{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T14:10:08Z","timestamp":1759155008514,"version":"3.44.0"},"reference-count":39,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,9,9]],"date-time":"2025-09-09T00:00:00Z","timestamp":1757376000000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The present study was undertaken to assess the effect of kisspeptin supplementation (0.0, 5.0, 10.0, 15.0, 20.0 and 40.0\u2009\u03bcM) in cryodiluent on freezability and in\u00a0vivo fertility of buffalo bull spermatozoa. Twenty\u2010four semen ejaculates were collected using an artificial vagina from four Murrah buffalo bulls. Ejaculates having &gt;\u200970% sperm motility, &lt;\u200925% morphological abnormalities and &gt;\u2009500 million\/ml concentration were diluted with Tris\u2010egg yolk extender containing 0.0 (control), 5.0, 10.0, 15.0, 20.0 and 40.0\u2009\u03bcM kisspeptin and cryopreserved following established protocol. The CASA\u2010based progressive motility and viability were higher (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) in cryodiluent containing 20.0\u2009\u03bcM of kisspeptin in comparison to other experimental concentrations and control. Addition of kisspeptin at 20.0 and 40.0\u2009\u03bcM in extender exhibited higher (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) CASA\u2010based total motility and plasma membrane integrity. Regarding CASA\u2010based sperm kinematics, supplementation of kisspeptin (20.0\u2009\u03bcM) in extender improved (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) VCL and VAP as compared to other tested concentrations and control. The acrosome integrity revealed no difference (<jats:italic>p<\/jats:italic>\u2009&gt;\u20090.05) in all the experimental extenders compared to control. The MDA levels were lower (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) in cryodiluent supplemented with kisspeptin (20.0\u2009\u03bcM) than in other concentrations and control. Sixty artificial inseminations were performed with the post\u2010thaw semen having the most effective dose of kisspeptin (20.0\u2009\u03bcM) and control (30 inseminations each). The conception rates were recorded to be higher (<jats:italic>p<\/jats:italic>\u2009&gt;\u20090.05) using post\u2010thaw semen containing kisspeptin (20.0\u2009\u03bcM; 43.3%) as compared to control (33.3%). In conclusion, complementing the cryodiluent with 20.0\u2009\u03bcM kisspeptin improved the quality and in\u00a0vivo fertility of cryopreserved Murrah buffalo bull semen.<\/jats:p>","DOI":"10.1111\/rda.70124","type":"journal-article","created":{"date-parts":[[2025,9,9]],"date-time":"2025-09-09T08:36:17Z","timestamp":1757406977000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effect of Kisspeptin Fortification in Freezing Media on Post\u2010Thaw Quality and Fertility of Buffalo Bull Semen"],"prefix":"10.1111","volume":"60","author":[{"given":"Balkar","family":"Singh","sequence":"first","affiliation":[{"name":"Department of Veterinary Gynaecology and Obstetrics Guru Angad Dev Veterinary and Animal Sciences University  Ludhiana Punjab India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2164-764X","authenticated-orcid":false,"given":"Ashwani Kumar","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Veterinary Gynaecology and Obstetrics Guru Angad Dev Veterinary and Animal Sciences University  Ludhiana Punjab India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5884-0414","authenticated-orcid":false,"given":"Pooja","family":"Devi","sequence":"additional","affiliation":[{"name":"Department of Veterinary Physiology and Biochemistry Guru Angad Dev Veterinary and Animal Sciences University  Ludhiana Punjab India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ajeet","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Teaching Veterinary Clinical Complex Guru Angad Dev Veterinary and Animal Sciences University  Ludhiana Punjab India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Narinder","family":"Singh","sequence":"additional","affiliation":[{"name":"Directorate of Livestock Farms Guru Angad Dev Veterinary and Animal Sciences University  Ludhiana Punjab India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Navdeep Singh","family":"Ratta","sequence":"additional","affiliation":[{"name":"Directorate of Livestock Farms Guru Angad Dev Veterinary and Animal Sciences University  Ludhiana Punjab India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5298-7781","authenticated-orcid":false,"given":"Prahlad","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Teaching Veterinary Clinical Complex Guru Angad Dev Veterinary and Animal Sciences University  Ludhiana Punjab India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,9,9]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smallrumres.2019.09.004"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2019.10.039"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2005.05328.x"},{"volume-title":"The Association of Seminal Inflammatory Markers, Standard Semen Parameters and Sperm Kinematics With Sperm DNA