{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T16:53:33Z","timestamp":1758819213827,"version":"3.41.2"},"reference-count":52,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:00:00Z","timestamp":1747180800000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["315717\/2021\u20101"],"award-info":[{"award-number":["315717\/2021\u20101"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003947","name":"Universidad Nacional de La Plata","doi-asserted-by":"publisher","award":["V273"],"award-info":[{"award-number":["V273"]}],"id":[{"id":"10.13039\/501100003947","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Studies have demonstrated that many reproductive disorders can compromise the binding pattern of glycosidic residues, mainly in cows and sows. In the current study, the binding pattern of lectins was characterised and compared in normal and pathological ovaries of bitches. Fourteen biotinylated lectins (BSA\u20101B, Con\u2010A, DBA, LCA, PHA\u2010E, PHA\u2010L, PNA, RCA\u20101, SBA, SJA, STA, WGA, s\u2010WGA, UEA\u20101) were used. In ovaries without lesions, the predominant staining for RCA\u20101 was observed in the zona pellucida (ZP) of initial follicles, in the oocytes of primordial and primary follicles, and in the artery wall. For WGA, the reaction predominated in the mesothelium, in the ZP of secondary follicles, in the corpus luteum (CL) and in the oocyte of secondary follicles. Reactivity for STA was more pronounced in the mesothelium and ZP of developing follicles. The mesothelial cells and the CL were mainly marked for LCA. PHA\u2010L was stained in the mesothelium, CL, and connective stroma, while the lectin PHA\u2010E showed reactivity especially in the stroma, mesothelium, and artery wall. In the cystic ovaries, changes in the reaction of some lectins were noted in various structures adjacent to the cysts. RCA\u20101 staining was reduced in the ZP and oocyte of primordial, primary, and secondary follicles, while there was an increase of staining in endothelial cells. With WGA lectin, there was a reduction in CL and an increase in endothelial cells. Reactivity to STA was reduced in the ZP of primary and secondary follicles and increased in endothelial cells. LCA was also reduced in the CL and ZP of primary and secondary follicles. For the PHA\u2010L lectin, the staining was reduced in the mesothelial cells, CL, and ZP of the primary follicles, while for PHA\u2010E the reduction occurred in the stroma, oocyte of the primary follicles, and in the GC of the secondary follicles. In all neoplasms analysed (dysgerminoma, granulosa cell tumour, adenocarcinoma), there was also a reduction in reactivity to various lectins, both in the neoplastic cells compared to the cells of origin and in the adjacent non\u2010neoplastic ovarian tissue. The data from the current study suggest that the changes in ovarian glycosidic residues observed in cysts and neoplasms may contribute to infertility in bitches.<\/jats:p>","DOI":"10.1111\/rda.70075","type":"journal-article","created":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T11:39:31Z","timestamp":1747222771000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Lectin\u2010Histochemical Pattern on the Cystic and Neoplastic Ovaries of Bitches"],"prefix":"10.1111","volume":"60","author":[{"given":"Juliano P.","family":"Terra","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Diagn\u00f3stico Patol\u00f3gico Veterin\u00e1rio (LDPV) Universidade de Bras\u00edlia (UnB)  Bras\u00edlia Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thiago B.","family":"Almeida","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Diagn\u00f3stico Patol\u00f3gico Veterin\u00e1rio (LDPV) Universidade de Bras\u00edlia (UnB)  Bras\u00edlia Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucas P. O.","family":"Rezende","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Diagn\u00f3stico Patol\u00f3gico Veterin\u00e1rio (LDPV) Universidade de Bras\u00edlia (UnB)  Bras\u00edlia Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9459-138X","authenticated-orcid":false,"given":"Claudio G.","family":"Barbeito","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Veterinarias (FCV) Universidad Nacional de La Plata (UNLP)  La Plata Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francisco","family":"Acu\u00f1a","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Veterinarias (FCV) Universidad Nacional de La Plata (UNLP)  La Plata Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guilherme R.","family":"Blume","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio HistoPato An\u00e1lise Anatomopatol\u00f3gica  Bras\u00edlia Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R\u00f4mulo S. 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