{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T06:59:22Z","timestamp":1773817162513,"version":"3.50.1"},"reference-count":43,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T00:00:00Z","timestamp":1768348800000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32160788"],"award-info":[{"award-number":["32160788"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>In vitro maturation (IVM) of oocytes is crucial in livestock breeding. Oocytes obtained by IVM are more susceptible to oxidative stress than in\u00a0vivo, leading to low maturation rates. Betaine from red beetroot acts as an antioxidant and methyl donor, regulating epigenetic modifications in cell physiology. This study investigates Betaine's effects on porcine oocyte IVM, embryo development and underlying molecular mechanisms. Results demonstrate that 16\u2009mmol\/L Betaine significantly enhances the first polar body extrusion, cleavage and blastocyst rates compared to the control and other concentrations. Betaine elevates normal cortical granule distribution, normal spindle assembly, normal chromosome arrangement and overall m6A levels during IVM. It increases the antioxidant gene expression and mitochondrial function and decreases reactive oxygen species levels. However, Betaine's beneficial effects were diminished by AMPK inhibitor compound C. In conclusion, Betaine enhances porcine oocyte IVM and early embryo development by enhancing the antioxidant capacity and mitochondrial function pathway.<\/jats:p>","DOI":"10.1111\/rda.70157","type":"journal-article","created":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T15:28:30Z","timestamp":1768404510000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effects of Betaine on\n                    <scp>m6A<\/scp>\n                    Methylation,\n                    <scp>AMPK<\/scp>\n                    Signalling and Cytoplasmic Maturation in Porcine Oocytes"],"prefix":"10.1111","volume":"61","author":[{"given":"Xi","family":"Yan","sequence":"first","affiliation":[{"name":"Guangxi Key Laboratory of Livestock and Poultry Breeding and Disease Prevention and Control, Animal Reproduction Institute Guangxi University  Nanning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3014-2504","authenticated-orcid":false,"given":"Yanxin","family":"Wang","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Livestock and Poultry Breeding and Disease Prevention and Control, Animal Reproduction Institute Guangxi University  Nanning China"},{"name":"Research Center for Environment and Female Reproductive Health, the Eighth Affiliated Hospital Sun Yat\u2010Sen University  Shenzhen China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-8644-4063","authenticated-orcid":false,"given":"Yun","family":"Wang","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Livestock and Poultry Breeding and Disease Prevention and Control, Animal Reproduction Institute Guangxi University  Nanning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruru","family":"Jia","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Livestock and Poultry Breeding and Disease Prevention and Control, Animal Reproduction Institute Guangxi University  Nanning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sijia","family":"Li","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Livestock and Poultry Breeding and Disease Prevention and Control, Animal Reproduction Institute Guangxi University  Nanning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mosinan","family":"Chen","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Livestock and Poultry Breeding and Disease Prevention and Control, Animal Reproduction Institute Guangxi University  Nanning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deshun","family":"Shi","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Livestock and Poultry Breeding and Disease Prevention and Control, Animal Reproduction Institute Guangxi University  Nanning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fenghua","family":"Lu","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Livestock and Poultry Breeding and Disease Prevention and Control, Animal Reproduction Institute Guangxi University  Nanning China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,1,14]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477\u20107827\u20103\u201028"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1080350105"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1080340406"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox12122117"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/s1472\u20106483(10)60038\u20107"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.113.116939"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules28124824"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.21229"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.2903\/j.efsa.2018.5335"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpn.13633"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/dmcn.15332"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.20127"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.22725"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12192\u2010019\u201001022\u2010x"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2023.102928"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00400.2021"},{"key":"e_1_2_13_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2022.02.004"},{"key":"e_1_2_13_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10815\u2010020\u201002014\u2010y"},{"key":"e_1_2_13_20_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M117.803080"},{"key":"e_1_2_13_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11250\u2010017\u20101355\u2010z"},{"key":"e_1_2_13_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2022.869950"},{"key":"e_1_2_13_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21031135"},{"key":"e_1_2_13_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2022.11.048"},{"key":"e_1_2_13_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10545\u2010010\u20109088\u20104"},{"key":"e_1_2_13_26_1","doi-asserted-by":"publisher","DOI":"10.1039\/c9fo01853k"},{"key":"e_1_2_13_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.30468"},{"key":"e_1_2_13_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2018.08.009"},{"key":"e_1_2_13_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox12061222"},{"key":"e_1_2_13_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani12192508"},{"key":"e_1_2_13_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep41606"},{"key":"e_1_2_13_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13048\u2010022\u201001032\u2010x"},{"key":"e_1_2_13_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms26083624"},{"key":"e_1_2_13_34_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/6545728"},{"key":"e_1_2_13_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2023.10.392"},{"key":"e_1_2_13_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10565\u2010025\u201010093\u20109"},{"key":"e_1_2_13_37_1","doi-asserted-by":"publisher","DOI":"10.3390\/jpm13081264"},{"key":"e_1_2_13_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.psj.2023.102710"},{"key":"e_1_2_13_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010018\u201020175\u2010w"},{"key":"e_1_2_13_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2023.135445"},{"key":"e_1_2_13_41_1","doi-asserted-by":"publisher","DOI":"10.1039\/c8fo02004c"},{"key":"e_1_2_13_42_1","doi-asserted-by":"publisher","DOI":"10.4149\/neo_2022_220714N714"},{"key":"e_1_2_13_43_1","doi-asserted-by":"publisher","DOI":"10.1093\/nsr\/nwae039"},{"key":"e_1_2_13_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419\u2010022\u201004503\u20107"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.70157","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/rda.70157","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.70157","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T05:58:46Z","timestamp":1773813526000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.70157"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":43,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1111\/rda.70157"],"URL":"https:\/\/doi.org\/10.1111\/rda.70157","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"2025-08-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70157"}}