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An ultrasound ovarian examination was carried out in 406 crossbred suckled cows at progesterone (P4) insert removal on D8 of a TAI protocol and at pregnancy diagnosis 30\u2009days after TAI. Follicular (antrum, wall and perifollicular stroma) and luteal morphometry, and echotexture and heterogeneity parameters were analysed on D8 and D30 after TAI, respectively. Follicles that did not reach divergence (&lt;\u20098.5\u2009mm) at P4 removal had higher antral echotexture and heterogeneity values (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0001) than those that surpassed divergence (&gt;\u20098.5\u2009mm). Lower follicular antrum echotexture levels at P4 removal positively correlated with subsequent estrus expression and pregnancy outcomes (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05), and negatively with follicle size (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0001). Luteal echotexture varied according to the originating follicle size (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05), with no differences between pregnant and non\u2010pregnant cows 30\u2009days after TAI. Cows had higher odds of estrus expression (Se, 70.61%; Sp, 61.05%; AUC 0.80; <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0001) and attaining P\/AI (Se, 64.52%; Sp, 61.05%; AUC 0.73; <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0001) with lower follicular antrum echotexture (overall cut\u2010off \u2264\u200914.25). The results show that echotexture values in antral follicles on D8 are strongly associated with pregnancy outcomes and may be useful for predicting success in TAI protocols.<\/jats:p>","DOI":"10.1111\/rda.70018","type":"journal-article","created":{"date-parts":[[2025,3,9]],"date-time":"2025-03-09T09:05:58Z","timestamp":1741511158000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Follicular B\u2010Mode Echotexture Parameters at <scp>P<\/scp>4 Insert Removal and Their Association With Pregnancy Outcomes in Beef Cows Undergoing Timed <scp>AI<\/scp> Protocol"],"prefix":"10.1111","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6704-5137","authenticated-orcid":false,"given":"C\u00e9sar Augusto","family":"Pinz\u00f3n\u2010Osorio","sequence":"first","affiliation":[{"name":"Embryology and Reproductive Technology Lab, School of Veterinary Medicine Universidade Federal do Rio Grande do Sul  Porto Alegre 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