{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T12:01:19Z","timestamp":1764331279120,"version":"3.41.2"},"reference-count":34,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2024,11,25]],"date-time":"2024-11-25T00:00:00Z","timestamp":1732492800000},"content-version":"vor","delay-in-days":24,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Antioxidants help safeguard sperm cells from damage during the freeze\u2013thaw process. Melatonin and its metabolites have an antioxidative effect. The current study aimed to evaluate the impact of melatonin supplementation in tris\u2010based extenders at various concentrations (0.5, 1.0 and 2\u2009mM) on the freezability of Jamnapari goat spermatozoa. A total of 48 ejaculates were collected twice a week from four Jamnapari bucks (<jats:italic>n<\/jats:italic>\u2009=\u200912\u2009\u00d7\u20094) using an artificial vagina during the period of October to November 2023. Selected ejaculates diluted with tris\u2010citric acid egg yolk extender were divided into four equal aliquots, and melatonin (dissolved in 0.1% dimethyl sulfoxide) was added later at 0.5, 1.0 and 2.0\u2009mM to the control group (C) (extender and 0.1% DMSO) and treatment groups T1, T2 and T3, respectively. Various seminal attributes such as progressive motility, livability, acrosomal integrity, sperm abnormalities, sperm plasma membrane integrity, sperm penetration distance by vanguard spermatozoa in polyacrylamide gel and seminal plasma enzyme leakage (AST, ALT, ACP and AKP) were evaluated at post\u2010dilution and post\u2010thawed stages. Our findings revealed that all semen quality parameters were superior in melatonin\u2010treated groups than the C, and the differences were noticeably higher in the T2 group (1.0\u2009mM) than the other groups. Adding 1.0\u2009mM melatonin proved to be the most effective to safeguard sperm cells from cryopreservation induced cryodamage of Jamnapari buck.<\/jats:p>","DOI":"10.1111\/rda.14743","type":"journal-article","created":{"date-parts":[[2024,11,25]],"date-time":"2024-11-25T09:44:52Z","timestamp":1732527892000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The Effect of Different Concentrations of Melatonin on the Quality of Frozen and Thawed Semen of Jamnapari Goat"],"prefix":"10.1111","volume":"59","author":[{"given":"Kabir","family":"Alam","sequence":"first","affiliation":[{"name":"Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science and Animal Husbandry Acharya Narendra Deva University of Agriculture and Technology  Ayodhya India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sushant","family":"Srivastava","sequence":"additional","affiliation":[{"name":"Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science and Animal Husbandry Acharya Narendra Deva University of Agriculture and Technology  Ayodhya India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sanjeev Kumar","family":"Verma","sequence":"additional","affiliation":[{"name":"Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science and Animal Husbandry Acharya Narendra Deva University of Agriculture and Technology  Ayodhya India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abhishek Kumar","family":"Verma","sequence":"additional","affiliation":[{"name":"Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science and Animal Husbandry Acharya Narendra Deva University of Agriculture and Technology  Ayodhya India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Saurabh","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Complex, College of Veterinary Science and Animal Husbandry Acharya Narendra Deva University of Agriculture and Technology  Ayodhya India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rajesh","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science and Animal Husbandry Acharya Narendra Deva University of Agriculture and Technology  Ayodhya India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rabindra","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science and Animal Husbandry Acharya Narendra Deva University of Agriculture and Technology  Ayodhya India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ankit","family":"Jaiswal","sequence":"additional","affiliation":[{"name":"Department of Livestock Production Management, College of Veterinary Science and Animal Husbandry Acharya Narendra Deva University of Agriculture and Technology  Ayodhya India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Azeer","family":"Alam","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, College of Veterinary Science and Animal Husbandry Acharya Narendra Deva University of Agriculture and Technology  Ayodhya India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7680-6444","authenticated-orcid":false,"given":"Tanmoy","family":"Rana","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Complex West Bengal University of Animal &amp; Fishery Sciences  Kolkata India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,11,25]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.4102\/jsava.v85i1.972"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.2000.tb03268.x"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.4103\/2305-0500.268142"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2795(200003)55:3<282::AID-MRD6>3.0.CO;2-7"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1052"},{"key":"e_1_2_9_7_1","first-page":"238","article-title":"Effects of Melatonin on Cryopreserved Semen Parameters and Apoptosis of Thai Swamp Buffalo Bull (\n                     Bubalus bubalis\n                  ) in Different Thawing Conditions","volume":"9","author":"Chaudhary S. 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