{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T07:23:49Z","timestamp":1780471429651,"version":"3.54.1"},"reference-count":26,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,3,8]],"date-time":"2025-03-08T00:00:00Z","timestamp":1741392000000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>This retrospective clinical study describes different techniques for transvaginal follicle aspiration in mares and compares results from 5 different commercial ovum pick\u2010up (OPU) clinics in which the same operator aspirated mares using different systems and equipment: Clinic 1 (<jats:italic>n<\/jats:italic>\u2009=\u200942 mares, two\u2010operator OPU technique, double\u2010lumen\u2010echogenic\u2010tip needle, and manual syringe\u2010assisted flushing of follicles), Clinic 2 (<jats:italic>n<\/jats:italic>\u2009=\u200928 mares, single\u2010operator\u2010OPU\u2010technique, double\u2010lumen\u2010echogenic\u2010tip needle, infusion pump controlled by foot\u2010pedal for follicle flushing), Clinic 3 (<jats:italic>n<\/jats:italic>\u2009=\u200918 mares, single\u2010operator\u2010OPU\u2010technique, double\u2010lumen\u2010echogenic\u2010tip needle, and manual syringe\u2010assisted flushing of follicles), Clinic 4 (<jats:italic>n<\/jats:italic>\u2009=\u200924 mares, single\u2010operator\u2010OPU\u2010technique, double\u2010lumen\u2010non\u2010echogenic\u2010tip needle, and manual syringe\u2010assisted flushing of follicles), and Clinic 5 (<jats:italic>n<\/jats:italic>\u2009=\u20099 mares, aspirated as in Clinic 1). The ease of performing OPU (visibility of needle tip and difficulty to hold ovary, probe, and needle) and the mean number of recovered oocytes were compared between clinics. The mean number of recovered oocytes per mare and oocytes per follicle for clinics 1\u20135 were 11.9\u2009\u00b1\u20094.6 and 64%, 13.5\u2009\u00b1\u20096.5 and 53%, 12.3\u2009\u00b1\u20094.2 and 54%, 9.5\u2009\u00b1\u20093.3 and 51%, and 19.9\u2009\u00b1\u20098.2 and 64%, respectively. The combined recovered oocyte per aspirated follicle was 10% higher in clinics with a 2\u2010operator technique (clinic 1 and 5, 63.63%) than in clinics with the single\u2010operator technique (clinics 2\u20134, 53.32%). The mean number of recovered oocytes was numerically greater in the clinics using the echogenic needle tip (11.9, 13.5, 12.3, and 19.9 oocytes per mare, for clinics 1, 2, 3, and 5, respectively) than in the clinic using the non\u2010echogenic needle tip; (9.5 oocytes per mare for clinic 4). The use of a plastic syringe or an infusion pump to flush follicles did not appear to affect the number of recovered oocytes or oocyte recovery rate (clinic 2 vs. 3). The left arm of the single operator (holding OPU probe and needle) experienced fatigue more frequently when mares were aspirated singly, than when the needle was managed by a second operator. In conclusion, the oocyte per follicle recovery was 10% lower in clinics using a single\u2010operator\u2010OPU\u2010technique compared with clinics using a two\u2010operator\u2010technique.<\/jats:p>","DOI":"10.1111\/rda.70043","type":"journal-article","created":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T18:35:52Z","timestamp":1741718152000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Transvaginal Follicle Aspiration in Mares: A Description of Different Techniques and Comparison of Results Across Different <scp>OPU<\/scp> Clinics"],"prefix":"10.1111","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6939-017X","authenticated-orcid":false,"given":"Juan","family":"Cuervo\u2010Arango","sequence":"first","affiliation":[{"name":"Equine Fertility Group, Facultad de Veterinaria Universidad Cardenal Herrera\u2010CEU, CEU Universities  Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denis","family":"Necchi","sequence":"additional","affiliation":[{"name":"Keros Embryo Transfer Center  Zonnebeke Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amber","family":"Clutton\u2010Brock","sequence":"additional","affiliation":[{"name":"Equibreed UK Ltd Lower Meadows Grange Lane  Beenham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magdalena","family":"Profaska","sequence":"additional","affiliation":[{"name":"Equibiotech sp. z o.o  Nowa Iwiczna Poland"},{"name":"Katedra Diagnostyki i Nauk Klinicznych, Wydzia\u0142 Medycyny Weterynaryjnej Uniwersytet Rolniczy Im. Hugona Ko\u0142\u0142\u0105taja w Krakowie  Krak\u00f3w Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"Crabtree","sequence":"additional","affiliation":[{"name":"Equine Reproductive Services (UK) ltd Trigger Castle  North Yorkshire UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony","family":"Claes","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences Utrecht University  Utrecht the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,8]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/evj.13028"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2022.04.019"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jevs.2019.01.015"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2019.06.020"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD19201"},{"key":"e_1_2_10_7_1","first-page":"313","volume-title":"The Equine Hospital Manual","author":"Donaldson L.","year":"2008"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/biolre\/ioac172"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2024.04.007"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/evj.12159"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2006.10.011"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(02)00771-9"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/evj.13054"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/biolreprod\/52.monograph_series1.243"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(91)90375-N"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2010.01624.x"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(87)90114-2"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod62.5.1402"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2010.01.013"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jevs.2020.103097"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.14625"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jevs.2020.103011"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-3306.2012.00606.x"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cveq.2013.11.008"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(88)80024-4"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.2460\/javma.22.06.0240"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1467-2987.2001.00047.x"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.70043","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,8,26]],"date-time":"2025-08-26T05:48:31Z","timestamp":1756187311000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.70043"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3]]},"references-count":26,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,3]]}},"alternative-id":["10.1111\/rda.70043"],"URL":"https:\/\/doi.org\/10.1111\/rda.70043","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3]]},"assertion":[{"value":"2024-10-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-28","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70043"}}