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Successful spermatogenesis is inseparable from niche regulation, which provides factors that enhance the self\u2010renewal of SSCs to maintain their numbers and directs the appropriate differentiation of spermatogonia. Some progress has been achieved in the definition and isolation of SSCs. However, a high degree of cellular heterogeneity is found in the testis, revealing a combination of various cell types at different developmental stages and a lack of specific molecular markers (especially in domestic animals) for fully screening and purifying SSCs. These factors have considerably hindered further research into the mechanisms of maintenance, self\u2010renewal, and differentiation of SSCs, as well as limited their isolation, purification, and applications. Accumulated studies have recently successfully employed single\u2010cell RNA sequencing (scRNA\u2010seq) as a novel approach to detailing the classification of cell subsets, mining specifically expressed genes in different cell types, and accurate identification of specific cell types. 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