{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T07:18:38Z","timestamp":1780471118995,"version":"3.54.1"},"reference-count":22,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T00:00:00Z","timestamp":1770854400000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100014750","name":"Universidad San Pablo - CEU","doi-asserted-by":"publisher","award":["GIR24\u201012"],"award-info":[{"award-number":["GIR24\u201012"]}],"id":[{"id":"10.13039\/501100014750","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Transvaginal ultrasound\u2010guided follicular aspiration or ovum pick\u2010up (OPU) has become the standard technique for oocyte collection in mares for intracytoplasmic sperm injection (ICSI). Although repeated follicular flushes and wall scraping are commonly used to improve oocyte recovery rate (ORR), the relative contribution of each remains unclear. This study aimed to compare the effects of multiple flushes versus controlled scraping time on ORR in mares. A controlled trial was conducted in two phases: (1) an ex\u00a0vivo phase using slaughterhouse ovaries (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200932), and (2) an in\u00a0vivo phase in clinically healthy mares (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20099). Follicles were assigned to two groups: multiple flushes (MF, 10 flushes with intermittent scraping, lasting 18\u2009s in total) or single flush (SF, 18\u2009s of continuous scraping followed by 1 flush). A total of 489 follicles were aspirated ex\u00a0vivo and 143 in\u00a0vivo. Results showed no significant difference in ORR between MF and SF groups in either phase (ex vivo: 63.8% vs. 59.5%; in\u00a0vivo: 48.3% vs. 44.3%;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&gt;\u20090.05). However, MF used significantly more flushing medium per follicle in the ex\u00a0vivo model (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). No significant differences were found in aspiration time or incidence of clots and blockages in the aspiration lines. A significant correlation was observed between clot number and aspiration system blockage (\n                    <jats:italic>r<\/jats:italic>\n                    \u2009=\u20090.497,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.036). Continuous scraping without repeated flushing reduces medium usage but may increase operator fatigue and the risk of clot formation and system obstructions. Therefore, optimising scraping duration could improve the efficiency of oocyte recovery procedures while simplifying the technique and reducing costs. Further research is required to refine scraping protocols, reduce operator fatigue, and prevent complications related to clot formation.\n                  <\/jats:p>","DOI":"10.1111\/rda.70183","type":"journal-article","created":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T09:21:21Z","timestamp":1770888081000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Comparative Evaluation of Follicular Flushing Frequency and Scraping Time During Ovum Pick\u2010Up in Mares: Effects on Oocyte Recovery Rate and Technical Considerations"],"prefix":"10.1111","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-1569-5843","authenticated-orcid":false,"given":"Adri\u00e1n","family":"M\u00e1rquez\u2010Moya","sequence":"first","affiliation":[{"name":"Equine Fertility Group, Facultad de Veterinaria Universidad Cardenal Herrera\u2010CEU, CEU Universities  Alfara del Patriarca Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-4564-5653","authenticated-orcid":false,"given":"Nerea","family":"Carreras\u2010Vico","sequence":"additional","affiliation":[{"name":"Equine Fertility Group, Facultad de Veterinaria Universidad Cardenal Herrera\u2010CEU, CEU Universities  Alfara del Patriarca Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Sala\u2010Ayala","sequence":"additional","affiliation":[{"name":"Equine Fertility Group, Facultad de Veterinaria Universidad Cardenal Herrera\u2010CEU, CEU Universities  Alfara del Patriarca Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebeca","family":"Mart\u00ednez\u2010Bov\u00ed","sequence":"additional","affiliation":[{"name":"Equine Fertility Group, Facultad de Veterinaria Universidad Cardenal Herrera\u2010CEU, CEU Universities  Alfara del Patriarca Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6939-017X","authenticated-orcid":false,"given":"Juan","family":"Cuervo\u2010Arango","sequence":"additional","affiliation":[{"name":"Equine Fertility Group, Facultad de Veterinaria Universidad Cardenal Herrera\u2010CEU, CEU Universities  Alfara del Patriarca Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,12]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042\u20103306.1997.tb05099.x"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.14625"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004290050205"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042\u20103306.1992.tb02780.x"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2022.04.019"},{"key":"e_1_2_12_7_1","first-page":"554","volume-title":"Proceedings of the 53rd Annual Convention of the American Association of Equine Practitioners","author":"Colleoni S.","year":"2007"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2019.04.010"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1071\/rd19201"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.70043"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani15060832"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/biolre\/ioac172"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2024.04.007"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2019.03.007"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/biolreprod\/52.monograph_series1.243"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jevs.2025.105363"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jevs.2020.103097"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2024.12.032"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jevs.2020.103324"},{"key":"e_1_2_12_20_1","volume-title":"The Principles of Humane Experimental Technique","author":"Russell W. 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