{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T02:23:45Z","timestamp":1793327025972,"version":"4.5.0"},"reference-count":28,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2024,11,11]],"date-time":"2024-11-11T00:00:00Z","timestamp":1731283200000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The success of artificial insemination (AI) with frozen\u2013thawed semen in cattle is influenced by both female factors and sperm quality. In terms of sperm quality, prior studies indicate that the ability of frozen\u2013thawed bovine sperm to fertilise an oocyte is dependent on their quality and resilience to cryopreservation. Cryopreservation induces oxidative stress, leading to ultrastructural damage in the sperm. This study aimed to determine whether the quality of fresh semen can identify bulls with good and poor sperm freezability. This difference between fresh and frozen semen from the same bull allows us to predict fertility. Motility and kinetic parameters were assessed using computer\u2010assisted sperm analysis (CASA), while six functional variables were evaluated through flow cytometry, both before and after the freeze\u2013thaw process on the sperm from 13 bulls. In\u00a0vivo fertility was measured using 90\u2010day non\u2010return rates. The principal component analysis (PCA) of eight sperm variables post\u2010thaw identified one principal component explaining 81.19% of the total variance and classified the bulls into two groups: Poor freezability bulls (progressive motility: 48.12%\u2009\u00b1\u20098.41%; viability: 77.51%\u2009\u00b1\u20097.61%) and good freezability bulls (progressive motility: 58.64%\u2009\u00b1\u20096.64%; viability: 88.12%\u2009\u00b1\u20092.52%). Bulls with higher freezability showed better sperm viability and motility, as well as lower levels of ROS, superoxides and intracellular calcium before cryopreservation that were significantly correlated with higher non\u2010return rates (NRR). The results underscore the importance of assessing the quality and functionality of fresh semen to predict the fertility potential of cryopreserved sperm. This approach can aid in selecting ejaculates with the best potential for successful artificial insemination, ultimately improving reproductive performance in dairy cattle.<\/jats:p>","DOI":"10.1111\/rda.14742","type":"journal-article","created":{"date-parts":[[2024,11,12]],"date-time":"2024-11-12T02:33:57Z","timestamp":1731378837000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Predictive Indicators of Cryotolerance and Fertility in Bovine Sperm: Evaluating Fresh Semen Quality to Improve\n                    <scp>AI<\/scp>\n                    Outcomes With Frozen\u2013Thawed Sperm"],"prefix":"10.1111","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4484-8981","authenticated-orcid":false,"given":"Carolina","family":"Tamargo","sequence":"first","affiliation":[{"name":"Selecci\u00f3n y Reproducci\u00f3n Animal\u2014SERIDA Principado de Asturias  Gij\u00f3n Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9445-4438","authenticated-orcid":false,"given":"Ferran","family":"Garriga","sequence":"additional","affiliation":[{"name":"Biotechnology of Animal and Human Reproduction (TechnoSperm), Institute of Food and Agricultural Technology University of Girona  Girona Spain"},{"name":"Unit of Cell Biology, Department of Biology, Faculty of Sciences University of Girona  Girona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2209-340X","authenticated-orcid":false,"given":"Marc","family":"Yeste","sequence":"additional","affiliation":[{"name":"Biotechnology of Animal and Human Reproduction (TechnoSperm), Institute of Food and Agricultural Technology University of Girona  Girona Spain"},{"name":"Unit of Cell Biology, Department of Biology, Faculty of Sciences University of Girona  Girona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5488-4417","authenticated-orcid":false,"given":"Elisabeth","family":"Pinart","sequence":"additional","affiliation":[{"name":"Biotechnology of Animal and Human Reproduction (TechnoSperm), Institute of Food and Agricultural Technology University of Girona  Girona Spain"},{"name":"Unit of Cell Biology, Department of Biology, Faculty of Sciences University of Girona  Girona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9518-808X","authenticated-orcid":false,"given":"Rodrigo","family":"Mui\u00f1o","sequence":"additional","affiliation":[{"name":"Department of Animal Pathology, Faculty of Veterinary Medicine\/IBADER, Campus Terra University of Santiago de Compostela  Lugo Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0278-4537","authenticated-orcid":false,"given":"Mar\u00eda Teresa","family":"Carbajo","sequence":"additional","affiliation":[{"name":"Department of Medicine, Surgery and Veterinary Anatomy Universidad de Le\u00f3n  Le\u00f3n Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9810-1517","authenticated-orcid":false,"given":"Carlos Olegario","family":"Hidalgo","sequence":"additional","affiliation":[{"name":"Selecci\u00f3n y Reproducci\u00f3n Animal\u2014SERIDA Principado de Asturias  Gij\u00f3n 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