <jats:italic>TAF4B<\/jats:italic> in UDSPG of cattleyak was lower than that in yak, while the overexpression of <jats:italic>TAF4B<\/jats:italic> in cattleyak promoted the proliferation activity of cattleyak UDSPG. Meanwhile, the expression of proliferation and meiosis\u2010related genes was increased but the differentiation\u2010related genes were decreased. Therefore, the aberrant expression of <jats:italic>TAF4B<\/jats:italic> in cattleyak UDSPG possibly impaired its proliferation and differentiation equilibrium and decreased its growth potentiality, thereby reducing the quantity of UDSPG and affecting spermatogenesis. This study provided a potential approach for further elucidation of the mechanism of spermatogenesis arrest and provided a new idea for solving the problem of male infertility in cattleyak.<\/jats:p>","DOI":"10.1111\/rda.14738","type":"journal-article","created":{"date-parts":[[2024,11,4]],"date-time":"2024-11-04T11:55:22Z","timestamp":1730721322000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Overexpression of <i>TAF4B<\/i> Promoted the Proliferation of Undifferentiated Spermatogonia in Cattleyak In\u00a0Vitro"],"prefix":"10.1111","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-6650-2456","authenticated-orcid":false,"given":"Yuan","family":"Tian","sequence":"first","affiliation":[{"name":"Key Laboratory of Qinghai\u2010Tibetan Plateau Animal Genetic Resource Reservation and Utilization of Sichuan Province and Ministry of Education Southwest Minzu University  Chengdu Sichuan 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