{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T19:25:05Z","timestamp":1791573905689,"version":"4.3.4"},"reference-count":40,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T00:00:00Z","timestamp":1758844800000},"content-version":"vor","delay-in-days":25,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>This study evaluated the clinical efficacy of aglepristone for the management of canine pyometra\u2014either as a sole therapy or in combination with different antibiotic regimens\u2014and investigated the association between aglepristone dosing and the occurrence of vaginal hyperplasia during the subsequent estrous cycle. Eighty\u2010seven intact bitches diagnosed with pyometra were enrolled and divided into two experiments. In Experiment I, 57 bitches were randomly assigned to three treatment groups (<jats:italic>n<\/jats:italic> =\u200919\/group): (1) AGL, aglepristone alone; (2) AGL\u2010CEF, aglepristone plus ceftriaxone; and (3) AGL\u2010CEF\u2010MET, aglepristone plus ceftriaxone and metronidazole. Clinical examinations and haematological and biochemical profiles were assessed every 48 h for 15\u2009days. In Experiment II, 30 bitches were treated with aglepristone using two protocols: AGL 4X (10 mg\/kg s.c. on Days 0, 1, 8 and 15) or AGL 2X (10 mg\/kg s.c. on Days 0 and 1). Incidence and severity of vaginal hyperplasia, as well as post\u2010treatment reproductive performance, were evaluated during the subsequent oestrus. All three treatment protocols achieved clinical resolution of pyometra\u2014including the aglepristone\u2010only group\u2014with normalisation of leukocyte counts and serum biochemical markers. In Experiment II, vaginal hyperplasia occurred in 53.3% of bitches in the AGL 4X group versus 7% in the AGL 2X group. Grade III hyperplasia was associated with pyometra recurrence and reduced pregnancy rates. Overall pregnancy rate in Experiment II was 80%. These findings indicate that aglepristone is an effective, fertility\u2010preserving treatment for canine pyometra, even when administered without systemic antibiotics. However, high\u2010dose or prolonged administration may induce oestrogen\u2010mediated adverse effects such as vaginal hyperplasia and disease recurrence. A reduced dosing regimen appears safer while maintaining equivalent clinical and reproductive efficacy.<\/jats:p>","DOI":"10.1111\/rda.70127","type":"journal-article","created":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T10:50:26Z","timestamp":1758883826000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Antibiotic\u2010Free Management of Canine Pyometra With Aglepristone: Endocrine Effects and Post\u2010Treatment Vaginal Hyperplasia"],"prefix":"10.1111","volume":"60","author":[{"given":"Thiago Vaz","family":"Lopes","sequence":"first","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria Universidade Federal Do Acre  Rio Branco Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3700-1811","authenticated-orcid":false,"given":"Luiz Ernandes","family":"Kozicki","sequence":"additional","affiliation":[{"name":"Pontif\u00edcia Universidade cat\u00f3lica Do Paran\u00e1  Curitiba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro Henrique Lomba","family":"de Lima","sequence":"additional","affiliation":[{"name":"Pontif\u00edcia Universidade cat\u00f3lica Do Paran\u00e1  Curitiba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8977-9740","authenticated-orcid":false,"given":"Nat\u00e1lia Siqueira","family":"de Lara","sequence":"additional","affiliation":[{"name":"Universidade Federal Do Paran\u00e1  Curitiba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mayara","family":"Silvestri","sequence":"additional","affiliation":[{"name":"Universidade Federal Do Paran\u00e1  Curitiba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduarda Stankiwich","family":"Vaz","sequence":"additional","affiliation":[{"name":"Universidade Federal Do Paran\u00e1  Curitiba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafaela","family":"Morettini e Castella","sequence":"additional","affiliation":[{"name":"Universidade Federal Do Paran\u00e1  Curitiba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciane Maria","family":"Laskoski","sequence":"additional","affiliation":[{"name":"Universidade Federal Do Paran\u00e1  Curitiba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando Andrade","family":"Souza","sequence":"additional","affiliation":[{"name":"Universidade Federal Do Paran\u00e1  Curitiba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,9,26]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2021.110299"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvsm.2008.10.001"},{"issue":"21","key":"e_1_2_9_4_1","first-page":"1170","article-title":"Hiperplasia vaginal em cadela: relato de caso","volume":"11","author":"Borges T. 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