{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T22:52:37Z","timestamp":1789771957016,"version":"4.0.1"},"reference-count":41,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T00:00:00Z","timestamp":1762905600000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Reproductive efficiency in boars partly depends on semen quality. However, it is challenging to predict sperm fertility once acceptable quality endpoints have been met. This study aims to establish a link between different semen quality parameters and farm fertility outcomes in semen doses selected for commercial use. We analysed 105 ejaculates from 15 adult Pietrain boars, extended into 45\u2009mL artificial insemination (AI) doses. A total of 605 sows were inseminated (40.1\u2009\u00b1\u20097.8 females\/boar) and fertility, farrowing rate and prolificacy data were recorded. Sperm evaluation included sperm plasma analysis, kinematics, morphology, viability, acrosome integrity, apoptotic\u2010like changes, mitochondrial activity and DNA damage (DNA fragmentation poly\u2010ADP ribose polymerase 1, PARP1, together with its product PAR and its cleaved form cPARP). The sperm membrane and acrosome were evaluated using the short hypo\u2010osmotic swelling test (sHOST) and the osmotic resistance test (ORT). Fertility and farrowing rates exceeded 94%, with an average of 20.18\u2009\u00b1\u20092.03 piglets born\/litter (average born alive: 87.75%\u2009\u00b1\u20095.61%). Negative correlations were found between damaged acrosomes and ultrasound fertility (\u03c1\u2009=\u2009\u22120.240,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.021), farrowing rate (\u03c1\u2009=\u2009\u22120.244;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.019), and total born (\n                    <jats:italic>r<\/jats:italic>\n                    \u2009=\u2009\u22120.304;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.003). Average born alive was positively correlated with plasma seminal concentrations of protein (\n                    <jats:italic>r<\/jats:italic>\n                    \u2009=\u20090.273;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.009), fructose (\n                    <jats:italic>r<\/jats:italic>\n                    \u2009=\u20090.243;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.024) and cathepsine B (\u03c1\u2009=\u20090.257;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.029) but negatively correlated with apoptosis and DNA damage nuclear markers cPARP (\n                    <jats:italic>r<\/jats:italic>\n                    \u2009=\u2009\u22120.295,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.005) and PAR (\n                    <jats:italic>r<\/jats:italic>\n                    \u2009=\u2009\u22120.209,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.049). However, regression models only showed significant results for predicting the total born from damaged acrosomes and those born alive from cPARP, although the coefficients of determination were very low. Since semen quality and fertility were good, most parameters did not affect fertility outcomes. In agreement with previous studies, acrosomal damage would be a reliable predictor of reproductive outcomes, whereas cPARP would show potential as a novel biomarker.\n                  <\/jats:p>","DOI":"10.1111\/rda.70145","type":"journal-article","created":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T12:10:31Z","timestamp":1762949431000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Acrosomal Status and\n                    <scp>PARP<\/scp>\n                    \u20101 Nuclear Markers Could Improve Discrimination of Potential Fertility in Good\u2010Quality Boar Semen Doses"],"prefix":"10.1111","volume":"60","author":[{"given":"Raquel","family":"Ausejo\u2010Marcos","sequence":"first","affiliation":[{"name":"Department of Biotechnology R&amp;D Magapor S.L  Ejea de los Caballeros Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Miguel\u2010Jim\u00e9nez","sequence":"additional","affiliation":[{"name":"Department of Research and Development CiencIAnova Magapor  Zaragoza Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. Teresa","family":"Tejedor","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Embryology and Genetics, CIBERCV, Faculty of Veterinary Medicine University of Zaragoza  Zaragoza Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bel\u00e9n","family":"G\u00f3mez\u2010Gim\u00e9nez","sequence":"additional","affiliation":[{"name":"Institute of Animal Health and Cattle Development (INDEGSAL) University of Le\u00f3n  Le\u00f3n Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Soriano\u2010\u00dabeda","sequence":"additional","affiliation":[{"name":"Institute of Animal Health and Cattle Development (INDEGSAL) University of Le\u00f3n  Le\u00f3n Spain"},{"name":"Department of Veterinary Medicine, Surgery, and Anatomy (Animal Medicine and Surgery) University of Le\u00f3n  Le\u00f3n Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felipe","family":"Martinez\u2010Pastor","sequence":"additional","affiliation":[{"name":"Institute of Animal Health and Cattle Development (INDEGSAL) University of Le\u00f3n  Le\u00f3n Spain"},{"name":"Department of Molecular Biology (Cell Biology) University of Le\u00f3n  Le\u00f3n Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noelia","family":"Mendoza","sequence":"additional","affiliation":[{"name":"Department of Biotechnology R&amp;D Magapor S.L  Ejea de los Caballeros Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0256-1958","authenticated-orcid":false,"given":"Alejandro","family":"Vicente\u2010Carrillo","sequence":"additional","affiliation":[{"name":"Department of Research and Development CiencIAnova Magapor  Zaragoza Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William Fernando","family":"Hurtado","sequence":"additional","affiliation":[{"name":"Department of Animal Pathology, Obstetrics and Reproduction, Faculty of Veterinary Medicine University of Zaragoza  Zaragoza Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Celia \u00c1vila","family":"Holgu\u00edn","sequence":"additional","affiliation":[{"name":"Department of Animal Pathology, Obstetrics and Reproduction, Faculty of Veterinary Medicine University of Zaragoza  Zaragoza Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernardino","family":"Moreno","sequence":"additional","affiliation":[{"name":"Department of Animal Pathology, Obstetrics and Reproduction, Faculty of Veterinary Medicine University of Zaragoza  Zaragoza Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Victoria","family":"Falceto","sequence":"additional","affiliation":[{"name":"Department of Animal Pathology, Obstetrics and Reproduction, Faculty of 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