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N\u2010acetylcysteine (NAC), a derivative of cysteine, participates in glutathione (GSH) metabolism and stimulates glutathione synthesis. However, a clear understanding of the effect of NAC on sheep oocytes remains unknown. In this study, we investigated NAC's impact on the maturation of sheep oocytes, and the results revealed that the maturation rate, and subsequently the cleavage and blastocyst formation, were significantly enhanced by incubation with 1\u2009mM NAC. The GSH and Ca<jats:sup><jats:bold>2<\/jats:bold>+<\/jats:sup> levels increased, and the cortical granules were significantly elevated, whereas the reactive oxygen species levels were significantly reduced in the 1\u2009mM NAC\u2010treated group. Additionally, the number of inner cell masses was significantly increased. The findings of this study support the hypothesis that NAC increases oocyte maturation rate by protecting them from oxidative stress damage. These discoveries provide a new approach for improving the efficiency of in\u00a0vitro production of sheep embryos.<\/jats:p>","DOI":"10.1111\/rda.70084","type":"journal-article","created":{"date-parts":[[2025,9,15]],"date-time":"2025-09-15T07:04:02Z","timestamp":1757919842000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["N\u2010Acetylcysteine Promotes the Maturation of Sheep Oocytes and Embryo Development In\u00a0Vitro"],"prefix":"10.1111","volume":"60","author":[{"given":"Can","family":"Li","sequence":"first","affiliation":[{"name":"Key Laboratory of Livestock Biology, College of Veterinary Medicine Northwest A&amp;F University  Yangling Shaanxi Province People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingbing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Livestock Biology, College of Veterinary Medicine Northwest A&amp;F 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