{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T08:37:21Z","timestamp":1777624641904,"version":"3.51.4"},"reference-count":63,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,3,3]],"date-time":"2025-03-03T00:00:00Z","timestamp":1740960000000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>This study evaluated the effect of equine chorionic gonadotropin (eCG) on ovarian vascularisation and plasma progesterone (P4) levels in Murrah buffaloes during an ovulation synchronisation protocol. Twenty buffaloes were divided into two groups: with eCG (<jats:italic>n<\/jats:italic>\u2009=\u200920) and without eCG (control, <jats:italic>n<\/jats:italic>\u2009=\u200920) in a crossover design. A 1.0\u2009g progesterone intravaginal device (DIB) was inserted and 2\u2009mg oestradiol benzoate was administered intramuscularly on Day 0. On Day 9, DIB was removed, PGF2\u03b1 was administered to all animals and eCG was given to half. GnRH was administered on Day 11. Daily Doppler ultrasounds assessed follicular and luteal development and vascularisation from D9 to D16 and on Days 20, 24, 28 and 32. Blood samples were collected before each ultrasound to analyse plasma P4. Ovulation occurred on Day 13.42\u2009\u00b1\u20091.17 in the eCG group (19\/20) and 13.53\u2009\u00b1\u20090.19 (14\/20) in the control (<jats:italic>p<\/jats:italic>\u2009=\u20090.34). The ovulation rate was higher in the eCG group (95%) than in the control (70%). eCG increased the vascularised follicle perimeter on Days 11 (<jats:italic>p<\/jats:italic>\u2009=\u20090.018) and 12 (<jats:italic>p<\/jats:italic>\u2009=\u20090.03) and enhanced corpus luteum (CL) diameter on Day 16 (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001). A larger vascularised area was observed on Days 14\u201316, 20 and 24 (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). P4 concentrations were higher in the eCG group on Days 15, 16, 20 and 24 (p\u2009&lt;\u20090.05). Significant correlations were found between CL size, vascularisation and P4 concentration (r\u2009=\u20090.75, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001). In conclusion, eCG improves ovarian vascularisation, ovulation rates and plasma P4 levels, supporting its use to enhance reproductive performance in buffalo herds.<\/jats:p>","DOI":"10.1111\/rda.70027","type":"journal-article","created":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T16:50:02Z","timestamp":1741711802000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Effect of Equine Chorionic Gonadotropin on Follicular and Luteal Vascularisation and Progesterone Production in Murrah Buffaloes Subjected to Ovulation Synchronisation"],"prefix":"10.1111","volume":"60","author":[{"given":"Felipe Rydygier","family":"de Ruediger","sequence":"first","affiliation":[{"name":"Agricultural Sciences, Veterinary Medicine course, Department of Animal Reproduction Western S\u00e3o Paulo University, Presidente Prudente  S\u00e3o Paulo SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N\u00e9lcio Antonio T.","family":"de Carvalho","sequence":"additional","affiliation":[{"name":"Registro Regional Research Center\/Institute of Animal Science, Registro, S\u00e3o Paulo\u2010SP; Agricultural Defense Office  Registro SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Viviane M.","family":"Codognoto","sequence":"additional","affiliation":[{"name":"Department of Veterinary Surgery and Animal Reproduction S\u00e3o Paulo State University (Unesp), School of Veterinary Medicine and Animal Science  Botucatu Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ariane","family":"Dantas","sequence":"additional","affiliation":[{"name":"Department of Veterinary Surgery and Animal Reproduction S\u00e3o Paulo State University (Unesp), School of Veterinary Medicine and Animal Science  Botucatu Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paulo H.","family":"Yamada","sequence":"additional","affiliation":[{"name":"Department of Veterinary Surgery and Animal Reproduction S\u00e3o Paulo State University (Unesp), School of Veterinary Medicine and Animal Science  Botucatu Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rog\u00e9rio Ant\u00f4nio","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Department of Veterinary Surgery and Animal Reproduction S\u00e3o Paulo State University (Unesp), School of Veterinary Medicine and Animal Science  Botucatu Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo\u00e3o Carlos","family":"Pinheiro","sequence":"additional","affiliation":[{"name":"Department of Veterinary Surgery and Animal Reproduction S\u00e3o Paulo State University (Unesp), School of Veterinary Medicine and Animal Science  Botucatu Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9694-0456","authenticated-orcid":false,"given":"Rog\u00e9rio A.","family":"Almeida Filho","sequence":"additional","affiliation":[{"name":"Department of Veterinary Surgery and Animal Reproduction S\u00e3o Paulo State University (Unesp), School of Veterinary Medicine and Animal Science  Botucatu Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eunice","family":"Oba","sequence":"additional","affiliation":[{"name":"Department of Veterinary Surgery and Animal Reproduction S\u00e3o Paulo State University (Unesp), School of Veterinary Medicine and Animal Science  Botucatu Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,3,3]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod38.4.756"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1530\/rep.0.1250759"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2004.04.022"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2007.03.011"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0967199411000578"},{"key":"e_1_2_10_7_1","first-page":"174","article-title":"Angiog\u00eanese, vasculariza\u00e7\u00e3o e uso do ultrassom Doppler colorido na avalia\u00e7\u00e3o de estruturas ovarianas","volume":"36","author":"Ayres H.","year":"2012","journal-title":"Revista Brasileira de Reprodu\u00e7\u00e3o Animal"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.livprodsci.2004.06.014"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.4081\/ijas.2007.s2.663"},{"key":"e_1_2_10_10_1","first-page":"234","volume-title":"Calving Distribution Throughout the Year in Buffalo Raised All Over Brazil. 