{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:43:50Z","timestamp":1753879430322,"version":"3.41.2"},"reference-count":22,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,5,15]],"date-time":"2025-05-15T00:00:00Z","timestamp":1747267200000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Using a synchronisation protocol based on estradiol esters and prostaglandin, established in cattle since the 1970s, we implemented 24 or 48\u2010h intervals between hormone administration and timed artificial insemination (TAI). This study compared two short\u2010duration TAI protocols using estradiol benzoate (EB) and cypionate (EC) as ovulation inducers. A total of 172 Girolando female cows were selected after ovarian ultrasonographic evaluation and received 500\u2009\u03bcg of prostaglandin F2\u03b1 (PGF) at the start of the treatment (D0). The inclusion criterion for females in the study was the presence of a corpus luteum measuring \u2265\u200914\u2009mm and at least one follicle with a diameter between 8 and 20\u2009mm. The cows were randomly sorted into two treatment groups. The EB group received 2\u2009mg of EB 24\u2009h after PGF (D1), and TAI was performed 24\u2009h later (D2). The EC group received 2\u2009mg estradiol cypionate simultaneously with PGF, and TAI was performed 48\u2009h later. Pregnancy was diagnosed 30\u2009days after AI using transrectal ultrasonography. Experiment I evaluated follicular dynamics in 18 females (EB: <jats:italic>n<\/jats:italic>\u2009=\u20099; EC: <jats:italic>n<\/jats:italic>\u2009=\u20099) by analysing the follicular growth rate, pre\u2010ovulatory follicle (POF) diameter, ovulation timing and ovulation rate. No statistically significant differences were observed (<jats:italic>p<\/jats:italic>\u2009&gt;\u20090.05), with a 77.8% ovulation rate in both treatments. Experiment II compared the conception rates in 172 females (EB: <jats:italic>n<\/jats:italic>\u2009=\u200985; EC: <jats:italic>n<\/jats:italic>\u2009=\u200987). The overall conception rate was 30.2%, with no significant difference between the treatments (EB: 27.1%; EC: 33.3%). However, cows with a body condition score &lt;\u20093.0 tended to have higher conception rates with EC than with EB (39.1% vs. 18.2%; <jats:italic>p<\/jats:italic>\u2009=\u20090.06). Cows with POF \u2265\u200910\u2009mm at D0 had significantly higher conception rates with EC than with EB (48.3% vs. 29.0%; <jats:italic>p<\/jats:italic>\u2009=\u20090.03). Both protocols were equally effective; however, EC was more advantageous for cows with a POF \u2265\u200910\u2009mm at treatment initiation.<\/jats:p>","DOI":"10.1111\/rda.70076","type":"journal-article","created":{"date-parts":[[2025,5,16]],"date-time":"2025-05-16T05:59:50Z","timestamp":1747375190000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Efficacy of Short\u2010Term Timed Artificial Insemination Protocols With Estradiol Esters and <scp>PGF2\u03b1<\/scp> in Girolando Cows"],"prefix":"10.1111","volume":"60","author":[{"given":"Mariana","family":"dos Santos Dutra Okada","sequence":"first","affiliation":[{"name":"Universidade Federal Rural do Rio de Janeiro  Serop\u00e9dica Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lara Nogueira","family":"Silenciato","sequence":"additional","affiliation":[{"name":"Centro Universit\u00e1rio de Barra Mansa  Barra Mansa Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samuel Rodrigues Bonamichi","family":"do Couto","sequence":"additional","affiliation":[{"name":"Universidade Federal Rural do Rio de Janeiro  