{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T19:25:20Z","timestamp":1770492320987,"version":"3.49.0"},"reference-count":14,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T00:00:00Z","timestamp":1758672000000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Leptospirosis is a zoonotic disease caused by pathogenic bacteria of the genus <jats:italic>Leptospira<\/jats:italic>. A lesser\u2010known form, equine genital leptospirosis (EGL), has been identified as a chronic and often silent infection involving the colonisation of the mare's genital tract. Despite its potential impact, EGL remains underdiagnosed and poorly understood, particularly in its association with reproductive inefficiency. This study showed the presence of <jats:italic>Leptospira<\/jats:italic> spp. DNA by <jats:italic>lip<\/jats:italic>L32\u2010PCR in the genital tract of mares with a history of reproductive disturbances. Cervicovaginal mucus samples were collected from 120 adult mares exhibiting recent reproductive problems. Results showed that 30 (25%) of the mares tested positive for <jats:italic>Leptospira<\/jats:italic> DNA. Among these 30 positive cases, 23.3% had experienced abortions, 3.3% had stillbirths, 53.3% showed placental alterations, and 36.6% were subfertile. These findings suggest a possible association between EGL and reproductive disorders in mares. The high detection rate of <jats:italic>Leptospira<\/jats:italic> DNA in genital samples reinforces the need for increased awareness and improved diagnostic efforts.<\/jats:p>","DOI":"10.1111\/rda.70125","type":"journal-article","created":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T07:05:52Z","timestamp":1758697552000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Detection of Pathogenic <i>Leptospira<\/i><scp>DNA<\/scp> in Cervicovaginal Mucus of Mares With Reproductive Disorders in a Brazilian Herd"],"prefix":"10.1111","volume":"60","author":[{"given":"Daiany","family":"Motta","sequence":"first","affiliation":[{"name":"Laboratory of Veterinary Bacteriology, Biomedical Institute Federal Fluminense University  Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juliana","family":"Pedrosa","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Bacteriology, Biomedical Institute Federal Fluminense University  Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2434-8728","authenticated-orcid":false,"given":"Walter","family":"Lilenbaum","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Bacteriology, Biomedical Institute Federal Fluminense University  Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,9,24]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2015.07.009"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jevs.2024.105099"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2024.10.020"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1590\/0001\u20103765202220201026"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2022.08.029"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2020.108869"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/evj.13069"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2024.1346713"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2015.06.014"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096\u2010019\u201003797\u20104"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/evj.13022"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.17184"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-323-44329-6.00016-4"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2009.03.014"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.70125","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T13:35:09Z","timestamp":1759152909000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.70125"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9]]},"references-count":14,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2025,9]]}},"alternative-id":["10.1111\/rda.70125"],"URL":"https:\/\/doi.org\/10.1111\/rda.70125","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9]]},"assertion":[{"value":"2025-06-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-11","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70125"}}