{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,27]],"date-time":"2025-08-27T00:05:41Z","timestamp":1756253141130,"version":"3.44.0"},"reference-count":34,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,3,7]],"date-time":"2025-03-07T00:00:00Z","timestamp":1741305600000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The advancement of short\u2010term storage methods for collared peccary semen targets its potential application in artificial insemination programmes and for combination with cryopreservation techniques. The objective of this study was to evaluate the performance of a transport container (Botutainer) for the preservation of collared peccary semen using commercial extenders (BTS, NUTRIXcell+ and PRIMXcell Ultra) as well as a TRIS + egg yolk extender. Ten ejaculates obtained by electroejaculation were diluted and stored at 5\u00b0C for 72\u2009h. The cooled samples were analysed for kinetic aspects (using a computerised system), membrane functionality (hyposmotic test), membrane integrity and mitochondrial activity (fluorescent probes), morphology (Rose Bengal staining) and sperm binding capacity (to the perivitelline membrane of the egg yolk). After 72\u2009h of storage, TRIS, NUTRIXcell and PRIMXcell preserved approximately 60% of motile sperm and 50%\u201365% of membrane integrity. In contrast, BTS was not effective in maintaining these parameters, preserving only ~40% and 50%, respectively (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). All extenders preserved ~50% to 65% of mitochondrial activity and 72%\u201378% of normal sperm morphology up to 72\u2009h. TRIS was the only extender that preserved ~75% of membrane functionality for 72\u2009h. Finally, BTS exhibited a reduction in perivitelline membrane binding potential after 48\u2009h (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05), whereas the other extenders showed results comparable to fresh semen. In summary, we demonstrate the effectiveness of the Botutainer device for preserving peccary semen at 5\u00b0C for up to 72\u2009h using TRIS, NUTRIXcell and PRIMXcell extenders.<\/jats:p>","DOI":"10.1111\/rda.70036","type":"journal-article","created":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T00:41:22Z","timestamp":1741740082000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Short\u2010Term Storage of Collared Peccary Semen in a Transport Container at 5\u00b0C Using Different Extenders"],"prefix":"10.1111","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2825-0258","authenticated-orcid":false,"given":"Rom\u00e1rio Parente","family":"dos Santos","sequence":"first","affiliation":[{"name":"Laboratory of Animal Germplasm Conservation, Department of Animal Sciences Federal University of the Semiarid Region\u2013UFERSA  Mossor\u00f3 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9204-9204","authenticated-orcid":false,"given":"Samara Sandy Jer\u00f4nimo","family":"Moreira","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Germplasm Conservation, Department of Animal Sciences Federal University of the Semiarid Region\u2013UFERSA  Mossor\u00f3 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1691-8815","authenticated-orcid":false,"given":"Luana Grasiele Pereira","family":"Bezerra","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Germplasm Conservation, Department of Animal Sciences Federal University of the Semiarid Region\u2013UFERSA  Mossor\u00f3 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuri Gon\u00e7alves","family":"Matos","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Germplasm Conservation, Department of Animal Sciences Federal University of the Semiarid Region\u2013UFERSA  Mossor\u00f3 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0174-491X","authenticated-orcid":false,"given":"Nayra Rachel Nascimento","family":"Luz","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Germplasm Conservation, Department of Animal Sciences Federal University of the Semiarid Region\u2013UFERSA  Mossor\u00f3 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-4626-5311","authenticated-orcid":false,"given":"Pedro Augusto Pinheiro","family":"Brito","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Germplasm Conservation, Department of Animal Sciences Federal University of the Semiarid Region\u2013UFERSA  Mossor\u00f3 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5894-0148","authenticated-orcid":false,"given":"Pedro Nacib","family":"Jorge\u2010Neto","sequence":"additional","affiliation":[{"name":"Reprocon Institute  Campo Grande Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6027-1062","authenticated-orcid":false,"given":"Alexandre Rodrigues","family":"Silva","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Germplasm Conservation, Department of Animal Sciences Federal University of the Semiarid Region\u2013UFERSA  Mossor\u00f3 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,3,7]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2016.03.007"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.21451\/1809\u20103000.RBRA2023.001"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439\u20100531.2006.00767.x"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1590\/1089\u20106891v17i118885"},{"key":"e_1_2_10_6_1","unstructured":"Botupharma. n.d.\u201cEquine Product Guide. 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