Fragmentation. (Doctoral Dissertation)","year":"2020","author":"Aghazarian A.","key":"e_1_2_10_5_1"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2021.106761"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1387\/ijdb.130146ja"},{"issue":"4","key":"e_1_2_10_8_1","first-page":"307","article-title":"Effects of Kisspeptin Incubation on the Mature Mouse Testicular Sperms Quality: An Experimental Study","volume":"20","author":"Akmali M.","year":"2022","journal-title":"International Journal of Reproductive Biomedicine"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.37940\/AJVS.2019.12.1.9"},{"issue":"4","key":"e_1_2_10_10_1","first-page":"159","article-title":"Effect of Non\u2010Enzymatic Antioxidants in Extender on Post\u2010Thaw Quality of Buffalo (Bubalus Bubalis) Bull Spermatozoa","volume":"28","author":"Andrabi S. M. H.","year":"2008","journal-title":"Pakistan Veterinary Journal"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.13761"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(78)52032-6"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox10010092"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.14564"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2008.06.016"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.13057"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1009420432234"},{"issue":"5","key":"e_1_2_10_18_1","first-page":"354","article-title":"Additives Used in Semen Preservation in Animals: A Short Review","volume":"6","author":"Hussain M.","year":"2018","journal-title":"International Journal of Chemical Studies"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2025.105202"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2305-0500(14)60028-2"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2008.10.002"},{"issue":"2","key":"e_1_2_10_22_1","first-page":"198","article-title":"Effect of Pre\u2010Incubation of Cryopreserved Sperm With Either Kisspeptin or Glutathione to Mitigate Freeze\u2010Thaw Damage","volume":"48","author":"Kermani T.","year":"2023","journal-title":"Iranian Journal of Medical Sciences"},{"issue":"4","key":"e_1_2_10_23_1","first-page":"579","article-title":"Possible Role of Kisspeptin in Regulation of Motility Spectrum of Buffalo Bull Spermatozoa: A Preliminary Study","volume":"41","author":"Khan L.","year":"2021","journal-title":"Pakistan Veterinary Journal"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2024.107512"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1089\/bio.2019.0037"},{"key":"e_1_2_10_26_1","unstructured":"National Dairy Development Board.2024.Annual Report 2023\u20132024.https:\/\/www.nddb.coop\/."},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.56093\/ijans.v86i9.61570"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2605.2011.01177.x"},{"issue":"10","key":"e_1_2_10_29_1","first-page":"766","article-title":"Hypo\u2010Osmotic Swelling Test (HOST) amd Its Response in Fresh and Freeze\u2010Thawed Semen","volume":"69","author":"Prasad J. K.","year":"1999","journal-title":"Indian Journal of Animal Sciences"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2005.02.035"},{"issue":"6","key":"e_1_2_10_31_1","first-page":"737","article-title":"Oxidative Stress and Male Infertility: From Research Bench to Clinical Practice","volume":"23","author":"Saleh R. A.","year":"2002","journal-title":"Andrology"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477-7827-2-12"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21082958"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.5713\/ab.21.0072"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.12590"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.3109\/09513591003686395"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0967199423000412"},{"issue":"1","key":"e_1_2_10_38_1","first-page":"27","article-title":"Reproductive Wastage Rate of Crossbred Dairy Bulls With Reference to Level of Exotic Inheritance and Number of Breed Components","volume":"27","author":"Tyagi S.","year":"2006","journal-title":"Indian Journal of Animal Reproduction"},{"key":"e_1_2_10_39_1","first-page":"524","volume-title":"Semen Characteristics and Artificial Insemination in the Buffalo","author":"Vale W. G.","year":"2014"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2005.11.003"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.70124","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T13:35:25Z","timestamp":1759152925000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.70124"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9]]},"references-count":39,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2025,9]]}},"alternative-id":["10.1111\/rda.70124"],"URL":"https:\/\/doi.org\/10.1111\/rda.70124","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"type":"print","value":"0936-6768"},{"type":"electronic","value":"1439-0531"}],"subject":[],"published":{"date-parts":[[2025,9]]},"assertion":[{"value":"2025-06-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-03","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70124"}}