{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,28]],"date-time":"2025-08-28T10:40:02Z","timestamp":1756377602333,"version":"3.44.0"},"reference-count":22,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,7,28]],"date-time":"2025-07-28T00:00:00Z","timestamp":1753660800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000646","name":"Japan Society for the Promotion of Science London","doi-asserted-by":"publisher","award":["JP18K05957"],"award-info":[{"award-number":["JP18K05957"]}],"id":[{"id":"10.13039\/501100000646","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>This study investigated the effects of multiple treatments of estradiol benzoate (EB) prior to the induction of luteal regression on the efficiency of artificial lactation induction and milk yield in pseudopregnant sows. Pseudopregnant females induced by treatment with 30\u2009mg estradiol dipropionate (EDP) were administered 2\u2009mg (Group EB 2\u2009mg, <jats:italic>n<\/jats:italic>\u2009=\u20095) or 3\u2009mg (Group EB 3\u2009mg, <jats:italic>n<\/jats:italic>\u2009=\u20096) EB on Days\u221210, \u22127, and \u22124 (Day 0\u2009=\u2009the day of prostaglandin F<jats:sub>2\u03b1<\/jats:sub> [PGF<jats:sub>2\u03b1<\/jats:sub>] treatment). Six pseudopregnant sows (Group EDP) received 5\u2009mg EDP on Day \u221210.5\u2009\u00b1\u20091.6. The induction efficiency of artificial lactation, collected milk volume and immunoglobulin concentrations in the milk obtained from sows after PGF<jats:sub>2\u03b1<\/jats:sub> treatment were analysed. The dosage of EB treatment before PGF<jats:sub>2\u03b1<\/jats:sub> administration had no significant effect on either the induction rate of artificial lactation or the milk yield during the experimental period. Harvested milk volumes from EB\u2010treated pseudopregnant sows were higher than those in Group EDP. The immunoglobulin levels in harvested milk from artificial lactating sows did not differ between the EB\u2010treated groups. In conclusion, multiple EB treatments prior to PGF<jats:sub>2\u03b1<\/jats:sub> administration increased milk production in pseudopregnant sows but did not affect the efficiency of artificial lactation induction.<\/jats:p>","DOI":"10.1111\/rda.70105","type":"journal-article","created":{"date-parts":[[2025,7,28]],"date-time":"2025-07-28T09:15:05Z","timestamp":1753694105000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Multiple Estradiol Benzoate Treatment Enhances Milk Production on Induction of Artificial Lactation in Pseudopregnant Pigs"],"prefix":"10.1111","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-3230-4580","authenticated-orcid":false,"given":"Michiko","family":"Noguchi","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine Azabu University  Sagamihara Kanagawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuta","family":"Takamori","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine Azabu University  Sagamihara Kanagawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mayuki","family":"Asano","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine Azabu University  Sagamihara Kanagawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4612-6624","authenticated-orcid":false,"given":"Yosuke","family":"Sasaki","sequence":"additional","affiliation":[{"name":"Department of Agriculture Meiji University  Kawasaki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koji","family":"Yoshioka","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine Azabu University  Sagamihara Kanagawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,7,28]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"157","article-title":"The Transfer of Immunoglobulins IgG, IgA and IgM From Serum to Colostrum and Milk in the Sow","volume":"24","author":"Bourne F. J.","year":"1973","journal-title":"Immunology"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod45.3.478"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.14348"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0739\u20107240(86)90016\u20100"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1051\/rnd:19780410"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1017\/S175173110700016X"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/jas\/sky395"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022\u20100302(72)85664\u20139"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022\u20100302(82)82293\u20135"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.domaniend.2009.04.002"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.domaniend.2024.106851"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165\u20100378(92)90037\u20105"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1017\/s1751731119000521"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/asj.13815"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.12\u20100022"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.14571"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.2020\u2010034"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.livsci.2012.03.010"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0303\u20107207(95)03519\u2010D"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.23571"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1751731114000950"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.135.3.1241"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.70105","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,8,28]],"date-time":"2025-08-28T10:14:55Z","timestamp":1756376095000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.70105"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,28]]},"references-count":22,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2025,8]]}},"alternative-id":["10.1111\/rda.70105"],"URL":"https:\/\/doi.org\/10.1111\/rda.70105","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"type":"print","value":"0936-6768"},{"type":"electronic","value":"1439-0531"}],"subject":[],"published":{"date-parts":[[2025,7,28]]},"assertion":[{"value":"2025-05-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-28","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70105"}}