{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T16:12:00Z","timestamp":1791994320843,"version":"4.4.0"},"reference-count":25,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,7,31]],"date-time":"2025-07-31T00:00:00Z","timestamp":1753920000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005071","name":"Shiraz University","doi-asserted-by":"publisher","award":["1GCB4M369962"],"award-info":[{"award-number":["1GCB4M369962"]}],"id":[{"id":"10.13039\/501100005071","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Cold storage is a preferred method for short\u2010term preservation of ram spermatozoa due to its superior fertility rates compared to cryopreservation. This study aimed to optimise the conditions for ram sperm preservation by evaluating the effects of osmolarity and egg yolk (EY) concentration in a syringe\u2010filtered extender. In part A, semen samples were extended with solutions of varying osmolarities (330, 360, 390 and 420\u2009mOsm\/kg water). The 390\u2009mOsm\/kg solution demonstrated the best preservation of sperm viability, functional integrity and motility over 96\u2009h of storage. In part B, different EY concentrations (10%, 15%, 20%, 25% and 30%) were assessed at the optimal osmolarity (390\u2009mOsm\/kg). A 20% EY concentration provided the most effective protection of sperm DNA, acrosome integrity and membrane functionality. Excessive EY levels (&gt;\u200920%) negatively impacted sperm viability and induced higher lipid peroxidation. These findings emphasise the importance of balancing osmolarity and EY concentration for efficient cold storage of ram spermatozoa, enhancing the potential success of artificial insemination programs in sheep breeding.<\/jats:p>","DOI":"10.1111\/rda.70106","type":"journal-article","created":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T05:46:28Z","timestamp":1754027188000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Optimising Filtered Egg Yolk Extenders for Long\u2010Term Cold Preservation of Ram Sperm: Effects of Osmolarity and Egg Yolk Concentration"],"prefix":"10.1111","volume":"60","author":[{"given":"Mohammad Hassan Nategh","family":"Ahmadi","sequence":"first","affiliation":[{"name":"School of Veterinary Medicine Shiraz University  Shiraz Fars Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdolah","family":"Mirzaei","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, School of Veterinary Medicine Shiraz University  Shiraz Fars Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8021-429X","authenticated-orcid":false,"given":"Mohammad Reza","family":"Divar","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, School of Veterinary Medicine Shiraz University  Shiraz Fars Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatemeh","family":"Derafshani","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine Shiraz University  Shiraz Fars Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,31]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2004.01.013"},{"key":"e_1_2_9_3_1","unstructured":"Acharya M.2017.\u201cImprovement in Simple Artificial Insemination in Sheep Using Chilled Extended Semen and Consideration of Fertility in Ewes.\u201d"},{"issue":"1","key":"e_1_2_9_4_1","first-page":"14","article-title":"Effect of Semen Extender and Storage Temperature on Motility of Ram Spermatozoa","volume":"8","author":"Acharya M.","year":"2019","journal-title":"Advances in Reproductive Sciences"},{"issue":"2","key":"e_1_2_9_5_1","first-page":"33","article-title":"Effects of Extender Osmolarity, Cooling Rate, Dilution Rate and Glycerol Addition Time on Post\u2010Thaw Ram Semen Characteristics and Fertilization","volume":"36","author":"Ak K.","year":"2010","journal-title":"\u0130stanbul \u00dcniversitesi Veteriner Fak\u00fcltesi Dergisi"},{"issue":"3","key":"e_1_2_9_6_1","first-page":"431","article-title":"Analysis of Awassi Sperm Motility in Two Media at Different Levels of Temperature, pH and Osmolality","volume":"8","author":"Alomar M.","year":"2018","journal-title":"Iranian Journal of Applied Animal Science"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.5958\/2277-940X.2016.00074.7"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2007.07.003"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/00288230709510285"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells11182845"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.14284"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.4236\/ojas.2021.113033"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-73.4.715"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2018.10.022"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD9930613"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4320(96)01544-8"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.12225"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.12681\/jhvms.18014"},{"issue":"2","key":"e_1_2_9_19_1","first-page":"168","article-title":"Effect of Different Levels of Egg Yolk on Ram Sperm Coating and Preserving at 5\u00b0C","volume":"15","author":"Rajabi\u2010Toustani R.","year":"2014","journal-title":"Iranian Journal of Veterinary Research"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani12030244"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/7378379"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2023.1339840"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2022.1013533"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.2478\/fv-2024-0025"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2011.11.003"},{"issue":"1","key":"e_1_2_9_26_1","first-page":"18","article-title":"Effects of Proportion of Egg Yolk and Preservation Time on Chilled Semen From Indigenous Rams","volume":"1","author":"Zohara B. 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