{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T03:42:54Z","timestamp":1784691774224,"version":"3.55.0"},"reference-count":35,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,1,29]],"date-time":"2025-01-29T00:00:00Z","timestamp":1738108800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Currently, incubators with a time\u2010lapse system are widely used for in\u00a0vitro embryo production in several species, however, their effect on alpaca embryo development compared to conventional incubators remains unknown. The aim of this study was to compare early in\u00a0vitro embryo development in alpacas using a time\u2010lapse incubator system versus a conventional incubator. Ovaries were obtained from a slaughterhouse and 1048 cumulus\u2010oocyte complexes (COCs) were collected and in\u00a0vitro matured for 26\u2009h in either a time\u2010lapse system (<jats:italic>n<\/jats:italic>\u2009=\u2009542) or a conventional incubator (<jats:italic>n<\/jats:italic>\u2009=\u2009542). All the matured COCs were fertilised for 24\u2009h with fresh spermatozoa recovered from epididymis obtained from the slaughterhouse. The presumptive zygotes in both systems were cultured in a commercial one\u2010step culture medium, and the embryo development was evaluated at 48, 120 and 168\u2009h after fertilisation. Our results showed no differences in maturation rates between the time\u2010lapse system and the conventional incubator (79.9% vs. 55.0%, <jats:italic>p<\/jats:italic>\u2009=\u20090.080). Cleavage rates at 48\u2009h were also similar between the time\u2010lapse and conventional systems: 20.5% vs. 20.8% for embryos at the two\u2010 to four\u2010cell stage, and 6.0% vs. 7.0% for embryos above the four\u2010cell stage (<jats:italic>p<\/jats:italic>\u2009&gt;\u20090.05). At 120\u2009h after fertilisation, the percentage of embryos were more evenly distributed across all stages (below eight\u2010cell, eight\u2010cell and above eight\u2010cell stages) in both systems (<jats:italic>p<\/jats:italic>\u2009&gt;\u20090.05). At 168\u2009h, no differences in the blastocyst rates and blastocyst quality were observed between the time\u2010lapse and conventional systems. In conclusion, in\u00a0vitro embryo production in alpacas can be successfully carried out using the time\u2010lapse incubation system or a conventional incubator with comparable results. Further study would focus on the correlation between the developmental patterns and the quality of alpaca embryos, as well as the comparison of in\u00a0vivo embryo development between the two incubation systems.<\/jats:p>","DOI":"10.1111\/rda.70008","type":"journal-article","created":{"date-parts":[[2025,1,29]],"date-time":"2025-01-29T13:29:44Z","timestamp":1738157384000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Effect of Time\u2010Lapse Incubation System on In Vitro Development of Alpaca Embryos"],"prefix":"10.1111","volume":"60","author":[{"given":"Cendy Lutz Ccasa","family":"Ccahuana","sequence":"first","affiliation":[{"name":"Veterinary Embryology Laboratory, Professional School of Veterinary Medicine Universidad Nacional de San Antonio Abad del Cusco  Sicuani\u2010Cusco Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro Walter Bravo","family":"Matheus","sequence":"additional","affiliation":[{"name":"Animal Reproduction Laboratory, Professional School of Veterinary Medicine Universidad Nacional de San Antonio Abad del Cusco  Sicuani\u2010Cusco Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5680-5110","authenticated-orcid":false,"given":"Ying","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Animal, Dairy and Veterinary Sciences Utah State University  Logan Utah USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guiulfo Duriel","family":"Mamani\u2010Mango","sequence":"additional","affiliation":[{"name":"Veterinary Embryology Laboratory, Professional School of Veterinary Medicine Universidad Nacional de San Antonio Abad del Cusco  Sicuani\u2010Cusco Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,1,29]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3923\/ajava.2016.737.756"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.14269"},{"key":"e_1_2_10_4_1","first-page":"344","article-title":"Evaluation and Classification of Bovine Embryos","volume":"10","author":"B\u00f3 G.","year":"2018","journal-title":"Animal Reproduction"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0015\u20100282(98)00395\u20101"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smallrumres.2022.106680"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.5653\/cerm.2020.04091"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gine.2021.100709"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2017.12.025"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2020.10.03"},{"key":"e_1_2_10_11_1","unstructured":"Gutierrez G. 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