{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T07:23:46Z","timestamp":1767597826110,"version":"3.46.0"},"reference-count":17,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T00:00:00Z","timestamp":1759104000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100010719","name":"Pakistan Agricultural Research Council","doi-asserted-by":"publisher","award":["AS\u2010035"],"award-info":[{"award-number":["AS\u2010035"]}],"id":[{"id":"10.13039\/501100010719","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Efficient use of frozen\u2010thawed semen in sheep is limited by low fertility following cervical insemination (CAI), whereas laparoscopic insemination (LAI) offers higher success but requires specialised skills and equipment. The current study aimed to evaluate the fertility outcomes of two artificial insemination techniques, laparoscopic and transcervical, in Lohi sheep using frozen\u2010thawed semen. Lohi ewes (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200944) were synchronised through progestin\u2010sponges (day 0\u2013day 11), administering PGF2\n                    <jats:sub>\u03b1<\/jats:sub>\n                    (d\u2010cloprostenol 75\u2009\u03bcg\/mL; i.m @ 75\u2009\u03bcg\/ewe) on day 09 and eCG (i.m @ 300\u2009IU\/ewe) on day 11. Synchronised ewes (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200944) were divided into two groups for artificial insemination (AI) procedures: cervical (CAI;\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200924; sperm concentratio\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u2009200\u2009million\/dose) and laparoscopic (LAI;\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200920; sperm concentration\u2009=\u200950\u2009million\/dose). All ewes were inseminated at 54\u2009h post\u2010sponge removal. Results of the experiment revealed that LAI demonstrated higher pregnancy rates (65%) than CAI (33.3%) (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.05). However, no significant differences were observed in prolificacy or twinning rates between the two techniques (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.11). In conclusion, LAI as compared to CAI is a superior method of insemination for achieving optimum pregnancy rates in estrous synchronised Lohi ewes.\n                  <\/jats:p>","DOI":"10.1111\/rda.70129","type":"journal-article","created":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T05:30:11Z","timestamp":1759210211000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Fertility Outcomes in Lohi Sheep: A Comparative Study of Laparoscopic Versus Trans\u2010Cervical\n                    <scp>AI<\/scp>\n                    With Frozen\u2010Thawed Semen Under Subtropical Conditions"],"prefix":"10.1111","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4624-1531","authenticated-orcid":false,"given":"Muhammad Tayyab","family":"Khan","sequence":"first","affiliation":[{"name":"Department of Theriogenology Faculty of Veterinary Science, University of Veterinary and Animal Sciences  Lahore Pakistan"},{"name":"Department of Clinical Sciences Faculty of Veterinary Science, Bahauddin Zakariya University  Multan Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ejaz","family":"Ahmad","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences Faculty of Veterinary Science, Bahauddin Zakariya University  Multan Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2432-5753","authenticated-orcid":false,"given":"Muhammad Irfan\u2010ur\u2010Rehman","family":"Khan","sequence":"additional","affiliation":[{"name":"Department of Theriogenology Faculty of Veterinary Science, University of Veterinary and Animal Sciences  Lahore Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,9,29]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2020.10.038"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2004.07.001"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4320(94)90069-8"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2004.09.005"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smallrumres.2024.107202"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.12750\/JARB.35.2.190"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2005.02.017"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.14579"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.14725"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2023.104593"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.21608\/javs.2020.118167"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smallrumres.2017.11.012"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.156.12.372"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4320(94)01328-J"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani12243590"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smallrumres.2021.106564"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/9781119110316.ch3"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.70129","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,8]],"date-time":"2025-12-08T13:26:48Z","timestamp":1765200408000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.70129"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9,29]]},"references-count":17,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2025,10]]}},"alternative-id":["10.1111\/rda.70129"],"URL":"https:\/\/doi.org\/10.1111\/rda.70129","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"type":"print","value":"0936-6768"},{"type":"electronic","value":"1439-0531"}],"subject":[],"published":{"date-parts":[[2025,9,29]]},"assertion":[{"value":"2025-05-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-22","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70129"}}