Serop\u00e9dica Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joaquim Esquerdo","family":"Ferreira","sequence":"additional","affiliation":[{"name":"UNIFAA Centro Universit\u00e1rio de Valen\u00e7a  Valen\u00e7a Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9790-4764","authenticated-orcid":false,"given":"Marco Roberto Bourg","family":"de Mello","sequence":"additional","affiliation":[{"name":"Universidade Federal Rural do Rio de Janeiro  Serop\u00e9dica Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,5,15]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_10_2_1","DOI":"10.4322\/rbcv.2016.038"},{"doi-asserted-by":"publisher","key":"e_1_2_10_3_1","DOI":"10.1016\/j.theriogenology.2019.09.024"},{"doi-asserted-by":"publisher","key":"e_1_2_10_4_1","DOI":"10.1016\/S0093\u2010691X(01)00646\u2010X"},{"doi-asserted-by":"publisher","key":"e_1_2_10_5_1","DOI":"10.1111\/j.1365\u20102826.1994.tb00590.x"},{"doi-asserted-by":"publisher","key":"e_1_2_10_6_1","DOI":"10.1016\/j.anireprosci.2007.12.004"},{"doi-asserted-by":"publisher","key":"e_1_2_10_7_1","DOI":"10.3168\/jds.S0022\u20100302(98)75758\u20103"},{"doi-asserted-by":"publisher","key":"e_1_2_10_8_1","DOI":"10.1590\/1984\u20103143\u2010AR2023\u20100123"},{"doi-asserted-by":"publisher","key":"e_1_2_10_9_1","DOI":"10.1590\/S1519\u201099402015000400019"},{"doi-asserted-by":"publisher","key":"e_1_2_10_10_1","DOI":"10.1095\/biolreprod66.6.1689"},{"doi-asserted-by":"publisher","key":"e_1_2_10_11_1","DOI":"10.1262\/jrd.2012\u2010117"},{"doi-asserted-by":"publisher","key":"e_1_2_10_12_1","DOI":"10.1016\/S0093\u2010691X(00)00370\u20108"},{"doi-asserted-by":"publisher","key":"e_1_2_10_13_1","DOI":"10.1016\/j.anireprosci.2015.05.006"},{"doi-asserted-by":"publisher","key":"e_1_2_10_14_1","DOI":"10.3168\/jds.2009\u20102930"},{"doi-asserted-by":"publisher","key":"e_1_2_10_15_1","DOI":"10.3168\/jds.2024\u201025498"},{"doi-asserted-by":"publisher","key":"e_1_2_10_16_1","DOI":"10.1016\/j.cvfa.2023.08.008"},{"doi-asserted-by":"publisher","key":"e_1_2_10_17_1","DOI":"10.1007\/BF02267642"},{"doi-asserted-by":"publisher","key":"e_1_2_10_18_1","DOI":"10.11606\/issn.1678\u20104456.bjvras.2019.155078"},{"doi-asserted-by":"publisher","key":"e_1_2_10_19_1","DOI":"10.1016\/j.theriogenology.2018.04.031"},{"doi-asserted-by":"publisher","key":"e_1_2_10_20_1","DOI":"10.1016\/S0093\u2010691X(03)00042\u20106"},{"doi-asserted-by":"publisher","key":"e_1_2_10_21_1","DOI":"10.3168\/jds.S0022\u20100302(06)72333\u20105"},{"doi-asserted-by":"publisher","key":"e_1_2_10_22_1","DOI":"10.1016\/j.theriogenology.2024.03.005"},{"doi-asserted-by":"publisher","key":"e_1_2_10_23_1","DOI":"10.1111\/j.1365\u20102885.1990.tb00745.x"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.70076","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,28]],"date-time":"2025-05-28T05:47:08Z","timestamp":1748411228000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.70076"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5]]},"references-count":22,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2025,5]]}},"alternative-id":["10.1111\/rda.70076"],"URL":"https:\/\/doi.org\/10.1111\/rda.70076","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"type":"print","value":"0936-6768"},{"type":"electronic","value":"1439-0531"}],"subject":[],"published":{"date-parts":[[2025,5]]},"assertion":[{"value":"2025-03-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-05-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-05-15","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70076"}}