{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,8]],"date-time":"2025-12-08T13:29:33Z","timestamp":1765200573784,"version":"3.46.0"},"reference-count":56,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T00:00:00Z","timestamp":1761609600000},"content-version":"vor","delay-in-days":27,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Cystic ovarian disease is one of the leading causes of infertility in dairy cows. In this experimental field study, the comparative efficacy of the \u22123hCG\/Ovsynch protocol (pre\u2010treatment with hCG before Ovsynch) was investigated alongside the Ovsynch and the progestagen\u2010based Ovsynch protocol. A total of 45 dairy cows with follicular cyst (FC) were enrolled in the study. The cows were randomly divided into three equal groups: in the first group, the Ovsynch\u201048 protocol (Day 0 GnRH, Day 7 PGF\n                    <jats:sub>2\u03b1<\/jats:sub>\n                    , Day 9 GnRH and fixed\u2010time artificial insemination (FTAI) 16\u201320\u2009h later); in the second group, the CIDR\u2010synch protocol (Day 0 GnRH and the insertion of CIDR into the vagina for 7\u2009days, Day 7 PGF\n                    <jats:sub>2\u03b1<\/jats:sub>\n                    , Day 9 GnRH and FTAI 16\u201320\u2009h later); and in the third group, the \u22123hCG\/Ovsynch protocol (3000\u2009IU hCG pre\u2010treatment before initiation of the Ovsynch\u201048 protocol) were applied. Blood samples were collected on Day \u22123, 0, 3 and 7, and serum progesterone levels were measured. Transrectal sonography of the ovaries was performed on Day \u22123, 0, 3 and 7 for ovarian dynamics (follicular development, presence of FC, measurement of diameter and wall thickness of FC) throughout the protocols. Cows were fixed\u2010time artificially inseminated. On Day 13 (3\u2009days after FTAI) and Day 45 (pregnancy diagnosis), ovarian ultrasonography was also performed to evaluate the cystic persistence (PC). hCG pre\u2010treatment was found to be ineffective on follicular development in the early period of the \u22123hCG\/Ovsynch protocol, but it significantly increased the diameter of the dominant follicle on Day 3 when compared to the Ovsynch and CIDR\u2010synch protocols. The lifespan of the corpus luteum in the \u22123hCG\/Ovsynch group was longer (&gt;\u20094\u2009days) than in the other protocols (&lt;\u20094\u2009days). Following the Ovsynch, CIDR\u2010synch and \u22123hCG\/Ovsynch protocols, the PC rates were found to be 54.5% (6\/11), 45.4% (5\/11) and 27.2% (3\/11) on Day 13, and 27.2% (3\/11), 27.2% (3\/11) and 9.1% (1\/11) on Day 45, respectively. Conception rates were found in Ovsynch (6\/15), CIDR\u2010synch (8\/14) and \u22123hCG\/Ovsynch (8\/15) protocols as 40%, 57.1% and 53.3%, respectively. According to the findings, it was concluded that the \u22123hCG\/Ovsynch protocol may be more effective than the Ovsynch protocol in the treatment of cows with FCs, can be used as an alternative to the CIDR\u2010synch protocol and can be preferred especially in cows with uterine infection detected early in the postpartum period and in cases where the use of exogenous progesterone is contraindicated.\n                  <\/jats:p>","DOI":"10.1111\/rda.70138","type":"journal-article","created":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T13:55:41Z","timestamp":1761659741000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Pre\u2010Treatment With\n                    <scp>hCG<\/scp>\n                    Before Ovsynch (\u2212\n                    <scp>3hCG<\/scp>\n                    \/Ovsynch) May be an Alternative to the Progestagen\u2010Based Ovsynch Protocol in Cows With Ovarian Follicular Cyst"],"prefix":"10.1111","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1365-1729","authenticated-orcid":false,"given":"Halef","family":"Do\u011fan","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine Tekirdag Namik Kemal University  Suleymanpasa\/Tekirdag Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7780-2982","authenticated-orcid":false,"given":"Metin","family":"Bayraktar","sequence":"additional","affiliation":[{"name":"Department of Zootechny, Faculty of Veterinary Medicine F\u0131rat University  Elazig Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1782-583X","authenticated-orcid":false,"given":"Metehan","family":"Kutlu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine Necmettin Erbakan University  Eregli\/Konya Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5532-0525","authenticated-orcid":false,"given":"Kudret","family":"Yenilmez","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine Tekirdag Namik Kemal University  Suleymanpasa\/Tekirdag Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2642-4931","authenticated-orcid":false,"given":"Hasan","family":"Dogan","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine Tekirdag Namik Kemal University  Suleymanpasa\/Tekirdag Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,10,28]]},"reference":[{"issue":"11","key":"e_1_2_10_2_1","first-page":"931","article-title":"Ovarian and Endocrine Responses Associated With the Treatment of Cystic Ovarian Follicles in Dairy Cows With Gonadotropin Releasing Hormone and Prostaglandin F2\u03b1, With or Without Exogenous Progesterone","volume":"45","author":"Ambrose D. 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