{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T08:59:34Z","timestamp":1767603574138,"version":"3.48.0"},"reference-count":53,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,12,4]],"date-time":"2025-12-04T00:00:00Z","timestamp":1764806400000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100010723","name":"Afyon Kocatepe \u00dcniversitesi","doi-asserted-by":"publisher","award":["24.VF.04"],"award-info":[{"award-number":["24.VF.04"]}],"id":[{"id":"10.13039\/100010723","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The objective of the study was to investigate the vanillic acid's (VA) protective effects, a phenolic compound, on the ram semen after freeze\u2013thaw. Semen was obtained from Raml\u0131\u00e7 rams and was diluted with control (0\u2009\u03bcg\/mL VA) and VA\u2010supplemented Tris\u2010based extenders at concentrations of 1, 10 and 50\u2009\u03bcg\/mL. The diluted semen was equilibrated for 2\u2009h at +4\u00b0C, filled into 0.25\u2009mL straws and frozen in liquid nitrogen vapour. It was then stored in a liquid nitrogen container at \u2212196\u00b0C. For analysis, the samples were thawed at 37\u00b0C for 30\u2009s in a water bath. There was no difference detected among total and progressive motility as well as velocity parameters (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&gt;\u20090.05) except for rapid progressive motility (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). The findings support the idea that VA has an outstanding effect on reducing DNA damage (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001). While there was no positive development with regard to total oxidant status (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&gt;\u20090.05), VA enhanced the antioxidant defences of total antioxidant status (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). VA administered at doses of 10 and 50\u2009\u03bcg increased total antioxidant status (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.01). Lipid peroxidation was not directly affected by VA application (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&gt;\u20090.05); otherwise, 10 and 50\u2009\u03bcg VA treatments showed a positive effect on viability (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001). Based on findings, it was concluded that although VA was put in the semen extender, it did not have an ameliorative potency on sperm motility and velocity properties except for specific sub\u2010parameters such as rapid progressive motility. All applied doses reduced DNA damage, and 10 and 50\u2009\u03bcg doses supported cellular viability.\n                  <\/jats:p>","DOI":"10.1111\/rda.70152","type":"journal-article","created":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T07:24:44Z","timestamp":1764919484000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Can Vanillic Acid, a Phenolic Substance, Be a Useful Tool to Prevent Oxidative Stress Induced by Freezing Ram Sperm?"],"prefix":"10.1111","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7586-0895","authenticated-orcid":false,"given":"Bar\u0131\u015f","family":"Denk","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Faculty of Veterinary Medicine Afyon Kocatepe University  Afyonkarahisar T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Murat","family":"K\u0131r\u0131kkulak","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine Afyon Kocatepe University  Afyonkarahisar T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u015e\u00fckr\u00fc","family":"G\u00fcng\u00f6r","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine Burdur Mehmet Akif Ersoy University  Burdur T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4838-1597","authenticated-orcid":false,"given":"Mehmet Fuat","family":"G\u00fclhan","sequence":"additional","affiliation":[{"name":"Department of Medicinal Aromatic Plants, Technical Sciences Vocational School Aksaray University  Aksaray T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammed Enes","family":"\u0130nan\u00e7","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine Burdur Mehmet Akif Ersoy University  Burdur T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fatih","family":"Avdatek","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine Afyon Kocatepe University  Afyonkarahisar T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deniz","family":"Yeni","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine Afyon Kocatepe University  Afyonkarahisar T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2827-1286","authenticated-orcid":false,"given":"Umut","family":"Ta\u015fdemir","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine Ankara University  Ankara T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,12,4]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.2007.00559.x"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2005.12.026"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.13523"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2024.1394273"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.109.076471"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.4061\/2011\/686137"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25031892"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2018.0118"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.2174\/138161210790883615"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jnatprod.5b00246"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani14020251"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.12506"},{"key":"e_1_2_12_14_1","first-page":"421","volume-title":"Methods in Enzymology","author":"Draper H. 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