{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T15:12:54Z","timestamp":1789917174469,"version":"4.0.1"},"reference-count":43,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T00:00:00Z","timestamp":1769558400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T00:00:00Z","timestamp":1769558400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The aims of this study were to assess the effects of resveratrol\u2010loaded nanoparticles (RLNP) on follicular survival, stromal integrity and activity of endogenous free\u2010radical scavengers in bovine ovarian tissues cultured in\u00a0vitro. Ovarian cortical slices were incubated in \u03b1\u2010MEM\n                    <jats:sup>+<\/jats:sup>\n                    alone or supplemented with 0.2, 2.0 or 20.0\u2009\u03bcM RLNP, blank nanoparticles (BNP) or free resveratrol (20.0\u2009\u03bcM) for 6\u2009days at 38.5\u00b0C and 5% CO\n                    <jats:sub>2<\/jats:sub>\n                    . Follicular integrity, number of stromal cells, density of collagen fibres, levels of thiol and activity of free\u2010radical scavengers (glutathione peroxidase [GPX], superoxide dismutase [SOD] and catalase [CAT]) were evaluated in tissues cultured in the different treatments. The data showed that ovarian cortex cultured with 20.0\u2009\u03bcM free resveratrol or RLNP, in all tested concentrations, had a reduced rate of morphologically intact follicles in relation to uncultured controls (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). The RLNP (0.2, 2.0 or 20.0\u2009\u03bcM) and BNP increased the proportion of growing follicles and stromal cell numbers (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). Collagen fibre levels decreased in tissues cultured with 0.2 or 2.0\u2009\u03bcM RLNP compared to uncultured controls, but remained greater than those seen in ovarian cortex cultured in other treatments (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). Free resveratrol increased CAT and GPX activity, while RLNP reduced activity of SOD and GPX (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). In conclusion, RLNP improved follicle survival and growth, preserved stromal tissue and modulated the activity of free\u2010radical scavengers in bovine ovarian slices cultured in\u00a0vitro.\n                  <\/jats:p>","DOI":"10.1111\/rda.70158","type":"journal-article","created":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T09:37:10Z","timestamp":1769593030000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Effects of Resveratrol\u2010Loaded Nanoparticles on Follicular Survival, Stromal Integrity and Activity of Endogenous Free\u2010Radical Scavengers in Bovine Ovarian Cortical Slices Cultured In\u00a0Vitro"],"prefix":"10.1111","volume":"61","author":[{"given":"Mara B. A.","family":"Catunda","sequence":"first","affiliation":[{"name":"Laboratory of Biotechnology and Physiology of Reproduction Federal University of Cear\u00e1  Sobral Cear\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco das C.","family":"Costa","sequence":"additional","affiliation":[{"name":"Laboratory of Biotechnology and Physiology of Reproduction Federal University of Cear\u00e1  Sobral Cear\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vit\u00f3ria S.","family":"Bezerra","sequence":"additional","affiliation":[{"name":"Laboratory of Biotechnology and Physiology of Reproduction Federal University of Cear\u00e1  Sobral Cear\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco F.","family":"Caetano Filho","sequence":"additional","affiliation":[{"name":"Laboratory of Biotechnology and Physiology of Reproduction Federal University of Cear\u00e1  Sobral Cear\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Regislane P.","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Laboratory of Biotechnology and Physiology of Reproduction Federal University of Cear\u00e1  Sobral Cear\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreza de A.","family":"Silva","sequence":"additional","affiliation":[{"name":"Laboratory of Biotechnology and Physiology of Reproduction Federal University of Cear\u00e1  Sobral Cear\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Solano D.","family":"Martins","sequence":"additional","affiliation":[{"name":"Reproductive Physiology Research Laboratory Federal University of Delta do Parna\u00edba \u2010 UFDPar  Parna\u00edba Piau\u00ed Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valdevane R.","family":"Ara\u00fajo","sequence":"additional","affiliation":[{"name":"Reproductive Physiology Research Laboratory Federal University of Delta do Parna\u00edba \u2010 UFDPar  Parna\u00edba Piau\u00ed Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alice V. F.","family":"Reis","sequence":"additional","affiliation":[{"name":"Center for Pharmaceutical Development and Testing Federal University of Cear\u00e1  Fortaleza Cear\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josimar O.","family":"Eloy","sequence":"additional","affiliation":[{"name":"Center for Pharmaceutical Development and Testing Federal University of Cear\u00e1  Fortaleza Cear\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5970-6177","authenticated-orcid":false,"given":"Jos\u00e9 R. V.","family":"Silva","sequence":"additional","affiliation":[{"name":"Laboratory of Biotechnology and Physiology of Reproduction Federal University of Cear\u00e1  Sobral Cear\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,28]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph17010126"},{"issue":"2","key":"e_1_2_11_3_1","first-page":"6","article-title":"Resveratrol Atenua a Atrofia e a Perda de \u00c1cinos da Gl\u00e2ndula Salivar Par\u00f3tida de Ratos com Diabetes Cr\u00f4nico","volume":"30","author":"Alves A. M. P.","year":"2017","journal-title":"Revista UNING\u00c1"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/acel.13259"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/cnr2.1353"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.13274"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.13565"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2025.01.020"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(59)90090-6"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.5935\/1678\u20104596.20170042"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.2174\/1874609815666220511143843"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers15102802"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2019.05.008"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dead008"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2017.11.009"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/der468"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2012.01.024"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12951\u2010022\u201001890\u20104"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1080\/10717544.2023.2174206"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1080\/09513590.2023.2181652"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1039\/c8fo01172a"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.2147\/DDDT.S248950"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics16060761"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477\u20107827\u201010\u201014"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2020.118555"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2012.08.004"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s43032\u2010021\u201000840\u20108"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2020.111793"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2012.509"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2020.08.016"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/s11658\u2010019\u20100174\u20103"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u20101\u20104939\u20107113\u20108_26"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2018.04.004"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10815\u2010018\u20101180\u2010y"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2023.06.015"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2024.1347286"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.5935\/1518\u20100557.20210076"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1080\/00021369.1978.10863059"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2021.03.004"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2007.01.011"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12958\u2010021\u201000735\u2010y"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2013.10.041"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.4115"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.70158","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/rda.70158","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.70158","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T03:25:45Z","timestamp":1773026745000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.70158"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,28]]},"references-count":43,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["10.1111\/rda.70158"],"URL":"https:\/\/doi.org\/10.1111\/rda.70158","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,28]]},"assertion":[{"value":"2025-05-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-28","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70158"}}