6th World Buffalo Congress","author":"Baruselli P. S.","year":"2001"},{"key":"e_1_2_10_11_1","first-page":"119","article-title":"Reproduction Management and Artificial Insemination in Buffalo","volume":"1","author":"Baruselli P. S.","year":"2002","journal-title":"1 Buffalo Symposium of Americas"},{"key":"e_1_2_10_12_1","first-page":"107","article-title":"Fixed\u2010Time Artificial Insemination in Buffalo","volume":"6","author":"Baruselli P. S.","year":"2007","journal-title":"Italian Journal of Animal Science"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2020.01.037"},{"issue":"1","key":"e_1_2_10_14_1","first-page":"160","article-title":"Control of Buffalo Follicular Dynamics for Artificial Insemination, Superovulation and In\u00a0Vitro Embryo Production","volume":"32","author":"Baruselli P. S.","year":"2013","journal-title":"Buffalo Bulletin"},{"issue":"3","key":"e_1_2_10_15_1","first-page":"293","article-title":"Bubalinocultura no Brasil: situa\u00e7\u00e3o e import\u00e2ncia econ\u00f4mica","volume":"31","author":"Bernardes O.","year":"2007","journal-title":"Revista Brasileira de Reprodu\u00e7\u00e3o Animal"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093\u2010691X(01)00646\u2010X"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod30.3.585"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2016.04.033"},{"key":"e_1_2_10_19_1","first-page":"1","article-title":"Desestacionaliza\u00e7\u00e3o dos partos para a produ\u00e7\u00e3o de leite de B\u00fafalas a pasto no Centro Sul do Brasil","volume":"8","author":"Carvalho N. A. T.","year":"2011","journal-title":"Pesquisa & Tecnologia"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2012.10.013"},{"key":"e_1_2_10_21_1","first-page":"527","article-title":"Use of Different Progestagens for Ovulation Synchronization and TAI in Buffaloes During the Non Breeding Season","volume":"32","author":"Carvalho N. A. T.","year":"2013","journal-title":"Buffalo Bulletin"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2013.11.004"},{"key":"e_1_2_10_23_1","first-page":"47","article-title":"New Strategies to Improve the Efficiency of the Ovsynch Protocol in Primiparous Dairy Cows","volume":"51","author":"Cirit \u00dc.","year":"2007","journal-title":"Bulletin of the Veterinary Institute in Pulawy"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani14182642"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2013.01.003"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD15223"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0303\u20107207(02)00056\u20104"},{"key":"e_1_2_10_28_1","first-page":"159","article-title":"Oestrus Detection in Buffaloes (Bubalus bubalis): Use of an Androgenized Female","volume":"1","author":"Drost M.","year":"1985","journal-title":"Buffalo Bulletin"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2019.11.004"},{"key":"e_1_2_10_30_1","volume-title":"Ultrasonic Imaging and Animal Reproduction: Color\u2010Doppler Ultrasonography","author":"Ginther O. J.","year":"2007"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2007\u20100044"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2009.11.016"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0630491"},{"key":"e_1_2_10_34_1","volume-title":"Estat\u00edstica: teoria e aplica\u00e7\u00f5es","author":"Levine D. M.","year":"2000"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.20305"},{"issue":"1","key":"e_1_2_10_36_1","first-page":"21","article-title":"Impact of PGF2\u0391 Analogue with or Without GnRH on the Subsequent Conception Rate in Anestrus Nili Ravi Buffalo","volume":"1","author":"Majid H. A.","year":"2022","journal-title":"Journal of Biological Sciences"},{"key":"e_1_2_10_37_1","first-page":"392","volume-title":"Efeitos da administra\u00e7\u00e3o de eCG e de Benzoato de Estradiol para sincroniza\u00e7\u00e3o da ovula\u00e7\u00e3o em vacas zebu\u00ednas no per\u00edodo p\u00f3s\u2010parto","author":"Marques M. O.","year":"2003"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2008.02.027"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod65.4.1014"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2014.07.026"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.5713\/ajas.2009.80524"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/asj.12400"},{"key":"e_1_2_10_43_1","first-page":"344","article-title":"Reproductive Performance of Dairy Buffaloes Submitted to TAI Protocols Based on P4\/E2 Plus eCG During Breeding and Nonbreeding Seasons","volume":"11","author":"Monteiro B. M.","year":"2014","journal-title":"Animal Reproduction"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod35.5.1187"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1210\/edrv\u201012\u20101\u201027"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2008.12.012"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2008.01.008"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11250-020-02381-9"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.11606\/T.10.2004.tde-18072005-082046"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2004.07.024"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1385\/ENDO:12:1:1"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP\u201009\u20100283"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10930\u2010023\u201010092\u2010x"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2014.03.005"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2009.11.004"},{"key":"e_1_2_10_56_1","volume-title":"Statistical Analysis System 2009","author":"SAS Institute","year":"2009"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439\u20100531.2004.00509.x"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD09251"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2016.04.031"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2008.12.025"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.21451\/1984\u20103143\u2010AR797"},{"key":"e_1_2_10_62_1","volume-title":"Bubalinos: fisiologia e patologia da reprodu\u00e7\u00e3o","author":"Vale W. G.","year":"1988"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093\u2010691X(01)00565\u20109"},{"key":"e_1_2_10_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2007.06.007"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.70027","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,8,26]],"date-time":"2025-08-26T05:48:13Z","timestamp":1756187293000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.70027"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3]]},"references-count":63,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,3]]}},"alternative-id":["10.1111\/rda.70027"],"URL":"https:\/\/doi.org\/10.1111\/rda.70027","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3]]},"assertion":[{"value":"2024-11-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70027"}}