{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T08:59:34Z","timestamp":1767603574540,"version":"3.48.0"},"reference-count":27,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T00:00:00Z","timestamp":1766966400000},"content-version":"vor","delay-in-days":28,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The present study investigated the relationship between seminal parameters and bull conception rate (BCR) in 72 Murrah bulls using Computer\u2010Assisted Sperm Analysis (CASA). The BCR was calculated by obtaining data of artificial insemination spanned over two decades from buffalo farms of two organised herds of India. The association of seminal parameters and BCR was studied using multiple regression and principal component analysis. The average BCR was 38.95%\u2009\u00b1\u20091.51%, ranging from 22.50% to 80.51%. Total motility (TM), progressive motility (PM), average path velocity (VAP), straight line velocity (VSL), curvilinear velocity (VCL), and amplitude of lateral head displacement (ALH) showed significant positive correlations with BCR, whereas straightness (STR) and linearity (LIN) were negatively correlated. Multiple regression (R\n                    <jats:sup>2<\/jats:sup>\n                    \u2009=\u20090.29) identified TM as the most reliable predictor of BCR. Principal component analysis (PCA) extracted three components, PC1 (Sperm velocity and head movement, 54.36% variance), PC2 (Trajectory and beat frequency, 19.56%), and PC3 (Progressive motility and path accuracy, 12.98%), explaining 86.90% of the total variance. Regression using PC scores (R\n                    <jats:sup>2<\/jats:sup>\n                    \u2009=\u20090.24) indicated positive effects of PC1 and PC3, and a negative effect of PC2 on fertility. Overall, sperm velocity and progressive motility were primary fertility determinants, while excessive linearity hindered conception success. Therefore, association of seminal parameters with BCR can be explored for enhancing breeding efficiency of bulls. Future breeding programmes should prioritise sperm velocity and progressive motility traits while avoiding excessive linearity to improve bull fertility and conception success.\n                  <\/jats:p>","DOI":"10.1111\/rda.70153","type":"journal-article","created":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T12:49:12Z","timestamp":1767012552000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Association of\n                    <scp>CASA<\/scp>\n                    \u2010Derived Semen Parameters With Conception Rate in Murrah Bulls"],"prefix":"10.1111","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7923-0316","authenticated-orcid":false,"given":"Komal","family":"Jaglan","sequence":"first","affiliation":[{"name":"College of Veterinary Sciences Lala Lajpat Rai University of Veterinary and Animal Sciences  Hisar India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. S.","family":"Dhaka","sequence":"additional","affiliation":[{"name":"College of Veterinary Sciences Lala Lajpat Rai University of Veterinary and Animal Sciences  Hisar India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sanjay","family":"Kumar","sequence":"additional","affiliation":[{"name":"ICAR\u2010Central Institute for Research on Buffaloes  Hisar India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. S.","family":"Patil","sequence":"additional","affiliation":[{"name":"College of Veterinary Sciences Rani Lakshmibai Central Agricultural University  Jhansi Uttar Pradesh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8861-161X","authenticated-orcid":false,"given":"Y. C.","family":"Bangar","sequence":"additional","affiliation":[{"name":"College of Veterinary Sciences Lala Lajpat Rai University of Veterinary and Animal Sciences  Hisar India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3930-2201","authenticated-orcid":false,"given":"Pradeep","family":"Kumar","sequence":"additional","affiliation":[{"name":"ICAR\u2010Central Institute for Research on Buffaloes  Hisar India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renu","family":"Bala","sequence":"additional","affiliation":[{"name":"ICAR\u2010Central Institute for Research on Buffaloes  Hisar India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,12,29]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.12639"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11250-024-03975-3"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani14010132"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2024-25163"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/biology10070595"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1751731117000684"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.3906\/vet-1801-83"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.56093\/ijans.v86i6.59160"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.56093\/ijans.v84i4.39838"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.48165\/ijar.2022.43.1.3"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/09064702.2024.2382130"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2015.05.010"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1556\/004.2015.035"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2013.01.010"},{"issue":"10","key":"e_1_2_12_16_1","first-page":"1188","article-title":"Comparative Evaluation of Motility and Kinematics of Fresh Versus Frozen\u2010Thawed Spermatozoa of Cattle and Buffalo Bull by CASA","volume":"54","author":"Pathak P. K.","year":"2020","journal-title":"Indian Journal of Animal Research"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1088\/1755\u20101315\/788\/1\/012137"},{"issue":"1","key":"e_1_2_12_18_1","first-page":"21","article-title":"Relationship Between Semen Quality Parameters and Field Fertility of Bulls","volume":"27","author":"Rabidas S. K.","year":"2012","journal-title":"Journal of Embryo Transfer"},{"key":"e_1_2_12_19_1","unstructured":"Rafiq M. M.2022.\u201cEffect of Age on Lipid Composition and Semen Quality in Murrah Buffalo Bulls.\u201dPh.D. diss. National Dairy Research Institute (NDRI) Karnal India."},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0237766"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.56093\/ijans.v91i6.115435"},{"issue":"4","key":"e_1_2_12_22_1","first-page":"660","article-title":"Computer\u2010Assisted Sperm Analysis: Relationship Between the Movement Characteristics of Buffalo Spermatozoa and Sire Fertility","volume":"51","author":"Singh R. K.","year":"2017","journal-title":"Indian Journal of Animal Research"},{"issue":"1","key":"e_1_2_12_23_1","first-page":"87","article-title":"Seasonal Variation of Sperm Kinematics in Murrah Bulls Under the Tropical Climatic Condition","volume":"40","author":"Sinha R.","year":"2021","journal-title":"Buffalo Bulletin"},{"issue":"1","key":"e_1_2_12_24_1","first-page":"53","article-title":"Assessment of Buffalo Bull Semen Quality Based on Sperm Motility Parameters, Motion Characteristics and In\u00a0Vitro Fertilization Rate","volume":"33","author":"Sohail A.","year":"2013","journal-title":"Pakistan Veterinary Journal"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.livsci.2018.12.008"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/9781118833971.ch74"},{"issue":"2","key":"e_1_2_12_27_1","article-title":"Relationship Between In\u00a0Vitro Semen Parameters and Bull Fertility","volume":"10","author":"Zahan M.","year":"2018","journal-title":"Animal Biology and Animal Husbandry"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2019.11.021"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.70153","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T08:58:09Z","timestamp":1767603489000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.70153"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12]]},"references-count":27,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["10.1111\/rda.70153"],"URL":"https:\/\/doi.org\/10.1111\/rda.70153","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"type":"print","value":"0936-6768"},{"type":"electronic","value":"1439-0531"}],"subject":[],"published":{"date-parts":[[2025,12]]},"assertion":[{"value":"2025-08-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70153"}}